PMID- 24712758
OWN - NLM
STAT- MEDLINE
DCOM- 20150706
LR  - 20211021
IS  - 1525-1594 (Electronic)
IS  - 0160-564X (Print)
IS  - 0160-564X (Linking)
VI  - 38
IP  - 11
DP  - 2014 Nov
TI  - Activation of basophils is a new and sensitive marker of biocompatibility in 
      hemodialysis.
PG  - 945-53
LID - 10.1111/aor.12297 [doi]
AB  - The hemodialysis procedure involves contact between peripheral blood and the 
      surface of dialyzer membranes, which may lead to alterations in the pathways of 
      innate and adaptive immunity. We aimed to study the effect of blood-membrane 
      interaction on human peripheral basophils and neutrophils in hemodialysis with 
      high- and low-permeability polysulfone dialyzers. The surface expression of 
      CD203c (basophil selection marker) and CD63 (activation marker) after activation 
      by the bacterial peptide formyl-methionyl-leucyl-phenylalanine (fMLP) or anti-Fcepsilon 
      receptor I (FcepsilonRI) antibody and the absolute number of basophils was investigated 
      before and after hemodialysis with each of the dialyzers. Moreover, the 
      expression on neutrophils of CD11b, the CD11b active epitope, and CD88 was 
      analyzed in the same groups of individuals. The expression of CD63 in basophils 
      following activation by fMLP was significantly higher in the patient group 
      compared with that in healthy controls, but no differences were observed after 
      activation by anti-FcepsilonRI. During the hemodialysis procedure, the low-flux 
      membrane induced up-regulation of CD63 expression on basophils, while passage 
      through the high-flux membrane did not significantly alter the responsiveness. In 
      addition, the absolute number of basophils was unchanged after hemodialysis with 
      either of the dialyzers and compared with healthy controls. We found no 
      significant differences in the expression of the neutrophil activation markers 
      (CD11b, the active epitope of CD11b, and CD88) comparing the two different 
      dialyzers before and after dialysis and healthy controls. Together, these 
      findings suggest that alterations in basophil activity may be a useful marker of 
      membrane bioincompatibility in hemodialysis.
CI  - (c) 2014 The Authors. Artificial Organs published by The International Center for 
      Artificial Organs and Transplantation and Wiley Periodicals, Inc.
FAU - Aljadi, Zenib
AU  - Aljadi Z
AD  - Unit of Clinical Immunology and Allergy, Department of Medicine, Karolinska 
      University Hospital Solna, Karolinska Institute, Stockholm, Sweden; Division of 
      Proteomics and Nanobiotechnology, School of Biotechnology, Royal Institute of 
      Technology, Stockholm, Sweden.
FAU - Mansouri, Ladan
AU  - Mansouri L
FAU - Nopp, Anna
AU  - Nopp A
FAU - Paulsson, Josefin M
AU  - Paulsson JM
FAU - Winqvist, Ola
AU  - Winqvist O
FAU - Russom, Aman
AU  - Russom A
FAU - Stahl, Marten
AU  - Stahl M
FAU - Hylander, Britta
AU  - Hylander B
FAU - Jacobson, Stefan H
AU  - Jacobson SH
FAU - Lundahl, Joachim
AU  - Lundahl J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140409
PL  - United States
TA  - Artif Organs
JT  - Artificial organs
JID - 7802778
RN  - 0 (Biocompatible Materials)
RN  - 0 (Biomarkers)
RN  - 0 (C5AR1 protein, human)
RN  - 0 (CD11b Antigen)
RN  - 0 (CD63 protein, human)
RN  - 0 (ENPP3 protein, human)
RN  - 0 (ITGAM protein, human)
RN  - 0 (Membranes, Artificial)
RN  - 0 (Polymers)
RN  - 0 (Receptor, Anaphylatoxin C5a)
RN  - 0 (Sulfones)
RN  - 0 (Tetraspanin 30)
RN  - 25135-51-7 (polysulfone P 1700)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - EC 3.1.4.- (Phosphoric Diester Hydrolases)
RN  - EC 3.6.1.- (Pyrophosphatases)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Basophils/*metabolism
MH  - Biocompatible Materials
MH  - Biomarkers/*blood
MH  - CD11b Antigen/blood
MH  - Comorbidity
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Kidney Failure, Chronic/*therapy
MH  - Male
MH  - *Membranes, Artificial
MH  - Middle Aged
MH  - N-Formylmethionine Leucyl-Phenylalanine
MH  - Neutrophils/physiology
MH  - Phosphoric Diester Hydrolases/blood
MH  - Polymers
MH  - Pyrophosphatases/blood
MH  - Receptor, Anaphylatoxin C5a/blood
MH  - Renal Dialysis/*methods
MH  - Sulfones
MH  - Tetraspanin 30/blood
PMC - PMC4257079
OTO - NOTNLM
OT  - Basophil activation
OT  - Biocompatibility
OT  - Chronic kidney disease
OT  - Hemodialysis
OT  - Neutrophil activation
EDAT- 2014/04/10 06:00
MHDA- 2015/07/07 06:00
CRDT- 2014/04/10 06:00
PHST- 2014/04/10 06:00 [entrez]
PHST- 2014/04/10 06:00 [pubmed]
PHST- 2015/07/07 06:00 [medline]
AID - 10.1111/aor.12297 [doi]
PST - ppublish
SO  - Artif Organs. 2014 Nov;38(11):945-53. doi: 10.1111/aor.12297. Epub 2014 Apr 9.