PMID- 24700142
OWN - NLM
STAT- MEDLINE
DCOM- 20141217
LR  - 20211203
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 31
IP  - 6
DP  - 2014 Jun
TI  - SOX2 promotes the migration and invasion of laryngeal cancer cells by induction 
      of MMP-2 via the PI3K/Akt/mTOR pathway.
PG  - 2651-9
LID - 10.3892/or.2014.3120 [doi]
AB  - SOX2 is a high mobility group box containing transcription factor that has been 
      reported to be aberrantly overexpressed in various human malignancies, including 
      laryngeal squamous cell carcinoma (LSCC). However, the potential role of SOX2 in 
      LSCC migration and invasion remains to be elucidated. In the present study, we 
      generated stable transformants of human LSCC cells constitutively overexpressing 
      SOX2 and investigated the effects of SOX2 overexpression on migration and 
      invasion in LSCC cells as well as the possible underlying mechanisms. We found 
      that ectopic overexpression of SOX2 in LSCC cells enhanced their migratory and 
      invasive ability in vitro, accompanied by increased expression and activity of 
      matrix metalloproteinase (MMP)-2. Meanwhile, SOX2-induced cell migration and 
      invasion were significantly abrogated by a neutralizing anti-MMP-2 antibody or 
      small interfering RNA targeting MMP-2. Furthermore, overexpression of SOX2 
      induced phosphorylation of Akt and mammalian target of rapamycin (mTOR), which 
      are downstream effectors of the PI3K pathway. Finally, LY294002, an inhibitor of 
      PI3K, also markedly abolished SOX2-induced activation of the Akt/mTOR pathway and 
      increased cell invasion and MMP-2 expression. Taken together, we conclude that 
      SOX2 promotes migration and invasion of laryngeal cancer cells by inducing MMP-2 
      via the PI3K/Akt/mTOR pathway. Our findings suggest that SOX2 may serve as a 
      potential therapeutic target for LSCC.
FAU - Yang, Ning
AU  - Yang N
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of China Medical 
      University, Shenyang 110001, P.R. China.
FAU - Hui, Lian
AU  - Hui L
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of China Medical 
      University, Shenyang 110001, P.R. China.
FAU - Wang, Yan
AU  - Wang Y
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of China Medical 
      University, Shenyang 110001, P.R. China.
FAU - Yang, Huijun
AU  - Yang H
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of China Medical 
      University, Shenyang 110001, P.R. China.
FAU - Jiang, Xuejun
AU  - Jiang X
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of China Medical 
      University, Shenyang 110001, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140402
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (SOX2 protein, human)
RN  - 0 (SOXB1 Transcription Factors)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (Oncogene Protein v-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
SB  - IM
MH  - Cell Movement/*genetics
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Hep G2 Cells
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Matrix Metalloproteinase 2/*genetics/metabolism
MH  - Matrix Metalloproteinase Inhibitors
MH  - Neoplasm Invasiveness/genetics
MH  - Oncogene Protein v-akt/genetics/metabolism
MH  - Phosphatidylinositol 3-Kinases/genetics/metabolism
MH  - SOXB1 Transcription Factors/*biosynthesis/genetics
MH  - TOR Serine-Threonine Kinases/genetics/metabolism
EDAT- 2014/04/05 06:00
MHDA- 2014/12/18 06:00
CRDT- 2014/04/05 06:00
PHST- 2014/02/14 00:00 [received]
PHST- 2014/03/12 00:00 [accepted]
PHST- 2014/04/05 06:00 [entrez]
PHST- 2014/04/05 06:00 [pubmed]
PHST- 2014/12/18 06:00 [medline]
AID - 10.3892/or.2014.3120 [doi]
PST - ppublish
SO  - Oncol Rep. 2014 Jun;31(6):2651-9. doi: 10.3892/or.2014.3120. Epub 2014 Apr 2.