PMID- 24722810
OWN - NLM
STAT- MEDLINE
DCOM- 20150731
LR  - 20211021
IS  - 1573-4838 (Electronic)
IS  - 0957-4530 (Linking)
VI  - 25
IP  - 10
DP  - 2014 Oct
TI  - Conjugation of amino-bioactive glasses with 5-aminofluorescein as probe molecule 
      for the development of pH sensitive stimuli-responsive biomaterials.
PG  - 2243-53
LID - 10.1007/s10856-014-5206-4 [doi]
AB  - Bioceramics, such as silica-based glasses, are widely used in bone and teeth 
      restoration. Nowadays, the association between nanotechnology and pharmacology is 
      one of the most promising research fields in cancer therapy. The advanced 
      processing methods and new chemical strategies allow the incorporation of drugs 
      within them or on their functionalized surfaces. Bioceramics can act as local 
      drug delivery systems to treat bone and teeth diseases. The present paper reports 
      data related to the development of a pH-stimuli responsive bioactive glass. The 
      glass conjugation with 5-aminofluorescein (5-AF), through a pH-sensitive organic 
      spacer, allows to produce a pH-responsive bioactive biomaterial: when it is 
      exposed to specific pH changes, it can favour the release of 5-AF directly at the 
      target site. 5-AF has been chosen as a simple, low cost, non toxic model to 
      simulate doxorubicin, an anticancer drug. As doxorubicin, 5-AF contains an amino 
      group in its structure in order to form an amide bond with the carboxylic 
      functionalities of the glass. Raman spectroscopy and thermal analysis confirm the 
      glass conjugation of 5-AF by means of an amide bond; the amount of 5-AF loaded 
      was very high ( approximately  65 and 44 wt%). The release tests at two different pH (4.2 and 
      7.4) show that the amount of released 5-AF is higher at acid pH with respect to 
      physiological one. This preliminary datum evidenced that a pH-sensitive drug 
      delivery system has been developed. The low amount of 5-AF released (<1 wt% of 
      the total 5-AF) is due to the very low solubility of 5-AF in aqueous medium. This 
      disadvantage, may be overcome in a dynamic environment (physiological 
      conditions), where it is possible to obtain a drug release system ensuring an 
      effective therapeutic dose for long times and, at the same time, avoiding the 
      drug toxicity.
FAU - Aina, Valentina
AU  - Aina V
AD  - Department of Chemistry, Interdepartmental NIS (Nanostructured Interfaces and 
      Surfaces); INSTM (Italian National Consortium for Materials Science and 
      Technology), University of Torino, Via P. Giuria 7, 10125, Turin, Italy, 
      valentina.aina@unito.it.
FAU - Malavasi, Gianluca
AU  - Malavasi G
FAU - Magistris, Claudio
AU  - Magistris C
FAU - Cerrato, Giuseppina
AU  - Cerrato G
FAU - Martra, Gianmario
AU  - Martra G
FAU - Viscardi, Guido
AU  - Viscardi G
FAU - Menabue, Ledi
AU  - Menabue L
FAU - Lusvardi, Gigliola
AU  - Lusvardi G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140411
PL  - United States
TA  - J Mater Sci Mater Med
JT  - Journal of materials science. Materials in medicine
JID - 9013087
RN  - 0 (Biocompatible Materials)
RN  - 0 (Drug Carriers)
RN  - 0 (Fluoresceins)
RN  - 0 (Maleates)
RN  - 0 (Maleic Anhydrides)
RN  - 0 (Molecular Probes)
RN  - 0 (Propylamines)
RN  - 0 (Silanes)
RN  - 3326-34-9 (5-aminofluorescein)
RN  - L8S6UBW552 (amino-propyl-triethoxysilane)
RN  - MJS1DTX3X1 (maleamic acid)
SB  - IM
MH  - Biocompatible Materials/*chemical synthesis/chemistry
MH  - Drug Carriers/*chemical synthesis/chemistry
MH  - Drug Delivery Systems
MH  - Fluoresceins/*chemistry
MH  - Glass/*chemistry
MH  - Hydrogen-Ion Concentration
MH  - Maleates/chemistry
MH  - Maleic Anhydrides/chemistry
MH  - Molecular Probes/*chemical synthesis/chemistry
MH  - Propylamines
MH  - Silanes/chemistry
MH  - Stimulation, Chemical
EDAT- 2014/04/12 06:00
MHDA- 2015/08/01 06:00
CRDT- 2014/04/12 06:00
PHST- 2013/12/19 00:00 [received]
PHST- 2014/03/27 00:00 [accepted]
PHST- 2014/04/12 06:00 [entrez]
PHST- 2014/04/12 06:00 [pubmed]
PHST- 2015/08/01 06:00 [medline]
AID - 10.1007/s10856-014-5206-4 [doi]
PST - ppublish
SO  - J Mater Sci Mater Med. 2014 Oct;25(10):2243-53. doi: 10.1007/s10856-014-5206-4. 
      Epub 2014 Apr 11.