PMID- 24695322
OWN - NLM
STAT- MEDLINE
DCOM- 20150120
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 4
DP  - 2014
TI  - CD8alpha dendritic cells drive establishment of HSV-1 latency.
PG  - e93444
LID - 10.1371/journal.pone.0093444 [doi]
LID - e93444
AB  - It is generally accepted that CD8 T cells play the key role to maintain HSV-1 
      latency in trigeminal ganglia of ocularly infected mice. Yet, comparably little 
      is known about the role of innate immunity in establishment of viral latency. In 
      the current study, we investigated whether CD8alpha DCs impact HSV-1 latency by 
      examining latency in the trigeminal ganglia (TG) of wild-type (WT) C57BL/6 versus 
      CD8alpha-/- (lack functional CD8 T cells and CD8alpha+ DCs), CD8beta-/- (have functional 
      CD8alpha+ T cells and CD8alpha+ DCs), and beta2m-/- (lack functional CD8 T cells but have 
      CD8alpha+ DCs) mice as well as BXH2 (have functional CD8 T cells but lack CD8alpha+ DCs) 
      versus WT C3H (have functional CD8alpha T cells and CD8alpha+ DCs) mice. We also 
      determined whether the phenotype of CD8alpha-/- and BXH2 mice could be restored to 
      that of WT mice by adoptive transfer of WT CD8+ T cells or bone marrow (BM) 
      derived CD8alpha+ DCs. Our results clearly demonstrate that CD8alpha DCs, rather than CD8 
      T cells, are responsible for enhanced viral latency and recurrences.
FAU - Mott, Kevin R
AU  - Mott KR
AD  - Center for Neurobiology and Vaccine Development, Ophthalmology Research, 
      Department of Surgery, Cedars-Sinai Burns & Allen Research Institute, Los 
      Angeles, California, United States of America.
FAU - Allen, Sariah J
AU  - Allen SJ
AD  - Center for Neurobiology and Vaccine Development, Ophthalmology Research, 
      Department of Surgery, Cedars-Sinai Burns & Allen Research Institute, Los 
      Angeles, California, United States of America.
FAU - Zandian, Mandana
AU  - Zandian M
AD  - Center for Neurobiology and Vaccine Development, Ophthalmology Research, 
      Department of Surgery, Cedars-Sinai Burns & Allen Research Institute, Los 
      Angeles, California, United States of America.
FAU - Konda, Bindu
AU  - Konda B
AD  - Departments of Neurosurgery and Biomedical Sciences, Maxine Dunitz Neurosurgical 
      Institute, Cedars-Sinai Medical Center, Los Angeles, California, United States of 
      America.
FAU - Sharifi, Behrooz G
AU  - Sharifi BG
AD  - Division of Cardiology, Department of Medicine, Cedars-Sinai Medical Center, Los 
      Angeles, California, United States of America.
FAU - Jones, Clinton
AU  - Jones C
AD  - School of Veterinary Medicine and Biomedical Sciences, Nebraska Center for 
      Virology, University of Nebraska, Lincoln, Nebraska, United States of America.
FAU - Wechsler, Steven L
AU  - Wechsler SL
AD  - Gavin Herbert Eye Institute, The Department of Ophthalmology, The Department of 
      Microbiology and Molecular Genetics, and the Center for Virus Research, 
      University of California Irvine, School of Medicine, Irvine, California, United 
      States of America.
FAU - Town, Terrence
AU  - Town T
AD  - Zilkha Neurogenetic Institute, Department of Physiology and Biophysics, Keck 
      School of Medicine of the University of Southern California, Los Angeles, 
      California, United States of America.
FAU - Ghiasi, Homayon
AU  - Ghiasi H
AD  - Center for Neurobiology and Vaccine Development, Ophthalmology Research, 
      Department of Surgery, Cedars-Sinai Burns & Allen Research Institute, Los 
      Angeles, California, United States of America.
LA  - eng
GR  - T32 AI89553/AI/NIAID NIH HHS/United States
GR  - R01 EY013615/EY/NEI NIH HHS/United States
GR  - AI093941/AI/NIAID NIH HHS/United States
GR  - EY13615/EY/NEI NIH HHS/United States
GR  - T32 AI089553/AI/NIAID NIH HHS/United States
GR  - R21 AI093941/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140402
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (CD8 Antigens)
RN  - 0 (CD8alpha antigen)
SB  - IM
MH  - Adoptive Transfer/methods
MH  - Animals
MH  - CD8 Antigens/*immunology
MH  - CD8-Positive T-Lymphocytes/immunology/*metabolism/virology
MH  - Dendritic Cells/*immunology/*metabolism/virology
MH  - Herpesvirus 1, Human/*immunology
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Recurrence
MH  - Trigeminal Ganglion/immunology/metabolism/virology
MH  - Virus Latency/*immunology
PMC - PMC3973679
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/04/04 06:00
MHDA- 2015/01/21 06:00
PMCR- 2014/04/02
CRDT- 2014/04/04 06:00
PHST- 2014/02/03 00:00 [received]
PHST- 2014/03/03 00:00 [accepted]
PHST- 2014/04/04 06:00 [entrez]
PHST- 2014/04/04 06:00 [pubmed]
PHST- 2015/01/21 06:00 [medline]
PHST- 2014/04/02 00:00 [pmc-release]
AID - PONE-D-14-04532 [pii]
AID - 10.1371/journal.pone.0093444 [doi]
PST - epublish
SO  - PLoS One. 2014 Apr 2;9(4):e93444. doi: 10.1371/journal.pone.0093444. eCollection 
      2014.