PMID- 24710824
OWN - NLM
STAT- MEDLINE
DCOM- 20150707
LR  - 20211021
IS  - 1532-2807 (Electronic)
IS  - 1219-4956 (Linking)
VI  - 20
IP  - 4
DP  - 2014 Oct
TI  - Frequency of genetic and epigenetic alterations of p14ARF and p16INK4A in head 
      and neck cancer in a Hungarian population.
PG  - 923-9
LID - 10.1007/s12253-014-9775-9 [doi]
AB  - Occurrence of genetic and epigenetic alterations affecting p14ARF and p16INK4A 
      were investigated in tumour samples of 37 oral (OSCC) and 28 laryngeal squamous 
      cell cancer (LSCC) patients, and compared to exfoliated buccal epithelial cells 
      of 68 healthy controls. Presence of deletions and mutations/polymorphisms 
      affecting exons were examined using sequencing. Methylation status of promoters 
      was assessed by methylation-specific PCR. Chi-square and Fisher's exact tests 
      were used to compare frequency of events. Exon deletions were found in four 
      controls, one OSCC and 22 LSCC patients; the latter significantly differed from 
      controls (p < 0.001). Only two mutations (T24610A and C24702A) were in p16 exon 1 
      of two OSCC patients. Polymorphisms G28575A (Ala140Thr), G31292C (C540G) and 
      G28608A were found in both patient groups. The p14 promoter was unmethylated in 
      86.7 % of OSCC and in 85.7 % of LSCC patients; for the p16 promoter these rates 
      were 69.0 % and 76.2 % for OSCC and LSCC patients, respectively. Combining the 
      two patient groups, unmethylated promoter was significantly less frequent in case 
      of both p14 and p16 (p = 0.043 and p = 0.001, respectively) compared to the 
      control group. In summary, exon deletion may be important in LSCC, while promoter 
      methylation was relatively frequent in both patient groups.
FAU - Kis, Andrea
AU  - Kis A
AD  - Department of Medical Microbiology, Faculty of Medicine, University of Debrecen, 
      H-4032, Debrecen, Nagyerdei krt. 98, Hungary.
FAU - Tatar, Timea Zsofia
AU  - Tatar TZ
FAU - Gall, Tamas
AU  - Gall T
FAU - Boda, Robert
AU  - Boda R
FAU - Tar, Ildiko
AU  - Tar I
FAU - Major, Tamas
AU  - Major T
FAU - Redl, Pal
AU  - Redl P
FAU - Gergely, Lajos
AU  - Gergely L
FAU - Szarka, Krisztina
AU  - Szarka K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140409
PL  - Switzerland
TA  - Pathol Oncol Res
JT  - Pathology oncology research : POR
JID - 9706087
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
RN  - 0 (Tumor Suppressor Protein p14ARF)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/epidemiology/*genetics/mortality
MH  - Case-Control Studies
MH  - Cyclin-Dependent Kinase Inhibitor p16/*genetics
MH  - DNA Methylation
MH  - Epigenesis, Genetic/*genetics
MH  - Female
MH  - Follow-Up Studies
MH  - *Gene Deletion
MH  - Gene Silencing
MH  - Humans
MH  - Hungary/epidemiology
MH  - Laryngeal Neoplasms/epidemiology/*genetics/mortality
MH  - Male
MH  - Middle Aged
MH  - Mouth Neoplasms/epidemiology/*genetics/mortality
MH  - Mutation/genetics
MH  - Prognosis
MH  - Promoter Regions, Genetic/genetics
MH  - Survival Rate
MH  - Tumor Suppressor Protein p14ARF/*genetics
EDAT- 2014/04/09 06:00
MHDA- 2015/07/08 06:00
CRDT- 2014/04/09 06:00
PHST- 2013/10/21 00:00 [received]
PHST- 2014/03/27 00:00 [accepted]
PHST- 2014/04/09 06:00 [entrez]
PHST- 2014/04/09 06:00 [pubmed]
PHST- 2015/07/08 06:00 [medline]
AID - 10.1007/s12253-014-9775-9 [doi]
PST - ppublish
SO  - Pathol Oncol Res. 2014 Oct;20(4):923-9. doi: 10.1007/s12253-014-9775-9. Epub 2014 
      Apr 9.