PMID- 24722673
OWN - NLM
STAT- MEDLINE
DCOM- 20141230
LR  - 20240314
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 4
DP  - 2014
TI  - Wolbachia infect ovaries in the course of their maturation: last minute 
      passengers and priority travellers?
PG  - e94577
LID - 10.1371/journal.pone.0094577 [doi]
LID - e94577
AB  - Wolbachia are widespread endosymbiotic bacteria of arthropods and nematodes. 
      Studies on such models suggest that Wolbachia's remarkable aptitude to infect 
      offspring may rely on a re-infection of ovaries from somatic tissues instead of 
      direct cellular segregation between oogonia and oocytes. In the terrestrial 
      isopod Armadillidium vulgare, Wolbachia are vertically transmitted to the host 
      offspring, even though ovary cells are cyclically renewed. Using Fluorescence in 
      situ hybridization (FISH), we showed that the proportion of infected oocytes 
      increased in the course of ovary and oocyte maturation, starting with 31.5% of 
      infected oocytes only. At the end of ovary maturation, this proportion reached 
      87.6% for the most mature oocytes, which is close to the known transmission rate 
      to offspring. This enrichment can be explained by a secondary acquisition of the 
      bacteria by oocytes (Wolbachia can be seen as last minute passengers) and/or by a 
      preferential selection of oocytes infected with Wolbachia (as priority 
      travellers).
FAU - Genty, Lise-Marie
AU  - Genty LM
AD  - Equipe Ecologie Evolution Symbiose, Laboratoire Ecologie et Biologie des 
      Interactions, UMR CNRS 7267, Universite de Poitiers, Poitiers, France.
FAU - Bouchon, Didier
AU  - Bouchon D
AD  - Equipe Ecologie Evolution Symbiose, Laboratoire Ecologie et Biologie des 
      Interactions, UMR CNRS 7267, Universite de Poitiers, Poitiers, France.
FAU - Raimond, Maryline
AU  - Raimond M
AD  - Equipe Ecologie Evolution Symbiose, Laboratoire Ecologie et Biologie des 
      Interactions, UMR CNRS 7267, Universite de Poitiers, Poitiers, France.
FAU - Bertaux, Joanne
AU  - Bertaux J
AD  - Equipe Ecologie Evolution Symbiose, Laboratoire Ecologie et Biologie des 
      Interactions, UMR CNRS 7267, Universite de Poitiers, Poitiers, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140410
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Animals
MH  - Bacterial Load
MH  - Cell Size
MH  - Female
MH  - Host-Pathogen Interactions
MH  - In Situ Hybridization, Fluorescence
MH  - Isopoda/growth & development/*microbiology
MH  - Oocytes/growth & development/*microbiology
MH  - Ovary/growth & development/*microbiology
MH  - Wolbachia/*physiology
PMC - PMC3983217
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/04/12 06:00
MHDA- 2014/12/31 06:00
PMCR- 2014/04/10
CRDT- 2014/04/12 06:00
PHST- 2013/10/30 00:00 [received]
PHST- 2014/03/18 00:00 [accepted]
PHST- 2014/04/12 06:00 [entrez]
PHST- 2014/04/12 06:00 [pubmed]
PHST- 2014/12/31 06:00 [medline]
PHST- 2014/04/10 00:00 [pmc-release]
AID - PONE-D-13-44292 [pii]
AID - 10.1371/journal.pone.0094577 [doi]
PST - epublish
SO  - PLoS One. 2014 Apr 10;9(4):e94577. doi: 10.1371/journal.pone.0094577. eCollection 
      2014.