PMID- 24709112
OWN - NLM
STAT- MEDLINE
DCOM- 20140912
LR  - 20140614
IS  - 1521-7035 (Electronic)
IS  - 1521-6616 (Linking)
VI  - 153
IP  - 1
DP  - 2014 Jul
TI  - Decreased dendritic cell numbers but increased TLR9-mediated interferon-alpha 
      production in first degree relatives of type 1 diabetes patients.
PG  - 49-55
LID - S1521-6616(14)00082-5 [pii]
LID - 10.1016/j.clim.2014.03.018 [doi]
AB  - OBJECTIVE: Dendritic cells (DCs) play an important role in pathogenesis of 
      autoimmunity, including type 1 diabetes (T1D). In this study, we investigated DC 
      subpopulations and their responses to TLR stimulation in T1D patients and their 
      relatives. METHODS: We analyzed the frequency of myeloid (mDCs) and plasmacytoid 
      DCs (pDCs) in 97 T1D patients (69 onset, 28 long-term), 67 first-degree 
      relatives, and 64 controls. We additionally tested the IFN-alpha production by 
      pDCs upon stimulation with TLR 7, 8 and 9 agonists. RESULTS: A lower number of 
      mDCs and pDCs were found in T1D patients and their relatives. Of all the tested 
      TLR ligands, only stimulation with CpG 2216 induced IFN-alpha production that was 
      the highest in T1D relatives, except of autoantibody-negative relatives bearing 
      the protective haplotypes. CONCLUSION: Our data demonstrate disturbances in DC 
      number and function expressed most significantly in T1D relatives and point to a 
      potential role of TLR9-induced IFN-alpha production in T1D development.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Kayserova, Jana
AU  - Kayserova J
AD  - Department of Immunology, Charles University, 2nd Faculty of Medicine, University 
      Hospital Motol, V Uvalu 84, Prague, Czech Republic. Electronic address: 
      jana.kayserova@lfmotol.cuni.cz.
FAU - Vcelakova, Jana
AU  - Vcelakova J
AD  - Department of Pediatrics, Charles University, 2nd Faculty of Medicine, University 
      Hospital Motol, V Uvalu 84, Prague, Czech Republic. Electronic address: 
      jana.vcelakova@lfmotol.cuni.cz.
FAU - Stechova, Katerina
AU  - Stechova K
AD  - Department of Pediatrics, Charles University, 2nd Faculty of Medicine, University 
      Hospital Motol, V Uvalu 84, Prague, Czech Republic. Electronic address: 
      katerina.stechova@lfmotol.cuni.cz.
FAU - Dudkova, Eva
AU  - Dudkova E
AD  - Department of Immunology, Charles University, 2nd Faculty of Medicine, University 
      Hospital Motol, V Uvalu 84, Prague, Czech Republic. Electronic address: 
      eva.dudkova@fnmotol.cz.
FAU - Hromadkova, Hana
AU  - Hromadkova H
AD  - Department of Immunology, Charles University, 2nd Faculty of Medicine, University 
      Hospital Motol, V Uvalu 84, Prague, Czech Republic. Electronic address: 
      hana.hromadkova@lfmotol.cuni.cz.
FAU - Sumnik, Zdenek
AU  - Sumnik Z
AD  - Department of Pediatrics, Charles University, 2nd Faculty of Medicine, University 
      Hospital Motol, V Uvalu 84, Prague, Czech Republic. Electronic address: 
      zdenek.sumnik@lfmotol.cuni.cz.
FAU - Kolouskova, Stanislava
AU  - Kolouskova S
AD  - Department of Pediatrics, Charles University, 2nd Faculty of Medicine, University 
      Hospital Motol, V Uvalu 84, Prague, Czech Republic. Electronic address: 
      stanislava.kolouskova@lfmotol.cuni.cz.
FAU - Spisek, Radek
AU  - Spisek R
AD  - Department of Immunology, Charles University, 2nd Faculty of Medicine, University 
      Hospital Motol, V Uvalu 84, Prague, Czech Republic; Sotio a.s., Jankovcova 2, 
      Prague, Czech Republic. Electronic address: spisek@sotio.com.
FAU - Sediva, Anna
AU  - Sediva A
AD  - Department of Immunology, Charles University, 2nd Faculty of Medicine, University 
      Hospital Motol, V Uvalu 84, Prague, Czech Republic. Electronic address: 
      anna.sediva@fnmotol.cz.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140405
PL  - United States
TA  - Clin Immunol
JT  - Clinical immunology (Orlando, Fla.)
JID - 100883537
RN  - 0 (CpG ODN 2216)
RN  - 0 (Interferon-alpha)
RN  - 0 (Ligands)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Toll-Like Receptor 9)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Cell Count
MH  - Child
MH  - Child, Preschool
MH  - Dendritic Cells/*immunology/*metabolism
MH  - Diabetes Mellitus, Type 1/*immunology/*metabolism
MH  - Family
MH  - Female
MH  - Humans
MH  - Interferon-alpha/*biosynthesis/blood
MH  - Leukocytes, Mononuclear/drug effects/metabolism
MH  - Ligands
MH  - Male
MH  - Oligodeoxyribonucleotides/pharmacology
MH  - Toll-Like Receptor 9/*metabolism
MH  - Young Adult
OTO - NOTNLM
OT  - Dendritic cell;
OT  - Interferon-alpha;
OT  - Type 1 diabetes
EDAT- 2014/04/09 06:00
MHDA- 2014/09/13 06:00
CRDT- 2014/04/09 06:00
PHST- 2013/09/27 00:00 [received]
PHST- 2014/03/12 00:00 [revised]
PHST- 2014/03/26 00:00 [accepted]
PHST- 2014/04/09 06:00 [entrez]
PHST- 2014/04/09 06:00 [pubmed]
PHST- 2014/09/13 06:00 [medline]
AID - S1521-6616(14)00082-5 [pii]
AID - 10.1016/j.clim.2014.03.018 [doi]
PST - ppublish
SO  - Clin Immunol. 2014 Jul;153(1):49-55. doi: 10.1016/j.clim.2014.03.018. Epub 2014 
      Apr 5.