PMID- 24719865
OWN - NLM
STAT- MEDLINE
DCOM- 20141230
LR  - 20211021
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2014
DP  - 2014
TI  - Differential host cell gene expression and regulation of cell cycle progression 
      by nonstructural protein 11 of porcine reproductive and respiratory syndrome 
      virus.
PG  - 430508
LID - 10.1155/2014/430508 [doi]
LID - 430508
AB  - Nonstructural protein 11 (nsp11) of porcine reproductive and respiratory syndrome 
      virus (PRRSV) is a viral endoribonuclease with an unknown function. The 
      regulation of cellular gene expression by nsp11 was examined by RNA microarrays 
      using MARC-nsp11 cells constitutively expressing nsp11. In these cells, the 
      interferon-beta, interferon regulatory factor 3, and nuclear factor-kappa B activities 
      were suppressed compared to those of parental cells, suggesting that nsp11 might 
      serve as a viral interferon antagonist. Differential cellular transcriptome was 
      examined using Affymetrix exon chips representing 28,536 transcripts, and after 
      statistical analyses 66 cellular genes were shown to be upregulated and 104 genes 
      were downregulated by nsp11. These genes were grouped into 5 major signaling 
      pathways according to their functional relations: histone-related, cell cycle and 
      DNA replication, mitogen activated protein kinase signaling, complement, and 
      ubiquitin-proteasome pathways. Of these, the modulation of cell cycle by nsp11 
      was further investigated since many of the regulated genes fell in this 
      particular pathway. Flow cytometry showed that nsp11 caused the delay of cell 
      cycle progression at the S phase and the BrdU staining confirmed the cell cycle 
      arrest in nsp11-expressing cells. The study provides insights into the 
      understanding of specific cellular responses to nsp11 during PRRSV infection.
FAU - Sun, Yan
AU  - Sun Y
AD  - Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 
      South Lincoln Avenue, Urbana, IL 61802, USA ; Department of Pathobiology, 
      University of Pennsylvania, 380 South University Avenue, Philadelphia, PA 19104, 
      USA.
FAU - Li, Dong
AU  - Li D
AUID- ORCID: 0000-0003-3331-3268
AD  - Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 
      South Lincoln Avenue, Urbana, IL 61802, USA.
FAU - Giri, Sumanprava
AU  - Giri S
AUID- ORCID: 0000-0003-1218-3329
AD  - Department of Cell and Developmental Biology, University of Illinois at 
      Urbana-Champaign, Urbana, IL 61801, USA.
FAU - Prasanth, Supriya G
AU  - Prasanth SG
AD  - Department of Cell and Developmental Biology, University of Illinois at 
      Urbana-Champaign, Urbana, IL 61801, USA.
FAU - Yoo, Dongwan
AU  - Yoo D
AUID- ORCID: 0000-0002-6730-9729
AD  - Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 
      South Lincoln Avenue, Urbana, IL 61802, USA.
LA  - eng
GR  - R01 GM099669/GM/NIGMS NIH HHS/United States
GR  - NIH RO1GM099669/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20140226
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 77238-31-4 (Interferon-beta)
SB  - IM
MH  - Animals
MH  - Cell Cycle/genetics
MH  - Cell Cycle Proteins/*biosynthesis
MH  - Cell Line
MH  - Gene Expression Regulation, Viral
MH  - Interferon-beta/genetics
MH  - Porcine Reproductive and Respiratory Syndrome/*genetics/virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/pathogenicity
MH  - Swine
MH  - Viral Nonstructural Proteins/*biosynthesis
MH  - Virus Replication/genetics
PMC - PMC3955671
EDAT- 2014/04/11 06:00
MHDA- 2014/12/31 06:00
PMCR- 2014/02/26
CRDT- 2014/04/11 06:00
PHST- 2013/10/17 00:00 [received]
PHST- 2014/01/07 00:00 [accepted]
PHST- 2014/04/11 06:00 [entrez]
PHST- 2014/04/11 06:00 [pubmed]
PHST- 2014/12/31 06:00 [medline]
PHST- 2014/02/26 00:00 [pmc-release]
AID - 10.1155/2014/430508 [doi]
PST - ppublish
SO  - Biomed Res Int. 2014;2014:430508. doi: 10.1155/2014/430508. Epub 2014 Feb 26.