PMID- 24710867 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20140409 LR - 20220317 IS - 0040-5752 (Print) IS - 0040-5752 (Linking) VI - 96 IP - 3-4 DP - 1998 Mar TI - Quantitative karyotyping of three diploid Brassica species by imaging methods and localization of 45s rDNA loci on the identified chromosomes. PG - 325-30 LID - 10.1007/s001220050744 [doi] AB - Chromosomes of the three diploid Brassica species, B. rapa (AA), B. nigra (BB) and B. oleracea (CC), were identified based on their morphological characteristics, especially on the condensation pattern appearing at the somatic pro-metaphase stage. The morphological features of the pro-metaphase chromosomes of the three Brassica spp. were quantified by imaging methods using chromosome image analyzing system II (CHIAS 2). As a result, quantitative chromosome maps or idiograms of the three diploid Brassica spp. were developed. The fluorescence in situ hybridization (FISH) method revealed the location of 45s rDNA (the 26s-5.8s-18s ribosomal RNA gene cluster) on the chromosomes involved. The number of 45s rDNA loci in the B. rapa, B. nigra and B. oleracea are five, three and two, respectively. The loci detected were systematically mapped on the idiograms of the three Brassica spp. FAU - Fukui, K AU - Fukui K AD - Hokuriku National Agricultural Experiment Station, Joetsu 943-01, Japan Fax: 81-255-26-3238 E-mail: kfukui@inada.affrc.go.jp, JP. FAU - Nakayama, S AU - Nakayama S FAU - Ohmido, N AU - Ohmido N FAU - Yoshiaki, H AU - Yoshiaki H FAU - Yamabe, M AU - Yamabe M LA - eng PT - Journal Article PL - Germany TA - Theor Appl Genet JT - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JID - 0145600 EDAT- 1998/03/01 00:00 MHDA- 1998/03/01 00:01 CRDT- 2014/04/09 06:00 PHST- 2014/04/09 06:00 [entrez] PHST- 1998/03/01 00:00 [pubmed] PHST- 1998/03/01 00:01 [medline] AID - 10.1007/s001220050744 [doi] PST - ppublish SO - Theor Appl Genet. 1998 Mar;96(3-4):325-30. doi: 10.1007/s001220050744.