PMID- 2469641
OWN - NLM
STAT- MEDLINE
DCOM- 19890619
LR  - 20181113
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 66
IP  - 4
DP  - 1989 Apr
TI  - An in vitro model of allergen-dependent IgE synthesis by human B lymphocytes: 
      comparison of the response of an atopic and a non-atopic individual to 
      Dermatophagoides spp. (house dust mite).
PG  - 499-504
AB  - An allergen-dependent in vitro model of immunoglobulin E (IgE) synthesis by human 
      B cells is reported. Using this model, it is demonstrated that polyclonal T cells 
      and CD4+ Dermatophagoides spp. (house dust mite)-specific T-cell clones derived 
      from an atopic, house dust mite (HDM)-allergic individual are able to support IgE 
      synthesis by autologous B cells. The helper activity was interleukin-4 (IL-4) 
      dependent as only cloned T cells expressing detectable mRNA for IL-4 were able to 
      induce IgE synthesis without the addition of exogenous IL-4. Peripheral and 
      cloned T cells reactive with HDM could also be identified from a non-atopic 
      individual but neither population was able to support IgE production even in the 
      presence of exogenous IL-4.
FAU - O'Hehir, R E
AU  - O'Hehir RE
AD  - Department of Allergy and Clinical Immunology, National Heart and Lung Institute, 
      Brompton Hospital, Middlesex.
FAU - Bal, V
AU  - Bal V
FAU - Quint, D
AU  - Quint D
FAU - Moqbel, R
AU  - Moqbel R
FAU - Kay, A B
AU  - Kay AB
FAU - Zanders, E D
AU  - Zanders ED
FAU - Lamb, J R
AU  - Lamb JR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Allergens)
RN  - 0 (Dust)
RN  - 0 (Epitopes)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Interleukins)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*immunology
MH  - Animals
MH  - B-Lymphocytes/*immunology/metabolism
MH  - Cells, Cultured
MH  - Dust
MH  - Epitopes/immunology
MH  - Humans
MH  - Immunoglobulin E/*biosynthesis
MH  - Immunoglobulin G/biosynthesis
MH  - Interleukin-4
MH  - Interleukins/immunology
MH  - Mites/*immunology
MH  - Rhinitis, Allergic, Perennial/*immunology
MH  - T-Lymphocytes/immunology
PMC - PMC1385148
EDAT- 1989/04/01 00:00
MHDA- 1989/04/01 00:01
PMCR- 1990/04/01
CRDT- 1989/04/01 00:00
PHST- 1989/04/01 00:00 [pubmed]
PHST- 1989/04/01 00:01 [medline]
PHST- 1989/04/01 00:00 [entrez]
PHST- 1990/04/01 00:00 [pmc-release]
PST - ppublish
SO  - Immunology. 1989 Apr;66(4):499-504.