PMID- 2469721
OWN - NLM
STAT- MEDLINE
DCOM- 19890619
LR  - 20071114
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 142
IP  - 10
DP  - 1989 May 15
TI  - Polyvalent human immunodeficiency virus synthetic immunogen comprised of envelope 
      gp120 T helper cell sites and B cell neutralization epitopes.
PG  - 3612-9
AB  - In previous studies, we have used antisera raised to envelope (env)-gene-encoded 
      synthetic peptides to identify a region of (HIV) glycoprotein (gp) 120 env 
      protein designated SP10 that contains a type-specific neutralizing determinant. 
      To develop a polyvalent, synthetic peptide inoculum that can evoke both 
      neutralizing antibodies and T cell proliferative responses to more than one HIV 
      isolate, synthetic peptides containing type-specific neutralizing determinants of 
      gp120 from HIV isolates HTLV-IIIB (IIIB), HTLV-IIIMN (MN) and HTLV-IIIRF (RF) 
      were coupled to a 16 amino acid T cell epitope (T1) of HIV-IIIB gp120 and used to 
      immunize goats. Goat antisera to each T1-SP10 peptide derived from the SP10 
      region of gp120 of IIIB, MN, and RF neutralized HIV isolates IIIB, MN and RF in a 
      type-specific manner. Moreover, peripheral blood T cells from immunized goats 
      also proliferated in a type-specific manner to peptides derived from gp120 of 
      IIIB, MN, and RF. When combined in a trivalent inoculum, T1-SP10 peptides from 
      HIV-1 isolates IIIB, MN, and RF evoked a high titered neutralizing antibody 
      response to isolates IIIB, MN, and RF in goats and as well induced immune T cells 
      to undergo blast transformation in the presence of peptides derived from gp120 of 
      all three HIV isolates. The T1 portion of the T1-SP10 construct was shown to 
      induce a B cell antibody response against determinants within the T1 peptide in 
      addition to inducing T cell proliferative responses in immune goat T cells. 
      Moreover, the SP10 portion of the T1-SP10 constructs not only induced B cell 
      antibody production but also induced type-specific T cell proliferative responses 
      localized to the C-terminal variable sequences of the SP10 peptides. Finally, the 
      T1-SP10 peptide construct induced memory T cell proliferative responses to native 
      gp120 env protein. Thus, combinations of homologous SP10 region synthetic 
      peptides containing type-specific neutralizing determinants and T cell epitopes 
      of HIV gp120 may be useful in man to elicit high titered neutralizing B cell 
      responses and, as well, T cell responses to more than one HIV isolate.
FAU - Palker, T J
AU  - Palker TJ
AD  - Department of Medicine, Duke University Medical Center, Durham, NC 27710.
FAU - Matthews, T J
AU  - Matthews TJ
FAU - Langlois, A
AU  - Langlois A
FAU - Tanner, M E
AU  - Tanner ME
FAU - Martin, M E
AU  - Martin ME
FAU - Scearce, R M
AU  - Scearce RM
FAU - Kim, J E
AU  - Kim JE
FAU - Berzofsky, J A
AU  - Berzofsky JA
FAU - Bolognesi, D P
AU  - Bolognesi DP
FAU - Haynes, B F
AU  - Haynes BF
LA  - eng
GR  - P01 CA43447/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Epitopes)
RN  - 0 (HIV Envelope Protein gp120)
RN  - 0 (Immune Sera)
RN  - 0 (Peptide Fragments)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Retroviridae Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Viral/biosynthesis
MH  - Antigens, Viral/analysis/*immunology
MH  - B-Lymphocytes/*immunology/metabolism
MH  - Epitopes/analysis/immunology
MH  - Goats
MH  - HIV/*immunology/isolation & purification
MH  - HIV Envelope Protein gp120
MH  - Immune Sera
MH  - Immunologic Memory
MH  - Lymphocyte Activation
MH  - Mice
MH  - Molecular Sequence Data
MH  - Neutralization Tests
MH  - Peptide Fragments/chemical synthesis/immunology
MH  - Recombinant Proteins/analysis/*immunology
MH  - Retroviridae Proteins/analysis/*immunology
MH  - T-Lymphocytes, Helper-Inducer/*immunology
EDAT- 1989/05/15 00:00
MHDA- 1989/05/15 00:01
CRDT- 1989/05/15 00:00
PHST- 1989/05/15 00:00 [pubmed]
PHST- 1989/05/15 00:01 [medline]
PHST- 1989/05/15 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1989 May 15;142(10):3612-9.