PMID- 24696423
OWN - NLM
STAT- MEDLINE
DCOM- 20160426
LR  - 20220321
IS  - 2194-9387 (Electronic)
IS  - 2194-9379 (Linking)
VI  - 65
IP  - 7
DP  - 2015 Jul
TI  - Antidepressant Effect of Crocus sativus Aqueous Extract and its Effect on CREB, 
      BDNF, and VGF Transcript and Protein Levels in Rat Hippocampus.
PG  - 337-43
LID - 10.1055/s-0034-1371876 [doi]
AB  - Crocus sativus L., commonly known as saffron, is a perennial stemless herb in 
      Iridaceae family. It has been used in traditional medicine as well as in modern 
      pharmacological studies for variety of conditions including depression. Recent 
      studies have suggested brain-derived neurotrophic factor (BDNF), VGF 
      Neuropeptide, Cyclic-AMP Response Element Binding Protein (CREB) and phospho-CREB 
      (p-CREB) may play roles in depression. In this research the molecular mechanism 
      of antidepressant effect of aqueous extract of saffron and its effect on the 
      levels of BDNF, VGF, CREB and p-CREB in rat hippocampus, were investigated. The 
      aqueous extract of saffron (40, 80 and 160 mg/kg/day) and imipramine 10 mg/kg/day 
      were injected intraperitoneally (i.p.) for 21 days to rats. The FST (forced 
      swimming test) was performed on the days 1(st) and 21(st). The protein expression 
      and transcript levels of BDNF, VGF CREB and phospho-CREB in rat hippocampus, were 
      evaluated using western blot and quantitative reverse transcription-polymerase 
      chain reaction (qRT-PCR). The results of FST showed that saffron reduced the 
      immobility time. The protein levels of BDNF, CREB and p-CREB were significantly 
      increased in saffron treated rats. VGF protein expression was also increased, but 
      not significantly. The transcript levels of BDNF significantly increased. No 
      significant changes in CREB and VGF transcript levels were observed. It was 
      concluded that aqueous extract of saffron has antidepressant effects and the 
      mechanism of its antidepressant effect may be due to increasing the levels of 
      BDNF, VGF, CREB and P-CREB in rat hippocampus.
CI  - (c) Georg Thieme Verlag KG Stuttgart . New York.
FAU - Ghasemi, T
AU  - Ghasemi T
AD  - Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
FAU - Abnous, K
AU  - Abnous K
AD  - Pharmaceutical Research Center, Department of Medicinal Chemistry, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
FAU - Vahdati, F
AU  - Vahdati F
AD  - Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
FAU - Mehri, S
AU  - Mehri S
AD  - Pharmaceutical Research Center, Department of Pharmacodynamy and Toxicology, 
      School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
FAU - Razavi, B M
AU  - Razavi BM
AD  - Targeted Drug Delivery Research Center, Department of Pharmacodynamy and 
      Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, 
      Iran.
FAU - Hosseinzadeh, H
AU  - Hosseinzadeh H
AD  - Pharmaceutical Research Center, Department of Pharmacodynamy and Toxicology, 
      School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140402
PL  - Germany
TA  - Drug Res (Stuttg)
JT  - Drug research
JID - 101602406
RN  - 0 (Antidepressive Agents)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Neuropeptides)
RN  - 0 (Plant Extracts)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vgf protein, rat)
RN  - 7171WSG8A2 (BDNF protein, human)
RN  - OGG85SX4E4 (Imipramine)
SB  - IM
MH  - Animals
MH  - Antidepressive Agents/*pharmacology
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis/genetics
MH  - Crocus/*chemistry
MH  - Cyclic AMP Response Element-Binding Protein/*biosynthesis/genetics
MH  - Dose-Response Relationship, Drug
MH  - Gene Expression/drug effects
MH  - Hippocampus/*drug effects/metabolism
MH  - Imipramine/pharmacology
MH  - Male
MH  - Neuropeptides/*biosynthesis/genetics
MH  - Phosphorylation/drug effects
MH  - Plant Extracts/*pharmacology
MH  - RNA, Messenger/metabolism
MH  - Rats
EDAT- 2014/04/04 06:00
MHDA- 2016/04/27 06:00
CRDT- 2014/04/04 06:00
PHST- 2014/04/04 06:00 [entrez]
PHST- 2014/04/04 06:00 [pubmed]
PHST- 2016/04/27 06:00 [medline]
AID - 10.1055/s-0034-1371876 [doi]
PST - ppublish
SO  - Drug Res (Stuttg). 2015 Jul;65(7):337-43. doi: 10.1055/s-0034-1371876. Epub 2014 
      Apr 2.