PMID- 24693965
OWN - NLM
STAT- MEDLINE
DCOM- 20140630
LR  - 20211021
IS  - 1945-7170 (Electronic)
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 155
IP  - 6
DP  - 2014 Jun
TI  - Epidermal growth factor receptor (EGFR) signaling is a key mediator of 
      hormone-induced leukocyte infiltration in the pubertal female mammary gland.
PG  - 2301-13
LID - 10.1210/en.2013-1933 [doi]
AB  - It is well documented that macrophages and eosinophils play important roles in 
      normal murine pubertal mammary gland development. Although it is accepted that 
      estrogen (E) and progesterone (P) are key players in mammary gland development, 
      the roles these hormones might play in regulating the actions of leukocytes in 
      that process is an understudied area. We show here that P and E, respectively, 
      induce unique, but overlapping, sets of proinflammatory and angiogenic cytokines 
      and chemokines, in the pubertal female BALB/c mammary gland, as well as induce 
      infiltration of macrophages and eosinophils to the mammary periepithelium. This 
      extends earlier studies showing P induction of proinflammatory products in 
      pubertal and adult mammary epithelial organoids and P-induced in vivo 
      infiltration of leukocytes to the adult mammary periepithelium. Importantly, 
      epidermal growth factor receptor-signaling, which is likely mediated by 
      amphiregulin (Areg), a downstream mediator of E and P, is both necessary and 
      sufficient for both E- and P-induced recruitment of macrophages and eosinophils 
      to the pubertal mammary periepithelium. We further show that receptor activator 
      of nuclear factor kappaB ligand (RANKL), although not sufficient of itself to cause 
      macrophage and eosinophil recruitment, contributes to an optimal response to P. 
      The potency of Areg is highlighted by the fact that it is sufficient to induce 
      macrophage and eosinophil recruitment at levels equivalent to that induced by 
      either E or P. Our finding of a dominant role for Areg in hormonally induced 
      leukocyte recruitment to the pubertal mammary gland parallels its dominance in 
      regulating ductal outgrowth and its role in P-induced proliferation in the 
      pubertal gland.
FAU - Aupperlee, Mark D
AU  - Aupperlee MD
AD  - Breast Cancer and the Environment Research Program, Departments of Physiology 
      (M.D.A., Y.Z., Y.S.T., J.R.L., J.B., S.Z.H.) and Microbiology and Molecular 
      Genetics (R.C.S.), Michigan State University, East Lansing, Michigan 48824.
FAU - Zhao, Yong
AU  - Zhao Y
FAU - Tan, Ying Siow
AU  - Tan YS
FAU - Leipprandt, Jeffrey R
AU  - Leipprandt JR
FAU - Bennett, Jessica
AU  - Bennett J
FAU - Haslam, Sandra Z
AU  - Haslam SZ
FAU - Schwartz, Richard C
AU  - Schwartz RC
LA  - eng
GR  - U01 ES012800/ES/NIEHS NIH HHS/United States
GR  - U01 ES019434/ES/NIEHS NIH HHS/United States
GR  - 1U01ES12800/ES/NIEHS NIH HHS/United States
GR  - 1U01ESO19434/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140402
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Estrogens)
RN  - 0 (Isoenzymes)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 3.1.3.2 (Acid Phosphatase)
RN  - EC 3.1.3.2 (Tartrate-Resistant Acid Phosphatase)
SB  - IM
MH  - Acid Phosphatase/pharmacology
MH  - Animals
MH  - ErbB Receptors/genetics/*metabolism
MH  - Estrogens/*pharmacology
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Isoenzymes/pharmacology
MH  - Leukocytes/*cytology
MH  - Mammary Glands, Animal/*drug effects/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Progesterone/*pharmacology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction/drug effects
MH  - Tartrate-Resistant Acid Phosphatase
PMC - PMC4020926
EDAT- 2014/04/04 06:00
MHDA- 2014/07/01 06:00
PMCR- 2015/06/01
CRDT- 2014/04/04 06:00
PHST- 2014/04/04 06:00 [entrez]
PHST- 2014/04/04 06:00 [pubmed]
PHST- 2014/07/01 06:00 [medline]
PHST- 2015/06/01 00:00 [pmc-release]
AID - EN-13-1933 [pii]
AID - 10.1210/en.2013-1933 [doi]
PST - ppublish
SO  - Endocrinology. 2014 Jun;155(6):2301-13. doi: 10.1210/en.2013-1933. Epub 2014 Apr 
      2.