PMID- 24699060
OWN - NLM
STAT- MEDLINE
DCOM- 20150609
LR  - 20140923
IS  - 1461-7285 (Electronic)
IS  - 0269-8811 (Linking)
VI  - 28
IP  - 10
DP  - 2014 Oct
TI  - Effects of lithium and valproic acid on BDNF protein and gene expression in an in 
      vitro human neuron-like model of degeneration.
PG  - 964-72
LID - 10.1177/0269881114529379 [doi]
AB  - One of the common effects of lithium (Li) and valproic acid (VPA) is their 
      ability to protect against excitotoxic insults. Neurodegenerative and 
      neuropsychiatric diseases may be also associated with altered trophic support of 
      brain-derived neurotrophic factor (BDNF), the most widely distributed 
      neurotrophin in the central nervous system. However, despite these evidences, the 
      effect of Li-VPA combination on BDNF after excitoxic insult has been inadequately 
      investigated. We address this issue by exposing a human neuroblastoma cell line 
      (SH-SY5Y) to neurotoxic concentration of L-glutamate and exploring whether the 
      neuroprotective action of Li-VPA on these cells is associated with changes in 
      BDNF protein and mRNA levels. The results showed that pre-incubation of Li-VPA 
      abolished the toxic effect of glutamate on SH-SY5Y cell survival and this 
      neuroprotective effect was associated with increased synthesis and mRNA 
      expression of BDNF after 24 and 48 h of incubation. In conclusion, this study 
      demonstrates that the neuroprotective effects of Li-VPA against glutamate-induced 
      neurotoxicity in SH-SY5Y neuroblastoma cells is associated with increased 
      synthesis and mRNA expression of BDNF. These data further support the idea that 
      these two drugs can be used for prevention and/or treatment of glutamate-related 
      neurodegenerative disorders.
CI  - (c) The Author(s) 2014.
FAU - Croce, Nicoletta
AU  - Croce N
AD  - IRCCS Santa Lucia Foundation, Rome, Italy Department of Internal Medicine, Tor 
      Vergata University, Rome, Italy.
FAU - Mathe, Aleksander A
AU  - Mathe AA
AD  - Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden.
FAU - Gelfo, Francesca
AU  - Gelfo F
AD  - IRCCS Santa Lucia Foundation, Rome, Italy Department of Systemic Medicine, Tor 
      Vergata University, Rome, Italy.
FAU - Caltagirone, Carlo
AU  - Caltagirone C
AD  - IRCCS Santa Lucia Foundation, Rome, Italy Department of Systemic Medicine, Tor 
      Vergata University, Rome, Italy.
FAU - Bernardini, Sergio
AU  - Bernardini S
AD  - Department of Internal Medicine, Tor Vergata University, Rome, Italy.
FAU - Angelucci, Francesco
AU  - Angelucci F
AD  - IRCCS Santa Lucia Foundation, Rome, Italy f.angelucci@hsantalucia.it.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140402
PL  - United States
TA  - J Psychopharmacol
JT  - Journal of psychopharmacology (Oxford, England)
JID - 8907828
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neuroprotective Agents)
RN  - 2BMD2GNA4V (Lithium Carbonate)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 614OI1Z5WI (Valproic Acid)
SB  - IM
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis/*genetics
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Drug Synergism
MH  - Gene Expression Regulation/*drug effects
MH  - Glutamic Acid/toxicity
MH  - Humans
MH  - Lithium Carbonate/*pharmacology
MH  - Neurons/drug effects/metabolism
MH  - Neuroprotective Agents/*pharmacology
MH  - Valproic Acid/*pharmacology
OTO - NOTNLM
OT  - BDNF
OT  - L-glutamate
OT  - Lithium
OT  - SH-SY5Y
OT  - excitotoxicity
OT  - neuronal survival
OT  - valproic acid
EDAT- 2014/04/05 06:00
MHDA- 2015/06/10 06:00
CRDT- 2014/04/05 06:00
PHST- 2014/04/05 06:00 [entrez]
PHST- 2014/04/05 06:00 [pubmed]
PHST- 2015/06/10 06:00 [medline]
AID - 0269881114529379 [pii]
AID - 10.1177/0269881114529379 [doi]
PST - ppublish
SO  - J Psychopharmacol. 2014 Oct;28(10):964-72. doi: 10.1177/0269881114529379. Epub 
      2014 Apr 2.