PMID- 24720634
OWN - NLM
STAT- MEDLINE
DCOM- 20151117
LR  - 20140611
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 6
IP  - 11
DP  - 2014 Jun 11
TI  - Silver nanoparticles and growth factors incorporated hydroxyapatite coatings on 
      metallic implant surfaces for enhancement of osteoinductivity and antibacterial 
      properties.
PG  - 8580-9
LID - 10.1021/am501428e [doi]
AB  - Research on incorporation of both growth factors and silver (Ag) into 
      hydroxyapatite (HA) coatings on metallic implant surfaces for enhancing 
      osteoinductivity and antibacterial properties is a challenging work. Generally, 
      Ag nanoparticles are easy to agglomerate and lead to a large increase in local Ag 
      concentration, which could potentially affect cell activity. On the other hand, 
      growth factors immobilization requires mild processing conditions so as to 
      maintain their activities. In this study, bone morphology protein-2 (BMP-2) and 
      Ag nanoparticle contained HA coatings were prepared on Ti surfaces by combining 
      electrochemical deposition (ED) of Ag and electrostatic immobilization of BMP-2. 
      During the ED process, chitosan (CS) was selected as the stabilizing agent to 
      chelate Ag ions and generate Ag nanoparticles that are uniformly distributed in 
      the coatings. CS also reduces Ag toxicity while retaining its antibacterial 
      activity. Afterwards, a BMP/heparin solution was absorbed on the CS/Ag/HA 
      coatings. Consequently, BMP-2 was immobilized on the coatings by the 
      electrostatic attraction between CS, heparin, and BMP-2. Sustained release of 
      BMP-2 and Ag ions from HA coatings was successfully demonstrated for a long 
      period. Results of antibacterial tests indicate that the CS/Ag/HA coatings have 
      high antibacterial properties against both Staphylococcus epidermidis and 
      Escherichia coli. Osteoblasts (OB) culture reveals that the CS/Ag/HA coatings 
      exhibit good biocompatibility. Bone marrow stromal cells (BMSCs) culture 
      indicates that the BMP/CS/Ag/HA coatings have good osteoinductivity and promote 
      the differentiation of BMSCs. Ti bars with BMP/CS/Ag/HA coatings were implanted 
      into the femur of rabbits to evaluate the osteoinductivity of the coatings. 
      Results indicate that BMP/CS/Ag/HA coatings favor bone formation in vivo. In 
      summary, this study presents a convenient and effective method for the 
      incorporation of growth factors and antibacterial agents into HA coatings. This 
      method can be utilized to modify a variety of metallic implant surfaces.
FAU - Xie, Chao-Ming
AU  - Xie CM
AD  - Key Lab of Advanced Technologies of Materials, Ministry of Education, School of 
      Materials Science and Engineering, Southwest Jiaotong University , Chengdu, 
      Sichuan 610031, China.
FAU - Lu, Xiong
AU  - Lu X
FAU - Wang, Ke-Feng
AU  - Wang KF
FAU - Meng, Fan-Zhi
AU  - Meng FZ
FAU - Jiang, Ou
AU  - Jiang O
FAU - Zhang, Hong-Ping
AU  - Zhang HP
FAU - Zhi, Wei
AU  - Zhi W
FAU - Fang, Li-Ming
AU  - Fang LM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140421
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 0 (Anti-Bacterial Agents)
RN  - 3M4G523W1G (Silver)
RN  - 91D9GV0Z28 (Durapatite)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Durapatite/*chemistry
MH  - Metal Nanoparticles/*chemistry
MH  - Microscopy, Electron, Scanning
MH  - Microscopy, Electron, Transmission
MH  - Osteoblasts/*drug effects
MH  - Rabbits
MH  - Silver/*chemistry
MH  - Surface Properties
EDAT- 2014/04/12 06:00
MHDA- 2015/11/18 06:00
CRDT- 2014/04/12 06:00
PHST- 2014/04/12 06:00 [entrez]
PHST- 2014/04/12 06:00 [pubmed]
PHST- 2015/11/18 06:00 [medline]
AID - 10.1021/am501428e [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2014 Jun 11;6(11):8580-9. doi: 10.1021/am501428e. Epub 
      2014 Apr 21.