PMID- 24694566
OWN - NLM
STAT- MEDLINE
DCOM- 20150130
LR  - 20140415
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 95
DP  - 2014 Jul
TI  - Chemical comparison of Tripterygium wilfordii and Tripterygium hypoglaucum based 
      on quantitative analysis and chemometrics methods.
PG  - 220-8
LID - S0731-7085(14)00134-4 [pii]
LID - 10.1016/j.jpba.2014.03.009 [doi]
AB  - Tripterygium wilfordii (T. wilfordii) and Tripterygium hypoglaucum (T. 
      hypoglaucum), two commonly used Chinese herbal medicines derived from 
      Tripterygium genus, have been widely used for the treatment of rheumatoid 
      arthritis and other related inflammatory diseases in clinical therapy. In the 
      present study, a rapid resolution liquid chromatography/electrospray ionization 
      tandem mass spectrometry (RRLC-ESI-MS(n)) method has been developed and validated 
      for simultaneous determination of 19 bioactive compounds including four 
      catechins, three sesquiterpene alkaloids, four diterpenoids, and eight 
      triterpenoids in these two similar herbs. The method validation results indicated 
      that the developed method had desirable specificity, linearity, precision and 
      accuracy. Quantitative analysis results showed that there were significant 
      differences in the content of different types of compounds in T. wilfordii and T. 
      hypoglaucum. Moreover, chemometrics methods such as one-way ANOVA, principal 
      component analysis (PCA) and hierarchical clustering analysis (HCA) were 
      performed to compare and discriminate the two Tripterygium herbs based on the 
      quantitative data of analytes, and it was proven straightforward and reliable to 
      differentiate T. wilfordii and T. hypoglaucum samples from different origins. In 
      conclusion, simultaneous quantification of multiple-active component by 
      RRLC-ESI-MS(n) coupled with chemometrics analysis could be a well-acceptable 
      strategy to compare and evaluate the quality of T. wilfordii and T. hypoglaucum.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Guo, Long
AU  - Guo L
AD  - State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 
      24 Tongjia Lane, Nanjing 210009, China.
FAU - Duan, Li
AU  - Duan L
AD  - State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 
      24 Tongjia Lane, Nanjing 210009, China.
FAU - Liu, Ke
AU  - Liu K
AD  - State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 
      24 Tongjia Lane, Nanjing 210009, China.
FAU - Liu, E-Hu
AU  - Liu EH
AD  - State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 
      24 Tongjia Lane, Nanjing 210009, China. Electronic address: liuehu2011@163.com.
FAU - Li, Ping
AU  - Li P
AD  - State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 
      24 Tongjia Lane, Nanjing 210009, China. Electronic address: liping2004@126.com.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140315
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
SB  - IM
MH  - Calibration
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Principal Component Analysis
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Tripterygium/*chemistry
OTO - NOTNLM
OT  - Chemometrics
OT  - Quantitative analysis
OT  - RRLC-ESI-MS(n)
OT  - Tripterygium hypoglaucum
OT  - Tripterygium wilfordii
EDAT- 2014/04/04 06:00
MHDA- 2015/01/31 06:00
CRDT- 2014/04/04 06:00
PHST- 2013/12/18 00:00 [received]
PHST- 2014/03/03 00:00 [revised]
PHST- 2014/03/07 00:00 [accepted]
PHST- 2014/04/04 06:00 [entrez]
PHST- 2014/04/04 06:00 [pubmed]
PHST- 2015/01/31 06:00 [medline]
AID - S0731-7085(14)00134-4 [pii]
AID - 10.1016/j.jpba.2014.03.009 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2014 Jul;95:220-8. doi: 10.1016/j.jpba.2014.03.009. Epub 
      2014 Mar 15.