PMID- 24706756
OWN - NLM
STAT- MEDLINE
DCOM- 20141028
LR  - 20211021
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 289
IP  - 21
DP  - 2014 May 23
TI  - Homeobox transcription factor VentX regulates differentiation and maturation of 
      human dendritic cells.
PG  - 14633-43
LID - 10.1074/jbc.M113.509158 [doi]
AB  - Dendritic cells (DCs) are specialized antigen presentation cells that play 
      critical roles in the initiation and regulation of immune responses. The 
      molecular determinants of DC differentiation and maturation are target of 
      extensive investigation. VentX is a human homeobox transcriptional factor that 
      regulates proliferation and differentiation of hematopoietic cells. In the 
      current study, we report that ablation of VentX expression in monocytes 
      significantly impaired their differentiation into DCs. Conversely, overexpression 
      of VentX in monocytic THP1 cells accelerated their differentiation toward DCs. We 
      showed that VentX regulates DC differentiation, in part, through modulating IL6 
      expression. Clinically, we found that VentX expression was elevated in intestinal 
      lamina propria DCs (LPDCs) of inflamed mucosa from inflammatory bowel disease 
      patients. Knockdown experiments suggested that VentX is essential for the 
      maturation of LPDCs. In addition, corticosteroid treatment markedly decreased 
      VentX expression in LPDCs and enforced expression of VentX counteracted the 
      effects of corticosteroid on DCs maturation. Our data suggest that VentX is a 
      critical transcriptional regulator of DC differentiation and maturation, and a 
      potential target of immune regulation and therapy.
CI  - (c) 2014 by The American Society for Biochemistry and Molecular Biology, Inc.
FAU - Wu, Xiaoming
AU  - Wu X
AD  - From the Departments of Medicine, Gastroenterology Division and.
FAU - Gao, Hong
AU  - Gao H
AD  - the Department of Medicine, Tufts Medical Center, Boston, Massachusetts 02111.
FAU - Bleday, Ronald
AU  - Bleday R
AD  - Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, 
      Massachusetts 02115 and.
FAU - Zhu, Zhenglun
AU  - Zhu Z
AD  - From the Departments of Medicine, Gastroenterology Division and 
      zzhu@partners.org.
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140404
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Glucocorticoids)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (VENTX protein, human)
RN  - 9PHQ9Y1OLM (Prednisolone)
SB  - IM
MH  - Adult
MH  - Blotting, Western
MH  - Cell Differentiation/drug effects/*genetics
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cells, Cultured
MH  - Dendritic Cells/drug effects/*metabolism
MH  - Gene Expression/drug effects
MH  - Gene Knockdown Techniques
MH  - Glucocorticoids/pharmacology
MH  - Homeodomain Proteins/*genetics/metabolism
MH  - Humans
MH  - Inflammatory Bowel Diseases/genetics/metabolism
MH  - Interleukin-6/genetics/metabolism
MH  - Intestinal Mucosa/metabolism/pathology
MH  - Lipopolysaccharides/pharmacology
MH  - Monocytes/*metabolism
MH  - Prednisolone/pharmacology
MH  - Reverse Transcriptase Polymerase Chain Reaction
PMC - PMC4031519
OTO - NOTNLM
OT  - Dendritic Cells
OT  - Development
OT  - Differentiation
OT  - Gene Regulation
OT  - Monocytes
EDAT- 2014/04/08 06:00
MHDA- 2014/10/29 06:00
PMCR- 2015/05/23
CRDT- 2014/04/08 06:00
PHST- 2014/04/08 06:00 [entrez]
PHST- 2014/04/08 06:00 [pubmed]
PHST- 2014/10/29 06:00 [medline]
PHST- 2015/05/23 00:00 [pmc-release]
AID - S0021-9258(20)38667-1 [pii]
AID - M113.509158 [pii]
AID - 10.1074/jbc.M113.509158 [doi]
PST - ppublish
SO  - J Biol Chem. 2014 May 23;289(21):14633-43. doi: 10.1074/jbc.M113.509158. Epub 
      2014 Apr 4.