PMID- 24693261
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140402
LR  - 20211021
IS  - 1544-2896 (Print)
IS  - 1544-2896 (Electronic)
IS  - 1544-2896 (Linking)
VI  - 12
IP  - 2
DP  - 2014 Spring
TI  - Application of a spike sorting procedure to analyze recordings in the crayfish 
      ventral superficial flexor preparation: A high resolution approach to the study 
      of neuromodulators on axons and synapses.
PG  - A140-9
AB  - The crayfish ventral superficial flexor (VSF) is innervated by the ventral branch 
      of the third nerve. This nerve and muscle system is tonic, and is responsible for 
      posture control of the animal. The easy accessibility of the third nerve and its 
      muscle fibers, along with its display of tonic activity in vitro, make this 
      preparation ideal for observing axonal action potentials and synaptic responses. 
      As a result, this preparation has often been adopted for undergraduate 
      electrophysiology laboratories. This report describes application of a spike 
      sorting procedure to simultaneously recorded traces from the third nerve and 
      associated muscle fiber. This procedure allows for isolation of action potentials 
      arising from each of the six axons in the third nerve. Separation of action 
      potentials and their corresponding synaptic responses from large data sets, with 
      sample sizes in the thousands, enables us to perform averaging and to analyze 
      waveforms of action potentials and synaptic responses with a high resolution. 
      With this high resolution approach, we document variations in the shape of action 
      potentials in the third nerve and in synaptic potential in muscle fibers. The 
      approach described here can be used for detailed study of the effects mediated by 
      neuromodulators and drugs. An example of such an application is illustrated using 
      50 muM GABA. Several possible student projects using this approach are outlined 
      and discussed.
FAU - Inam, Zaina Sohail
AU  - Inam ZS
AD  - Department of Biology, Boston University, Boston, MA, 02215.
FAU - Nelamangala, Shruti Kamala
AU  - Nelamangala SK
AD  - Department of Biology, Boston University, Boston, MA, 02215.
FAU - Lin, Jen-Wei
AU  - Lin JW
AD  - Department of Biology, Boston University, Boston, MA, 02215.
LA  - eng
PT  - Journal Article
DEP - 20140315
PL  - United States
TA  - J Undergrad Neurosci Educ
JT  - Journal of undergraduate neuroscience education : JUNE : a publication of FUN, 
      Faculty for Undergraduate Neuroscience
JID - 101229224
PMC - PMC3970996
OTO - NOTNLM
OT  - GABA
OT  - action potential
OT  - crayfish
OT  - neuromuscular junction
OT  - spike sorting
OT  - synaptic transmission
OT  - ventral superficial flexor
EDAT- 2014/04/03 06:00
MHDA- 2014/04/03 06:01
PMCR- 2014/03/15
CRDT- 2014/04/03 06:00
PHST- 2013/12/17 00:00 [received]
PHST- 2014/02/07 00:00 [revised]
PHST- 2014/02/14 00:00 [accepted]
PHST- 2014/04/03 06:00 [entrez]
PHST- 2014/04/03 06:00 [pubmed]
PHST- 2014/04/03 06:01 [medline]
PHST- 2014/03/15 00:00 [pmc-release]
AID - june-12-140 [pii]
PST - epublish
SO  - J Undergrad Neurosci Educ. 2014 Mar 15;12(2):A140-9. eCollection 2014 Spring.