PMID- 24719862
OWN - NLM
STAT- MEDLINE
DCOM- 20141230
LR  - 20220408
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2014
DP  - 2014
TI  - Comparison of host immune responses to homologous and heterologous type II 
      porcine reproductive and respiratory syndrome virus (PRRSV) challenge in 
      vaccinated and unvaccinated pigs.
PG  - 416727
LID - 10.1155/2014/416727 [doi]
LID - 416727
AB  - Porcine reproductive and respiratory syndrome (PRRS) is a high-consequence animal 
      disease with current vaccines providing limited protection from infection due to 
      the high degree of genetic variation of field PRRS virus. Therefore, 
      understanding host immune responses elicited by different PRRSV strains will 
      facilitate the development of more effective vaccines. Using IngelVac modified 
      live PRRSV vaccine (MLV), its parental strain VR-2332, and the heterologous 
      KS-06-72109 strain (a Kansas isolate of PRRSV), we compared immune responses 
      induced by vaccination and/or PRRSV infection. Our results showed that MLV can 
      provide complete protection from homologous virus (VR-2332) and partial 
      protection from heterologous (KS-06) challenge. The protection was associated 
      with the levels of PRRSV neutralizing antibodies at the time of challenge, with 
      vaccinated pigs having higher titers to VR-2332 compared to KS-06 strain. 
      Challenge strain did not alter the cytokine expression profiles in the serum of 
      vaccinated pigs or subpopulations of T cells. However, higher frequencies of 
      IFN-gamma-secreting PBMCs were generated from pigs challenged with heterologous PRRSV 
      in a recall response when PBMCs were re-stimulated with PRRSV. Thus, this study 
      indicates that serum neutralizing antibody titers are associated with PRRSV 
      vaccination-induced protection against homologous and heterologous challenge.
FAU - Li, X
AU  - Li X
AD  - Department of Anatomy and Physiology, 228 Coles Hall, College of Veterinary 
      Medicine, Kansas State University, 1600 Denison Ave, Manhattan, KS 66506, USA.
FAU - Galliher-Beckley, A
AU  - Galliher-Beckley A
AD  - Department of Anatomy and Physiology, 228 Coles Hall, College of Veterinary 
      Medicine, Kansas State University, 1600 Denison Ave, Manhattan, KS 66506, USA.
FAU - Pappan, L
AU  - Pappan L
AD  - Department of Anatomy and Physiology, 228 Coles Hall, College of Veterinary 
      Medicine, Kansas State University, 1600 Denison Ave, Manhattan, KS 66506, USA ; 
      Intrexon Corporation, 108 TW Alexander Drive, Durham, NC 27709, USA.
FAU - Trible, B
AU  - Trible B
AD  - Department of Diagnostic Medicine and Pathobiology, College of Veterinary 
      Medicine, Kansas State University, 1600 Denison Ave, Manhattan, KS 66506, USA.
FAU - Kerrigan, M
AU  - Kerrigan M
AD  - Department of Diagnostic Medicine and Pathobiology, College of Veterinary 
      Medicine, Kansas State University, 1600 Denison Ave, Manhattan, KS 66506, USA.
FAU - Beck, A
AU  - Beck A
AD  - Department of Diagnostic Medicine and Pathobiology, College of Veterinary 
      Medicine, Kansas State University, 1600 Denison Ave, Manhattan, KS 66506, USA ; 
      The Scripps Research Institute, 130 Scripps Way No. 3B3, Jupiter, FL 33487, USA.
FAU - Hesse, R
AU  - Hesse R
AD  - Department of Diagnostic Medicine and Pathobiology, College of Veterinary 
      Medicine, Kansas State University, 1600 Denison Ave, Manhattan, KS 66506, USA.
FAU - Blecha, F
AU  - Blecha F
AD  - Department of Anatomy and Physiology, 228 Coles Hall, College of Veterinary 
      Medicine, Kansas State University, 1600 Denison Ave, Manhattan, KS 66506, USA.
FAU - Nietfeld, J C
AU  - Nietfeld JC
AD  - Department of Diagnostic Medicine and Pathobiology, College of Veterinary 
      Medicine, Kansas State University, 1600 Denison Ave, Manhattan, KS 66506, USA.
FAU - Rowland, R R
AU  - Rowland RR
AD  - Department of Diagnostic Medicine and Pathobiology, College of Veterinary 
      Medicine, Kansas State University, 1600 Denison Ave, Manhattan, KS 66506, USA.
FAU - Shi, J
AU  - Shi J
AD  - Department of Anatomy and Physiology, 228 Coles Hall, College of Veterinary 
      Medicine, Kansas State University, 1600 Denison Ave, Manhattan, KS 66506, USA.
LA  - eng
GR  - R21 AI085416/AI/NIAID NIH HHS/United States
GR  - NIH R21 AI085416/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140226
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Antibodies, Viral)
RN  - 0 (Vaccines, Attenuated)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/immunology
MH  - *Immunity, Innate
MH  - Interferon-gamma/immunology
MH  - Leukocytes, Mononuclear/immunology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/*immunology/pathogenicity
MH  - Swine
MH  - T-Lymphocytes/immunology
MH  - *Vaccination
MH  - Vaccines, Attenuated/administration & dosage/immunology
PMC - PMC3955659
EDAT- 2014/04/11 06:00
MHDA- 2014/12/31 06:00
PMCR- 2014/02/26
CRDT- 2014/04/11 06:00
PHST- 2013/11/18 00:00 [received]
PHST- 2014/01/05 00:00 [revised]
PHST- 2014/01/08 00:00 [accepted]
PHST- 2014/04/11 06:00 [entrez]
PHST- 2014/04/11 06:00 [pubmed]
PHST- 2014/12/31 06:00 [medline]
PHST- 2014/02/26 00:00 [pmc-release]
AID - 10.1155/2014/416727 [doi]
PST - ppublish
SO  - Biomed Res Int. 2014;2014:416727. doi: 10.1155/2014/416727. Epub 2014 Feb 26.