PMID- 24698304
OWN - NLM
STAT- MEDLINE
DCOM- 20150722
LR  - 20141203
IS  - 1469-0691 (Electronic)
IS  - 1198-743X (Linking)
VI  - 20
IP  - 10
DP  - 2014 Oct
TI  - Detection of HHV-6-specific mRNA and antigens in PBMCs of individuals with 
      chromosomally integrated HHV-6 (ciHHV-6).
PG  - 1027-32
LID - 10.1111/1469-0691.12639 [doi]
AB  - After inheritance of chromosomally integrated HHV-6 (ciHHV-6), viral DNA is found 
      in every nucleated cell. The prevalence of ciHHV-6 is estimated to be 0.2-5% of 
      humans. There are conflicting data on the potential for replication, possibly 
      leading to clinical implications. We analysed peripheral blood mononuclear cells 
      (PBMCs) from individuals with ciHHV-6 proven by fluorescence in situ 
      hybridization (FISH) for HHV-6-specific mRNA (U94, U42, U22) and antigens by 
      means of reverse transcription PCR and an indirect immunoperoxidase staining. U94 
      transcripts indicative of latent infection were detected in six (54.5%) out of 11 
      individuals at least once. Transcripts indicative of lytic infection (i.e. U42 
      and U22) were detected in four (36.4%) out of 11 individuals at least once. HHV-6 
      antigen was detected in seven (70%) out of 10 individuals at least once. The 
      presence of viral mRNA and proteins supports virus gene expression from ciHHV-6, 
      which may lead to virus replication. Considering the properties of active HHV-6 
      infection together with obvious replicative activity in individuals with ciHHV-6, 
      pathophysiological effects leading to clinical consequences of chromosomally 
      integrated viral DNA might be considered.
CI  - (c) 2014 The Authors Clinical Microbiology and Infection (c) 2014 European Society of 
      Clinical Microbiology and Infectious Diseases.
FAU - Strenger, V
AU  - Strenger V
AD  - Department of Pediatrics and Adolescent Medicine, Medical University of Graz, 
      Graz, Austria.
FAU - Caselli, E
AU  - Caselli E
FAU - Lautenschlager, I
AU  - Lautenschlager I
FAU - Schwinger, W
AU  - Schwinger W
FAU - Aberle, S W
AU  - Aberle SW
FAU - Loginov, R
AU  - Loginov R
FAU - Gentili, V
AU  - Gentili V
FAU - Nacheva, E
AU  - Nacheva E
FAU - DiLuca, D
AU  - DiLuca D
FAU - Urban, C
AU  - Urban C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140515
PL  - England
TA  - Clin Microbiol Infect
JT  - Clinical microbiology and infection : the official publication of the European 
      Society of Clinical Microbiology and Infectious Diseases
JID - 9516420
RN  - 0 (Antigens, Viral)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Antigens, Viral/*genetics/metabolism
MH  - Child
MH  - Chromosomes, Human/*virology
MH  - Female
MH  - Herpesvirus 6, Human/genetics/*immunology
MH  - Humans
MH  - Leukocytes, Mononuclear/*virology
MH  - Male
MH  - Middle Aged
MH  - Molecular Diagnostic Techniques/*methods
MH  - RNA, Messenger/*genetics
MH  - RNA, Viral/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Roseolovirus Infections/blood/*diagnosis/virology
MH  - Sensitivity and Specificity
MH  - Virus Integration
MH  - Young Adult
OTO - NOTNLM
OT  - Chromosomal integration
OT  - HHV-6
OT  - herpesvirus
OT  - lytic infection
OT  - mRNA
OT  - viral antigen
OT  - viral replication
EDAT- 2014/04/05 06:00
MHDA- 2015/07/23 06:00
CRDT- 2014/04/05 06:00
PHST- 2014/01/08 00:00 [received]
PHST- 2014/03/29 00:00 [revised]
PHST- 2014/03/31 00:00 [accepted]
PHST- 2014/04/05 06:00 [entrez]
PHST- 2014/04/05 06:00 [pubmed]
PHST- 2015/07/23 06:00 [medline]
AID - S1198-743X(14)65373-8 [pii]
AID - 10.1111/1469-0691.12639 [doi]
PST - ppublish
SO  - Clin Microbiol Infect. 2014 Oct;20(10):1027-32. doi: 10.1111/1469-0691.12639. 
      Epub 2014 May 15.