PMID- 24714921
OWN - NLM
STAT- MEDLINE
DCOM- 20141218
LR  - 20211203
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 33
IP  - 6
DP  - 2014 Jun
TI  - Ubiquitin-fold modifier 1 inhibits apoptosis by suppressing the endoplasmic 
      reticulum stress response in Raw264.7 cells.
PG  - 1539-46
LID - 10.3892/ijmm.2014.1728 [doi]
AB  - Ubiquitin-fold modifier 1 (Ufm1) is a new member of the ubiquitin-like protein 
      family, and its biological function remains largely unknown, particularly in 
      macrophages. In this study, we demonstrate that Ufm1 expression is increased in 
      diabetic mouse resident peritoneal macrophages (RPMs) and in the mouse macrophage 
      cell line, Raw264.7, subjected to endoplasmic reticulum (ER) stress. Following 
      treatment of the cells with the ER stress inducers, thapsigargin (TG) or 
      tunicamycin (TM), the lentiviral short hairpin RNA (shRNA)-mediated knockdown of 
      Ufm1 increased the apoptosis of Raw264.7 cells. Furthermore, these cells had 
      higher expression levels of immunoglobulin heavy chain-binding protein (BiP) and 
      C/EBP homologous protein (CHOP), which are markers of the ER stress response. The 
      overexpression of Ufm1 induced by lentiviral infection in the Raw264.7 cells 
      treated with the ER stress inducers, TG or TM, resulted in the opposite effect. 
      Taken together, our results suggest that Ufm1 is upregulated in diabetic mouse 
      RPMs and in Raw264.7 cells in response to ER stress and that Ufm1 protects 
      macrophages against apoptosis. Thus, Ufm1 may be a novel gene that protects 
      against ER stress-induced apoptosis in macrophages.
FAU - Hu, Xiaolei
AU  - Hu X
AD  - Department of Endocrinology, Shanghai 3rd People's Hospital, School of Medicine, 
      Shanghai Jiao Tong University, Shanghai 201999, P.R. China.
FAU - Pang, Qi
AU  - Pang Q
AD  - Department of Endocrinology, Shanghai 3rd People's Hospital, School of Medicine, 
      Shanghai Jiao Tong University, Shanghai 201999, P.R. China.
FAU - Shen, Qiongna
AU  - Shen Q
AD  - Department of Endocrinology, Shanghai 3rd People's Hospital, School of Medicine, 
      Shanghai Jiao Tong University, Shanghai 201999, P.R. China.
FAU - Liu, Huifang
AU  - Liu H
AD  - Department of Endocrinology, Shanghai 3rd People's Hospital, School of Medicine, 
      Shanghai Jiao Tong University, Shanghai 201999, P.R. China.
FAU - He, Jiangping
AU  - He J
AD  - Department of Endocrinology, Shanghai 3rd People's Hospital, School of Medicine, 
      Shanghai Jiao Tong University, Shanghai 201999, P.R. China.
FAU - Wang, Jing
AU  - Wang J
AD  - Department of Endocrinology, Shanghai 3rd People's Hospital, School of Medicine, 
      Shanghai Jiao Tong University, Shanghai 201999, P.R. China.
FAU - Xiong, Jie
AU  - Xiong J
AD  - Department of Endocrinology, Shanghai 3rd People's Hospital, School of Medicine, 
      Shanghai Jiao Tong University, Shanghai 201999, P.R. China.
FAU - Zhang, Huijie
AU  - Zhang H
AD  - Department of Endocrinology, Shanghai 3rd People's Hospital, School of Medicine, 
      Shanghai Jiao Tong University, Shanghai 201999, P.R. China.
FAU - Chen, Fengling
AU  - Chen F
AD  - Department of Endocrinology, Shanghai 3rd People's Hospital, School of Medicine, 
      Shanghai Jiao Tong University, Shanghai 201999, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140407
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Endoplasmic Reticulum Chaperone BiP)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Ufm1 protein, mouse)
RN  - 147336-12-7 (Transcription Factor CHOP)
RN  - 67526-95-8 (Thapsigargin)
SB  - IM
MH  - Animals
MH  - Apoptosis/genetics/physiology
MH  - Cell Line
MH  - Endoplasmic Reticulum Chaperone BiP
MH  - Endoplasmic Reticulum Stress/genetics/*physiology
MH  - Heat-Shock Proteins/genetics/metabolism
MH  - Macrophages/*cytology/metabolism
MH  - Proteins/genetics/*metabolism
MH  - RNA, Small Interfering/genetics/physiology
MH  - Thapsigargin/metabolism
MH  - Transcription Factor CHOP/genetics/metabolism
EDAT- 2014/04/10 06:00
MHDA- 2014/12/19 06:00
CRDT- 2014/04/10 06:00
PHST- 2013/12/12 00:00 [received]
PHST- 2014/03/21 00:00 [accepted]
PHST- 2014/04/10 06:00 [entrez]
PHST- 2014/04/10 06:00 [pubmed]
PHST- 2014/12/19 06:00 [medline]
AID - 10.3892/ijmm.2014.1728 [doi]
PST - ppublish
SO  - Int J Mol Med. 2014 Jun;33(6):1539-46. doi: 10.3892/ijmm.2014.1728. Epub 2014 Apr 
      7.