PMID- 24706283
OWN - NLM
STAT- MEDLINE
DCOM- 20141117
LR  - 20140407
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1142
DP  - 2014
TI  - Sample processing, protocol, and statistical analysis of the time-of-flight 
      secondary ion mass spectrometry (ToF-SIMS) of protein, cell, and tissue samples.
PG  - 177-88
LID - 10.1007/978-1-4939-0404-4_15 [doi]
AB  - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is increasingly being 
      used in analysis of biological samples. For example, it has been applied to 
      distinguish healthy and osteoarthritic human cartilage. This chapter discusses 
      ToF-SIMS principle and instrumentation including the three modes of analysis in 
      ToF-SIMS. ToF-SIMS sets certain requirements for the samples to be analyzed; for 
      example, the samples have to be vacuum compatible. Accordingly, sample processing 
      steps for different biological samples, i.e., proteins, cells, frozen and 
      paraffin-embedded tissues and extracellular matrix for the ToF-SIMS are 
      presented. Multivariate analysis of the ToF-SIMS data and the necessary data 
      preprocessing steps (peak selection, data normalization, mean-centering, and 
      scaling and transformation) are discussed in this chapter.
FAU - Barreto, Goncalo
AU  - Barreto G
AD  - Institute of Clinical Medicine, University of Helsinki, Haartmaninkatu 8, 
      Biomedicum 1, P.O. Box 700, 00029 HUS, Helsinki, Finland.
FAU - Soininen, Antti
AU  - Soininen A
FAU - Sillat, Tarvo
AU  - Sillat T
FAU - Konttinen, Yrjo T
AU  - Konttinen YT
FAU - Kaivosoja, Emilia
AU  - Kaivosoja E
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Proteins)
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques
MH  - Frozen Sections
MH  - Humans
MH  - Multivariate Analysis
MH  - Paraffin Embedding
MH  - Principal Component Analysis
MH  - Proteins/chemistry
MH  - Spectrometry, Mass, Secondary Ion/*methods
MH  - Tissue Fixation
EDAT- 2014/04/08 06:00
MHDA- 2014/11/18 06:00
CRDT- 2014/04/08 06:00
PHST- 2014/04/08 06:00 [entrez]
PHST- 2014/04/08 06:00 [pubmed]
PHST- 2014/11/18 06:00 [medline]
AID - 10.1007/978-1-4939-0404-4_15 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2014;1142:177-88. doi: 10.1007/978-1-4939-0404-4_15.