PMID- 24710795
OWN - NLM
STAT- MEDLINE
DCOM- 20150219
LR  - 20211021
IS  - 1573-9686 (Electronic)
IS  - 0090-6964 (Print)
IS  - 0090-6964 (Linking)
VI  - 42
IP  - 6
DP  - 2014 Jun
TI  - Electrical and neurotrophin enhancement of neurite outgrowth within a 3D collagen 
      scaffold.
PG  - 1282-91
LID - 10.1007/s10439-014-1001-0 [doi]
AB  - Electrical and chemical stimulation have been studied as potent mechanisms of 
      enhancing nerve regeneration and wound healing. However, it remains unclear how 
      electrical stimuli affect nerve growth, particularly in the presence of 
      neurotrophic factors. The objective of this study was to explore (1) the effect 
      of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) 
      supplementation to support neurite outgrowth in a 3D scaffold, and (2) the effect 
      of brief, low voltage, electrical stimulation (ES) on neurite outgrowth prior to 
      neurotrophin supplementation. Dissociated E11 chick dorsal root ganglia (DRG) 
      were seeded within a 1.5 mg/mL type-I collagen scaffold. For neurotrophin 
      treatments, scaffolds were incubated for 24 h in culture media containing NGF 
      (10 ng/mL) or BDNF (200 ng/mL), or both. For ES groups, scaffolds containing 
      neurons were stimulated for 10 min at 8-10 V/m DC, then incubated for 24 h with 
      neurotrophin. Fixed and labeled neurons were imaged to measure neurite growth and 
      directionality. BDNF supplementation was not as effective as NGF at supporting 
      DRG neurite outgrowth. ES prior to NGF supplementation improved DRG neurite 
      outgrowth compared to NGF alone. This combination of brief ES with NGF treatment 
      was the most effective treatment compared to NGF or BDNF alone. Brief ES had no 
      impact on neurite directionality in the 3D scaffolds. These results demonstrate 
      that ES improves neurite outgrowth in the presence of neurotrophins, and could 
      provide a potential therapeutic approach to improve nerve regeneration when 
      coupled with neurotrophin treatment.
FAU - Adams, Robert D
AU  - Adams RD
AD  - Department of Pharmacological and Physiological Science, Saint Louis University, 
      1402 S. Grand Blvd, St. Louis, MO, 63104, USA.
FAU - Rendell, Sara R
AU  - Rendell SR
FAU - Counts, Lauren R
AU  - Counts LR
FAU - Papke, Jason B
AU  - Papke JB
FAU - Willits, Rebecca K
AU  - Willits RK
FAU - Harkins, Amy B
AU  - Harkins AB
LA  - eng
GR  - T32 GM008306/GM/NIGMS NIH HHS/United States
GR  - 5T 32 GM008306-24/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20140408
PL  - United States
TA  - Ann Biomed Eng
JT  - Annals of biomedical engineering
JID - 0361512
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 9007-34-5 (Collagen)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Collagen/*chemistry
MH  - Electric Stimulation
MH  - Ganglia, Spinal/cytology/*metabolism
MH  - Nerve Growth Factor/metabolism
MH  - Nerve Growth Factors/metabolism
MH  - Neurites/*metabolism
MH  - Tissue Scaffolds/*chemistry
PMC - PMC4735875
MID - NIHMS753733
EDAT- 2014/04/09 06:00
MHDA- 2015/02/20 06:00
PMCR- 2016/02/02
CRDT- 2014/04/09 06:00
PHST- 2013/11/27 00:00 [received]
PHST- 2014/03/28 00:00 [accepted]
PHST- 2014/04/09 06:00 [entrez]
PHST- 2014/04/09 06:00 [pubmed]
PHST- 2015/02/20 06:00 [medline]
PHST- 2016/02/02 00:00 [pmc-release]
AID - 10.1007/s10439-014-1001-0 [doi]
PST - ppublish
SO  - Ann Biomed Eng. 2014 Jun;42(6):1282-91. doi: 10.1007/s10439-014-1001-0. Epub 2014 
      Apr 8.