PMID- 24712278
OWN - NLM
STAT- MEDLINE
DCOM- 20140430
LR  - 20200103
IS  - 0204-8043 (Print)
IS  - 0204-8043 (Linking)
VI  - 55
IP  - 3-4
DP  - 2013 Jul-Dec
TI  - Levels of certain endothelial biomarkers during the acute phase and convalescence 
      in patients with different severity of Mediterranean spotted fever.
PG  - 17-25
AB  - INTRODUCTION: Mediterranean spotted fever (MSF) is a tick-borne disease caused by 
      Rickettsia conorii subspp. conorii. It is transmitted by the bite of the tick 
      Rhipicephalus sanguineus. Modified by the rickettsial invasion, the 
      micro-vascular endothelium acquires an activated inflammatory phenotype and 
      initiates secretion of cytokines and expression of cell adhesion molecules (CAMs) 
      and chemoattractans. AIM: This study aims at investigating the alterations in the 
      soluble cellular adhesion molecules (sCAMs) and chemokine MCP-1 levels in 
      patients with MSF of varying severity in the acute and convalescence stage in 
      order to assess their diagnostic and prognostic value. MATERIALS AND METHODS: The 
      soluble forms of cellular adhesion molecules (sCAMs)--sE-selectin and 
      sP-selectin, the intercellular (sICAM-1) and vascular (sVCAM-1) adhesion 
      molecules as well as the monocyte chemoattractant protein-1 (MCP-1) were studied 
      in the sera of 80 patients with MSF. The presence of MSF was confirmed 
      serologically by indirect fluorescence assay (IFA). In order to study disease 
      dynamics, serum samples from 80 patients were drawn on day 1 following the onset 
      of rash; in 60 patients (part of the surveyed 80) a second sample was taken in 
      the convalescence period--14 days post hospital discharge. The investigation was 
      focused on mild, moderate and severe forms of MSF. Enzyme linked immune-sorbent 
      assay was used for sCAMs determination (Quantikine IVD colorimetric RESULTS: 
      Overall, in the acute stage, patients presented with increased levels of 
      sE-selectin, sICAM-1, sVCAM-1 and MCP-1, whereas sP-selectin level was decreased. 
      The levels of sE-selectin, sICAM-1 and sVCAM-1 were significantly elevated in 
      mild, moderate and severe forms of the disease with sE-selectin level exhibiting 
      a plateau tendency and sICAM and sVCAM levels demonstrating an upward trend from 
      mild towards severe MSF forms. MCP-1 level was elevated only in severe MSF. In 
      all forms of MSF, in the convalescence period, sICAM-1, sVCAM-1 and MCP-1 
      concentration returned to reference levels whereas sE-selectin level persisted 
      elevated. In the convalescence stage, sP- selectin concentration also showed an 
      upward tendency, which in severe forms of MSF slightly exceeded the level in 
      controls. sP-selectin levels correlated directly with platelet count, whereas 
      sICAM-1 and sVCAM-1 levels showed a reverse correlation, sE-selectin, sICAM-1 and 
      MCP-1 levels directly correlated with aminotransferase activity (ALT and/or AST). 
      CONCLUSION: The soluble forms of CAMs reflect the endothelial inflammatory 
      potential. There is evidence that endothelium activation is more potent in severe 
      forms of MSF. Assessment of the endothelial response in the course of the disease 
      is an important predictor of the outcome, the choice of therapeutic approach and 
      disease prognosis.
FAU - Baltadzhiev, Ivan G
AU  - Baltadzhiev IG
AD  - Department of Infections Diseases, Medical Faculty
FAU - Murdjeva, Mariana A
AU  - Murdjeva MA
AD  - Department of Microbiology and Immunology, Faculty of Pharmacy, Medical 
      University Plovdiv, Bulgaria
LA  - eng
PT  - Journal Article
PL  - Bulgaria
TA  - Folia Med (Plovdiv)
JT  - Folia medica
JID - 2984761R
RN  - 0 (Biomarkers)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Chemokine CCL2)
RN  - 0 (E-Selectin)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Acute Disease
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers/blood
MH  - Boutonneuse Fever/*blood/immunology
MH  - Cell Adhesion Molecules/*blood
MH  - Chemokine CCL2/*blood
MH  - Child
MH  - Child, Preschool
MH  - E-Selectin/blood
MH  - Endothelium, Vascular/*physiology
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/blood
MH  - Middle Aged
MH  - Vascular Cell Adhesion Molecule-1/blood
EDAT- 2014/04/10 06:00
MHDA- 2014/05/03 06:00
CRDT- 2014/04/10 06:00
PHST- 2014/04/10 06:00 [entrez]
PHST- 2014/04/10 06:00 [pubmed]
PHST- 2014/05/03 06:00 [medline]
AID - 10.2478/folmed-2013-0023 [doi]
PST - ppublish
SO  - Folia Med (Plovdiv). 2013 Jul-Dec;55(3-4):17-25. doi: 10.2478/folmed-2013-0023.