PMID- 24708411
OWN - NLM
STAT- MEDLINE
DCOM- 20150303
LR  - 20211021
IS  - 1365-2222 (Electronic)
IS  - 0954-7894 (Print)
IS  - 0954-7894 (Linking)
VI  - 44
IP  - 7
DP  - 2014 Jul
TI  - Grass-specific CD4(+) T-cells exhibit varying degrees of cross-reactivity, 
      implications for allergen-specific immunotherapy.
PG  - 986-98
LID - 10.1111/cea.12324 [doi]
AB  - BACKGROUND: Conceptually, allergic responses may involve cross-reactivity by 
      antibodies or T-cells. While IgE cross-reactivity among grass-pollen allergens 
      has been observed, cross-reactivity at the allergen-specific T-cell level has 
      been less documented. Identification of the patterns of cross-reactivity may 
      improve our understanding, allowing optimization of better immunotherapy 
      strategies. OBJECTIVES: We use Phleum pratense as model for the studying of 
      cross-reactivity at the allergen-specific CD4(+) T cell level among DR04:01 
      restricted Pooideae grass-pollen T-cell epitopes. METHODS: After in vitro culture 
      of blood mono-nucleated cells from grass-pollen-allergic subjects with specific 
      Pooideae antigenic epitopes, dual tetramer staining with APC-labelled 
      DR04:01/Phleum pratense tetramers and PE-labelled DR04:01/Pooideae grass homolog 
      tetramers was assessed to identify cross-reactivity among allergen-specific 
      DR04:01-restricted T-cells in six subjects. Direct ex vivo staining enabled the 
      comparison of frequency and phenotype of different Pooideae grass-pollen reactive 
      T-cells. Intracellular cytokine staining (ICS) assays were also used to examine 
      phenotypes of these T-cells. RESULTS: T-cells with various degrees of 
      cross-reactive profiles could be detected. Poa p 1 97-116 , Lol p 1 221-240 , Lol 
      p 5a 199-218 , and Poa p 5a 199-218 were identified as minimally cross-reactive 
      T-cell epitopes that do not show cross-reactivity to Phl p 1 and Phl p 5a 
      epitopes. Ex vivo tetramer staining assays demonstrated T-cells that recognized 
      these minimally cross-reactive T-cell epitopes are present in 
      Grass-pollen-allergic subjects. CONCLUSIONS: Our results suggest that not all 
      Pooideae grass epitopes with sequence homology are cross-reactive. 
      Non-cross-reactive T-cells with comparable frequency, phenotype and functionality 
      to Phl p-specific T-cells suggest that a multiple allergen system should be 
      considered for immunotherapy instead of a mono-allergen system.
CI  - (c) 2014 John Wiley & Sons Ltd.
FAU - Archila, L D
AU  - Archila LD
AD  - Benaroya Research Institute at Virginia Mason, Seattle, WA, USA.
FAU - DeLong, J H
AU  - DeLong JH
FAU - Wambre, E
AU  - Wambre E
FAU - James, E A
AU  - James EA
FAU - Robinson, D M
AU  - Robinson DM
FAU - Kwok, W W
AU  - Kwok WW
LA  - eng
GR  - HHSN272200700046C/AI/NIAID NIH HHS/United States
GR  - R01 AI095074/AI/NIAID NIH HHS/United States
GR  - 5 R01 AI095074/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (Cytokines)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA Antigens)
RN  - 0 (Peptides)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
CIN - Clin Exp Allergy. 2014 Jul;44(7):898-900. PMID: 24953633
MH  - Alleles
MH  - Allergens/*immunology
MH  - Amino Acid Sequence
MH  - Antigens, Plant/chemistry/immunology
MH  - Basophils/immunology/metabolism
MH  - CD4-Positive T-Lymphocytes/*immunology/metabolism
MH  - Cross Reactions/*immunology
MH  - Cytokines/metabolism
MH  - *Desensitization, Immunologic
MH  - Epitopes, T-Lymphocyte/chemistry/immunology
MH  - HLA Antigens/genetics/immunology
MH  - Humans
MH  - Immunoglobulin E/immunology
MH  - Lymphocyte Activation/immunology
MH  - Peptides/chemistry/immunology
MH  - Poaceae/*immunology
MH  - Pollen/immunology
MH  - T-Cell Antigen Receptor Specificity/immunology
MH  - T-Lymphocyte Subsets/*immunology/metabolism
PMC - PMC4069242
MID - NIHMS583566
OTO - NOTNLM
OT  - CD4+
OT  - MHC class II tetramers
OT  - Pooideae
OT  - T-cells
OT  - allergy
OT  - cross-reactivity
OT  - epitopes
OT  - grass-pollen
EDAT- 2014/04/09 06:00
MHDA- 2015/03/04 06:00
PMCR- 2015/07/01
CRDT- 2014/04/09 06:00
PHST- 2013/12/03 00:00 [received]
PHST- 2014/03/07 00:00 [revised]
PHST- 2014/03/21 00:00 [accepted]
PHST- 2014/04/09 06:00 [entrez]
PHST- 2014/04/09 06:00 [pubmed]
PHST- 2015/03/04 06:00 [medline]
PHST- 2015/07/01 00:00 [pmc-release]
AID - 10.1111/cea.12324 [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2014 Jul;44(7):986-98. doi: 10.1111/cea.12324.