PMID- 2471425
OWN - NLM
STAT- MEDLINE
DCOM- 19890703
LR  - 20151119
IS  - 0003-276X (Print)
IS  - 0003-276X (Linking)
VI  - 224
IP  - 1
DP  - 1989 May
TI  - Acridine orange-RNA fluorescence of maturing neurons in the perinatal rat brain.
PG  - 88-93
AB  - Cytoplasmic RNA was demonstrated in neurons of the developing rat brain using 
      acridine orange (AO) as a histochemical marker. Fetuses of 18 days and postnatal 
      rats of 1, 7, 14, and 21 days as well as adults several months old were studied. 
      Neuroblasts of the germinal matrix exhibited minimal or no orange-red AO-RNA 
      fluorescence, but immature nerve cells in migration within the cerebral 
      hemispheres of the rat showed a weak but definite orange colour. This finding 
      contrasts with the absence of AO-RNA fluorescence in migrating human neuroblasts. 
      Neurons of the neocortical plate showed uniformly strong fluorescence. In the 
      hippocampus, the most pronounced increase in AO-demonstrated RNA was in pyramidal 
      and granule cells during the first postnatal week. The cerebellum showed a 
      paradoxically stronger fluorescence of granule cells in the 18-day fetus than at 
      birth, and almost no AO-RNA fluorescence of granule cells at 21 days of age or in 
      adults. Motor neurons showed the strongest fluorescence of all neurons. It is 
      likely that the increase in cytoplasmic RNA in neurons corresponds to the onset 
      of neurotransmitter biosynthesis, but transitory fetal neuropeptides may explain 
      stronger fluorescence of some neurons in young individuals. The reliable and 
      simple AO method provides a supplementary means of studying one aspect of 
      neuronal maturation.
FAU - Topaloglu, H
AU  - Topaloglu H
AD  - Hacettepe University School of Medicine, Hacettepe Children's Hospital, Ankara, 
      Turkey.
FAU - Sarnat, H B
AU  - Sarnat HB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anat Rec
JT  - The Anatomical record
JID - 0370540
RN  - 63231-63-0 (RNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Animals, Newborn
MH  - Brain/cytology/embryology/*metabolism
MH  - Cell Survival
MH  - Fluorescence
MH  - Neurons/*metabolism
MH  - RNA/*metabolism
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Tissue Distribution
EDAT- 1989/05/01 00:00
MHDA- 1989/05/01 00:01
CRDT- 1989/05/01 00:00
PHST- 1989/05/01 00:00 [pubmed]
PHST- 1989/05/01 00:01 [medline]
PHST- 1989/05/01 00:00 [entrez]
AID - 10.1002/ar.1092240111 [doi]
PST - ppublish
SO  - Anat Rec. 1989 May;224(1):88-93. doi: 10.1002/ar.1092240111.