PMID- 2470780
OWN - NLM
STAT- MEDLINE
DCOM- 19890626
LR  - 20190824
IS  - 0165-5728 (Print)
IS  - 0165-5728 (Linking)
VI  - 23
IP  - 1
DP  - 1989 Jun
TI  - Interferon-beta impairs induction of HLA-DR antigen expression in cultured adult 
      human astrocytes.
PG  - 45-53
AB  - We studied the effect of interferons on the expression of class II 
      histocompatibility (HLA-DR) antigens by cultured adult human astrocytes. Cultures 
      were derived from brain tissue resected for surgical treatment of intractable 
      epilepsy. Cultured astrocytes did not spontaneously display HLA-DR antigen as 
      determined by immunocytochemistry and flow cytometry with antibody to HLA-DR. 
      Astrocytes cultured for 72 h with recombinant or natural interferon-gamma (IFN 
      gamma) demonstrated a dose-dependent increase in HLA-DR expression with optimal 
      stimulation by 100 U/ml IFN gamma. HLA-DR expression was not detectable in 
      astrocytes cultured with IFN gamma for less then 48 h, and peak HLA-DR expression 
      (over 80% of cells) was seen at 120 h of culture. Optimal HLA-DR expression 
      required continuous presence of IFN gamma. Exposure of astrocytes to recombinant 
      or natural interferon-beta (IFN beta) did not induce HLA-DR and pretreatment of 
      astrocytes with IFN beta or interferon-alpha (IFN alpha) significantly inhibited 
      subsequent induction of HLA-DR expression by IFN gamma. These observations 
      suggest that interferons may function in regulating human astrocyte HLA-DR 
      expression within the central nervous system.
FAU - Barna, B P
AU  - Barna BP
AD  - Department of Immunopathology, Cleveland Clinic Foundation, OH 44195-5131.
FAU - Chou, S M
AU  - Chou SM
FAU - Jacobs, B
AU  - Jacobs B
FAU - Yen-Lieberman, B
AU  - Yen-Lieberman B
FAU - Ransohoff, R M
AU  - Ransohoff RM
LA  - eng
GR  - 1K08 NS01265-01 NSP-A AD/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (Culture Media)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Interferon Type I)
RN  - 0 (Lymphokines)
RN  - 0 (concanavalin A-induced helper factors)
RN  - 11028-71-0 (Concanavalin A)
SB  - IM
MH  - Adult
MH  - Astrocytes/classification/drug effects/*immunology
MH  - Cells, Cultured
MH  - Concanavalin A
MH  - Culture Media
MH  - Dose-Response Relationship, Immunologic
MH  - Flow Cytometry
MH  - HLA-DR Antigens/*biosynthesis
MH  - Humans
MH  - Interferon Type I/*pharmacology
MH  - Kinetics
MH  - Lymphokines/physiology
MH  - Phenotype
MH  - Staining and Labeling
EDAT- 1989/06/01 00:00
MHDA- 2000/06/01 09:00
CRDT- 1989/06/01 00:00
PHST- 1989/06/01 00:00 [pubmed]
PHST- 2000/06/01 09:00 [medline]
PHST- 1989/06/01 00:00 [entrez]
AID - 0165-5728(89)90072-6 [pii]
AID - 10.1016/0165-5728(89)90072-6 [doi]
PST - ppublish
SO  - J Neuroimmunol. 1989 Jun;23(1):45-53. doi: 10.1016/0165-5728(89)90072-6.