PMID- 24712657
OWN - NLM
STAT- MEDLINE
DCOM- 20170714
LR  - 20211204
IS  - 1756-185X (Electronic)
IS  - 1756-1841 (Linking)
VI  - 19
IP  - 5
DP  - 2016 May
TI  - Chlamydia trachomatis elementary bodies in synovial fluid of patients with 
      reactive arthritis and undifferentiated spondyloarthropathy in India.
PG  - 506-11
LID - 10.1111/1756-185X.12364 [doi]
AB  - OBJECTIVES: Reportedly, there is little information on the magnitude of 
      genitourinary-induced reactive arthritis (gReA) from India. Genital infection 
      with Chlamydia trachomatis is a major health problem in India because of its high 
      prevalence; therefore, this study was conducted with the aim to screen 
      ReA/undifferentiated spondyloarthropathy (uSpA) patients (n = 20) attending a 
      major city hospital in New Delhi, for investigating the presence of 
      intra-articular chlamydial antigen in knee joints. Patients with rheumatoid 
      arthritis (RA) and osteoarthritis (OA) served as controls (n = 20). METHODS: 
      Synovial fluid samples were screened for chlamydial elementary bodies (EBs) using 
      a commercial kit (MicroTrak C. trachomatis Direct Specimen Test; Trinity Biotech, 
      USA) for performing direct fluorescence assay (DFA). RESULTS: Chlamydia 
      trachomatis EBs were detected in the synovial fluid cell deposits of six patients 
      in Group I, namely, 33.3% (4/12) ReA and 25% (2/8) uSpA. All C. trachomatis 
      positive patients exhibited an oligoarticular clinical picture with knee joint 
      involvement. In the synovial fluid cell deposits of control patients, namely, 
      RA/OA, no chlamydial EBs could be detected. CONCLUSIONS: This is the first study 
      reporting the presence of C. trachomatis EBs in the synovial fluid of 
      spondyloarthropathy patients, namely, ReA/uSpA from our country and it can be 
      concluded that the prevalence of C. trachomatis-induced ReA is underestimated. 
      Although our study had limitations in terms of sample size and lower sensitivity 
      of DFA, yet this test can be used as an initial diagnostic tool for screening and 
      patients with positive results may undergo specific tests for validation.
CI  - (c) 2014 Asia Pacific League of Associations for Rheumatology and Wiley Publishing 
      Asia Pty Ltd.
FAU - Kumar, Praveen
AU  - Kumar P
AD  - Microbiology Laboratory, National Institute of Pathology (ICMR), New Delhi, 
      India.
FAU - Khanna, Geetika
AU  - Khanna G
AD  - Central Institute of Orthopedics (CIO), New Delhi, India.
AD  - Vardhaman Mahavir Medical College (VMMC) and Safdarjung Hospital, New Delhi, 
      India.
FAU - Batra, Sumit
AU  - Batra S
AD  - Central Institute of Orthopedics (CIO), New Delhi, India.
FAU - Sharma, Vinod K
AU  - Sharma VK
AD  - Central Institute of Orthopedics (CIO), New Delhi, India.
AD  - Vardhaman Mahavir Medical College (VMMC) and Safdarjung Hospital, New Delhi, 
      India.
FAU - Rastogi, Sangita
AU  - Rastogi S
AD  - Microbiology Laboratory, National Institute of Pathology (ICMR), New Delhi, 
      India.
LA  - eng
PT  - Journal Article
DEP - 20140409
PL  - England
TA  - Int J Rheum Dis
JT  - International journal of rheumatic diseases
JID - 101474930
RN  - 0 (Antigens, Bacterial)
RN  - 0 (PHB2 protein, human)
RN  - 0 (Prohibitins)
SB  - IM
MH  - Adult
MH  - Antigens, Bacterial/immunology/*isolation & purification
MH  - Chlamydia Infections/diagnosis/immunology/*microbiology
MH  - Chlamydia trachomatis/immunology/*isolation & purification
MH  - Female
MH  - Humans
MH  - India
MH  - Knee Joint/immunology/*microbiology
MH  - Male
MH  - Prohibitins
MH  - Spondylarthropathies/diagnosis/immunology/*microbiology
MH  - Synovial Fluid/immunology/*microbiology
MH  - Young Adult
OTO - NOTNLM
OT  - Chlamydia trachomatis
OT  - elementary body
OT  - reactive arthritis
OT  - synovial fluid
OT  - undifferentiated spondyloarthropathy
EDAT- 2014/04/10 06:00
MHDA- 2017/07/15 06:00
CRDT- 2014/04/10 06:00
PHST- 2014/04/10 06:00 [entrez]
PHST- 2014/04/10 06:00 [pubmed]
PHST- 2017/07/15 06:00 [medline]
AID - 10.1111/1756-185X.12364 [doi]
PST - ppublish
SO  - Int J Rheum Dis. 2016 May;19(5):506-11. doi: 10.1111/1756-185X.12364. Epub 2014 
      Apr 9.