PMID- 24725362
OWN - NLM
STAT- MEDLINE
DCOM- 20141023
LR  - 20181202
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Linking)
VI  - 16
IP  - 7
DP  - 2014 Jul
TI  - Spatial distribution analyses of natural phyllosphere-colonizing bacteria on 
      Arabidopsis thaliana revealed by fluorescence in situ hybridization.
PG  - 2329-40
LID - 10.1111/1462-2920.12482 [doi]
AB  - Bacterial colonizers of the aerial parts of plants, or phyllosphere, have been 
      identified on a number of different plants using cultivation-dependent and 
      independent methods. However, the spatial distribution at the micrometer scale of 
      different main phylogenetic lineages is not well documented and mostly based on 
      fluorescence-tagged model strains. In this study, we developed and applied a 
      spatial explicit approach that allowed the use of fluorescence in situ 
      hybridization (FISH) to study bacterial phylloplane communities of 
      environmentally grown Arabidopsis thaliana. We found on average 5.4 x 10(6) 
      bacteria cm(-2) leaf surface and 1.5 x 10(8) bacteria g(-1) fresh weight. 
      Furthermore, we found that the total biomass in the phylloplane was normally 
      distributed. About 31% of the bacteria found in the phylloplane did not hybridize 
      to FISH probes but exhibited infrared autofluorescence indicative for aerobic 
      anoxygenic phototrophs. Four sets of FISH probes targeting Alphaproteobacteria, 
      Betaproteobacteria, Actinobacteria and Bacteroidetes were sufficient to identify 
      all other major contributors of the phylloplane community based on general 
      bacterial probing. Spatial aggregation patterns were observed for all 
      probe-targeted populations at distances up to 7 mum, with stronger tendencies to 
      co-aggregate for members of the same phylogenetic group. Our findings contribute 
      to a bottom-up description of leaf surface community composition.
CI  - (c) 2014 Society for Applied Microbiology and John Wiley & Sons Ltd.
FAU - Remus-Emsermann, Mitja N P
AU  - Remus-Emsermann MN
AD  - Institute of Microbiology, ETH Zurich, Vladimir-Prelog-Weg 4, 8093, Zurich, 
      Switzerland.
FAU - Lucker, Sebastian
AU  - Lucker S
FAU - Muller, Daniel B
AU  - Muller DB
FAU - Potthoff, Eva
AU  - Potthoff E
FAU - Daims, Holger
AU  - Daims H
FAU - Vorholt, Julia A
AU  - Vorholt JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140512
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
SB  - IM
MH  - Actinobacteria/classification/*physiology
MH  - Alphaproteobacteria/classification/*physiology
MH  - Arabidopsis/*microbiology
MH  - Bacterial Load
MH  - Bacteroidetes/classification/*physiology
MH  - Betaproteobacteria/classification/*physiology
MH  - In Situ Hybridization, Fluorescence
MH  - Microbial Consortia/physiology
MH  - Phylogeny
MH  - Plant Leaves/microbiology
EDAT- 2014/04/15 06:00
MHDA- 2014/10/24 06:00
CRDT- 2014/04/15 06:00
PHST- 2014/01/17 00:00 [received]
PHST- 2014/04/05 00:00 [accepted]
PHST- 2014/04/15 06:00 [entrez]
PHST- 2014/04/15 06:00 [pubmed]
PHST- 2014/10/24 06:00 [medline]
AID - 10.1111/1462-2920.12482 [doi]
PST - ppublish
SO  - Environ Microbiol. 2014 Jul;16(7):2329-40. doi: 10.1111/1462-2920.12482. Epub 
      2014 May 12.