PMID- 24724742
OWN - NLM
STAT- MEDLINE
DCOM- 20151026
LR  - 20181202
IS  - 1468-1331 (Electronic)
IS  - 1351-5101 (Linking)
VI  - 21
IP  - 8
DP  - 2014 Aug
TI  - Identification of the major HHV-6 antigen recognized by cerebrospinal fluid IgG 
      in multiple sclerosis.
PG  - 1096-1101
LID - 10.1111/ene.12435 [doi]
AB  - BACKGROUND AND PURPOSE: Different data show an association between human 
      herpesvirus 6 (HHV-6) and multiple sclerosis (MS). Intrathecal anti-HHV-6 
      immunoglobulin G (IgG) was detected in MS patients, but the antigen recognized by 
      cerebrospinal fluid (CSF) IgG has not been characterized yet. Our objective was 
      to identify the HHV-6 antigens recognized by IgG present in the CSF of patients 
      with MS. METHODS: Cerebrospinal fluid IgG of 15 MS patients and eight patients 
      with other neurological diseases was purified on protein G Sepharose columns. 
      Purified IgG from every patient was linked to a CNBr-activated Sepharose 4B 
      column. Fifty micrograms of viral extract was applied to each column. Bound 
      proteins were eluted and analysed by SDS-PAGE and silver staining. The viral 
      protein was characterized by mass spectrometry. RESULTS: A protein of 150 kD was 
      eluted from CSF IgG columns of three of eight patients with primary progressive 
      MS and one of seven with relapsing-remitting MS. After digestion and mass 
      spectrometry analysis 10 peptides were found with 100% homology with the major 
      capsid protein of the HHV-6A. DISCUSSION: These findings confirm the presence of 
      anti-HHV-6 IgG in CSF of MS patients, particularly in progressive forms, and 
      identify major capsid protein as the major antigen recognized by CSF IgG from MS 
      patients.
CI  - (c) 2014 The Author(s) European Journal of Neurology (c) 2014 EAN.
FAU - Alenda, R
AU  - Alenda R
AD  - MS Unit, Departments of Immunology and Neurology, Hospital Ramon y Cajal, IRYCIS, 
      Madrid, Spain.
AD  - Red Espanola de Esclerosis Multiple, Madrid, Spain.
FAU - Alvarez-Lafuente, R
AU  - Alvarez-Lafuente R
AD  - Red Espanola de Esclerosis Multiple, Madrid, Spain.
AD  - Department of Neurology, Hospital Clinico San Carlos, IdISSC, Madrid, Spain.
FAU - Costa-Frossard, L
AU  - Costa-Frossard L
AD  - MS Unit, Departments of Immunology and Neurology, Hospital Ramon y Cajal, IRYCIS, 
      Madrid, Spain.
AD  - Red Espanola de Esclerosis Multiple, Madrid, Spain.
FAU - Arroyo, R
AU  - Arroyo R
AD  - Red Espanola de Esclerosis Multiple, Madrid, Spain.
AD  - Department of Neurology, Hospital Clinico San Carlos, IdISSC, Madrid, Spain.
FAU - Mirete, S
AU  - Mirete S
AD  - MS Unit, Departments of Immunology and Neurology, Hospital Ramon y Cajal, IRYCIS, 
      Madrid, Spain.
FAU - Alvarez-Cermeno, J C
AU  - Alvarez-Cermeno JC
AD  - MS Unit, Departments of Immunology and Neurology, Hospital Ramon y Cajal, IRYCIS, 
      Madrid, Spain.
AD  - Red Espanola de Esclerosis Multiple, Madrid, Spain.
AD  - Department of Medicine, University of Alcala, Alcala de Henares, Madrid, Spain.
FAU - Villar, L M
AU  - Villar LM
AD  - MS Unit, Departments of Immunology and Neurology, Hospital Ramon y Cajal, IRYCIS, 
      Madrid, Spain.
AD  - Red Espanola de Esclerosis Multiple, Madrid, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140412
PL  - England
TA  - Eur J Neurol
JT  - European journal of neurology
JID - 9506311
RN  - 0 (Antigens, Viral)
RN  - 0 (Capsid Proteins)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Antigens, Viral/*immunology
MH  - Capsid Proteins/*immunology
MH  - Female
MH  - Herpesvirus 6, Human/*immunology
MH  - Humans
MH  - Immunoglobulin G/cerebrospinal fluid/*immunology
MH  - Male
MH  - Middle Aged
MH  - Multiple Sclerosis/cerebrospinal fluid/*immunology/virology
MH  - Young Adult
OTO - NOTNLM
OT  - antibodies
OT  - demyelinating diseases
OT  - multiple sclerosis
OT  - neuroimmunology
EDAT- 2014/04/15 06:00
MHDA- 2015/10/27 06:00
CRDT- 2014/04/15 06:00
PHST- 2013/12/19 00:00 [received]
PHST- 2014/03/06 00:00 [accepted]
PHST- 2014/04/15 06:00 [entrez]
PHST- 2014/04/15 06:00 [pubmed]
PHST- 2015/10/27 06:00 [medline]
AID - 10.1111/ene.12435 [doi]
PST - ppublish
SO  - Eur J Neurol. 2014 Aug;21(8):1096-1101. doi: 10.1111/ene.12435. Epub 2014 Apr 12.