PMID- 24709620 OWN - NLM STAT- MEDLINE DCOM- 20141218 LR - 20211021 IS - 1873-507X (Electronic) IS - 0031-9384 (Print) IS - 0031-9384 (Linking) VI - 130 DP - 2014 May 10 TI - Effects of energy status and diet on Bdnf expression in the ventromedial hypothalamus of male and female rats. PG - 99-107 LID - S0031-9384(14)00174-7 [pii] LID - 10.1016/j.physbeh.2014.03.028 [doi] AB - Sex differences exist in the regulation of energy homeostasis in response to calorie scarcity or excess. Brain-derived neurotrophic factor (BDNF) is one of the anorexigenic neuropeptides regulating energy homeostasis. Expression of Bdnf mRNA in the ventromedial nucleus of the hypothalamus (VMH) is closely associated with energy and reproductive status. We hypothesized that Bdnf expression in the VMH was differentially regulated by altered energy balance in male and female rats. Using dietary intervention, including fasting-induced negative energy status and high-fat diet (HFD) feeding-induced positive energy status, along with low-fat diet (LFD) feeding and HFD pair-feeding (HFD-PF), effects of diets and changes in energy status on VMH Bdnf expression were compared between male and female rats. Fasted males but not females had lower VMH Bdnf expression than their fed counterparts following 24-hour fasting, suggesting that fasted males reduced Bdnf expression to drive hyperphagia and body weight gain. Male HFD obese and HFD-PF non-obese rats had similarly reduced expression of Bdnf compared with LFD males, indicating that dampened Bdnf expression was associated with feeding a diet high in fat instead of increased adiposity. Decreased BDNF signaling during HFD feeding would increase a drive to eat and may contribute to diet-induced obesity in males. In contrast, VMH Bdnf expression was stably maintained in females when energy homeostasis was disturbed. These results suggest sex-distinct regulation of central Bdnf expression by diet and energy status. CI - Copyright (c) 2014 Elsevier Inc. All rights reserved. FAU - Liu, Xian AU - Liu X AD - Cell, Molecular, and Structural Biology, Miami University, OH, United States. FAU - Zhu, Zheng AU - Zhu Z AD - Physiology and Neuroscience, Department of Biology, Miami University, OH, United States; Department of Statistics, Miami University, OH, United States. FAU - Kalyani, Manu AU - Kalyani M AD - Physiology and Neuroscience, Department of Biology, Miami University, OH, United States. FAU - Janik, James M AU - Janik JM AD - Cell, Molecular, and Structural Biology, Miami University, OH, United States; Physiology and Neuroscience, Department of Biology, Miami University, OH, United States. FAU - Shi, Haifei AU - Shi H AD - Cell, Molecular, and Structural Biology, Miami University, OH, United States; Physiology and Neuroscience, Department of Biology, Miami University, OH, United States. Electronic address: shih@miamioh.edu. LA - eng GR - R15 DK090823/DK/NIDDK NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20140404 PL - United States TA - Physiol Behav JT - Physiology & behavior JID - 0151504 RN - 0 (Blood Glucose) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Leptin) RN - 0 (RNA, Messenger) RN - 4TI98Z838E (Estradiol) SB - IM MH - Adipose Tissue/physiology/physiopathology MH - Animals MH - Blood Glucose/physiology MH - Brain-Derived Neurotrophic Factor/*metabolism MH - *Diet, Fat-Restricted MH - *Diet, High-Fat MH - Eating/physiology MH - Estradiol/blood MH - Fasting/*physiology MH - Female MH - Gene Expression/physiology MH - Hyperphagia/physiopathology MH - Leptin/blood MH - Male MH - Obesity/physiopathology MH - RNA, Messenger/metabolism MH - Rats, Long-Evans MH - *Sex Characteristics MH - Ventromedial Hypothalamic Nucleus/*physiology/physiopathology MH - Weight Gain/physiology PMC - PMC4041709 MID - NIHMS584362 OTO - NOTNLM OT - Dietary intervention OT - Estradiol OT - Glucose OT - High-fat diet OT - Leptin EDAT- 2014/04/09 06:00 MHDA- 2014/12/19 06:00 PMCR- 2015/05/10 CRDT- 2014/04/09 06:00 PHST- 2013/12/16 00:00 [received] PHST- 2014/03/04 00:00 [revised] PHST- 2014/03/26 00:00 [accepted] PHST- 2014/04/09 06:00 [entrez] PHST- 2014/04/09 06:00 [pubmed] PHST- 2014/12/19 06:00 [medline] PHST- 2015/05/10 00:00 [pmc-release] AID - S0031-9384(14)00174-7 [pii] AID - 10.1016/j.physbeh.2014.03.028 [doi] PST - ppublish SO - Physiol Behav. 2014 May 10;130:99-107. doi: 10.1016/j.physbeh.2014.03.028. Epub 2014 Apr 4.