PMID- 24717033
OWN - NLM
STAT- MEDLINE
DCOM- 20140529
LR  - 20191112
IS  - 0001-723X (Print)
IS  - 0001-723X (Linking)
VI  - 58
IP  - 1
DP  - 2014
TI  - Development of a one-step immunocapture real-time RT-PCR assay for the detection 
      of barley stripe mosaic virus strains in barley seedlings.
PG  - 81-5
AB  - A one-step immunocapture real-time RT-PCR (IC-real-time RT-PCR) was developed for 
      efficient detection of barley stripe mosaic virus (BSMV) in barley seedlings. The 
      novel detection system was designed using a primer set targeting the conserved 
      region in the triple gene block 2 (TGB2) to expand its capacity to detect all 
      BSMV strains. This assay was evaluated for its efficiency in detecting BSMV in 
      barley seedlings. Using the immunocapture sample preparation, real-time RT-PCR 
      was able to detect BSMV in samples, which were indicated as negative by ELISA. 
      The sensitivity of detection in the real-time RT-PCR was as low as 50 fg/microl of 
      total viral RNA under optimal reaction conditions. This level of sensitivity 
      indicated that the one-step IC-real-time RT-PCR developed in the present study 
      could be used for routine plant and seed health assays.
FAU - Zarzynska, A
AU  - Zarzynska A
FAU - Jezewska, M
AU  - Jezewska M
FAU - Trzmiel, K
AU  - Trzmiel K
FAU - Hasiow-Jaroszewska, B
AU  - Hasiow-Jaroszewska B
LA  - eng
PT  - Journal Article
PL  - Slovakia
TA  - Acta Virol
JT  - Acta virologica
JID - 0370401
SB  - IM
MH  - Hordeum/*virology
MH  - Mosaic Viruses/classification/*isolation & purification
MH  - Real-Time Polymerase Chain Reaction/*methods
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods
MH  - Seedlings/*virology
OTO - NOTNLM
OT  - barley stripe mosaic virus; barley; IC-real-time RT-PCR; diagnostics.
EDAT- 2014/04/11 06:00
MHDA- 2014/05/30 06:00
CRDT- 2014/04/11 06:00
PHST- 2014/04/11 06:00 [entrez]
PHST- 2014/04/11 06:00 [pubmed]
PHST- 2014/05/30 06:00 [medline]
AID - 10.4149/av_2014_01_81 [doi]
PST - ppublish
SO  - Acta Virol. 2014;58(1):81-5. doi: 10.4149/av_2014_01_81.