PMID- 24725448
OWN - NLM
STAT- MEDLINE
DCOM- 20140710
LR  - 20181202
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 171
IP  - 1-2
DP  - 2014 Jun 25
TI  - Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal 
      ganglia of naturally infected cattle by polymerase chain reaction.
PG  - 182-8
LID - S0378-1135(14)00162-X [pii]
LID - 10.1016/j.vetmic.2014.03.012 [doi]
AB  - Establishment of latent infection within specific tissues in the host is a common 
      biological feature of the herpesviruses. In the case of bovine herpesvirus 2 
      (BoHV-2), latency is established in neuronal tissues, while bovine herpesvirus 4 
      (BoHV-4) and ovine herpesvirus 2 (OvHV-2) latent virus targets on cells of the 
      monocytic lineage. This study was conducted in quest of BoHV-2, BoHV-4 and OvHV-2 
      DNA in two hundred trigeminal ganglia (TG) specimens, derived from one hundred 
      clinically healthy cattle, majority of them naturally infected with bovine 
      herpesvirus 1 (BoHV-1) and bovine herpesvirus 5 (BoHV-5). Total DNA extracted 
      from ganglia was analyzed by polymerase chain reaction (PCR) designed to amplify 
      part of the genes coding for BoHV-2, and BoHV-4 glycoprotein B and, for OvHV-2, 
      the gene coding for phosphoribosylformylglycinamidine synthase-like protein. 
      BoHV-2 DNA was detected in TG samples of two (2%) and BoHV-4 DNA in nine (9%) of 
      the animals, whereas OvHV-2 DNA could not be detected in any of the TG DNA. The 
      two animals in which BoHV-2 DNA was identified were also co-infected with BoHV-1 
      and BoHV-5. Within the nine animals in which BoHV-4 DNA was detected, six were 
      also co-infected with BoHV-1 and BoHV-5. This report provides for the first time 
      evidence that viral DNA from BoHV-2 and BoHV-4 can be occasionally detected in TG 
      of naturally infected cattle. Likewise, in this report we provided for the first 
      time evidence that the co-infection of cattle with three distinct bovine 
      herpesviruses might be a naturally occurring phenomenon.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Campos, F S
AU  - Campos FS
AD  - Laboratory of Virology, Department of Microbiology, Immunology and Parasitology, 
      Institute of Basic Health Sciences, Federal University of Rio Grande do Sul 
      (UFRGS), Av. Sarmento Leite 500, Porto Alegre, CEP 90.050-170, Rio Grande do Sul 
      (RS), Brazil. Electronic address: camposvet@gmail.com.
FAU - Franco, A C
AU  - Franco AC
AD  - Laboratory of Virology, Department of Microbiology, Immunology and Parasitology, 
      Institute of Basic Health Sciences, Federal University of Rio Grande do Sul 
      (UFRGS), Av. Sarmento Leite 500, Porto Alegre, CEP 90.050-170, Rio Grande do Sul 
      (RS), Brazil.
FAU - Oliveira, M T
AU  - Oliveira MT
AD  - Laboratory of Virology, Department of Microbiology, Immunology and Parasitology, 
      Institute of Basic Health Sciences, Federal University of Rio Grande do Sul 
      (UFRGS), Av. Sarmento Leite 500, Porto Alegre, CEP 90.050-170, Rio Grande do Sul 
      (RS), Brazil.
FAU - Firpo, R
AU  - Firpo R
AD  - Laboratory of Virology, Department of Microbiology, Immunology and Parasitology, 
      Institute of Basic Health Sciences, Federal University of Rio Grande do Sul 
      (UFRGS), Av. Sarmento Leite 500, Porto Alegre, CEP 90.050-170, Rio Grande do Sul 
      (RS), Brazil.
FAU - Strelczuk, G
AU  - Strelczuk G
AD  - Laboratory of Virology, Department of Microbiology, Immunology and Parasitology, 
      Institute of Basic Health Sciences, Federal University of Rio Grande do Sul 
      (UFRGS), Av. Sarmento Leite 500, Porto Alegre, CEP 90.050-170, Rio Grande do Sul 
      (RS), Brazil.
FAU - Fontoura, F E
AU  - Fontoura FE
AD  - Laboratory of Virology, Department of Microbiology, Immunology and Parasitology, 
      Institute of Basic Health Sciences, Federal University of Rio Grande do Sul 
      (UFRGS), Av. Sarmento Leite 500, Porto Alegre, CEP 90.050-170, Rio Grande do Sul 
      (RS), Brazil.
FAU - Kulmann, M I R
AU  - Kulmann MI
AD  - Laboratory of Virology, Department of Microbiology, Immunology and Parasitology, 
      Institute of Basic Health Sciences, Federal University of Rio Grande do Sul 
      (UFRGS), Av. Sarmento Leite 500, Porto Alegre, CEP 90.050-170, Rio Grande do Sul 
      (RS), Brazil.
FAU - Maidana, S
AU  - Maidana S
AD  - Instituto de Virologia, Centro de Investigaciones en Ciencias Veterinarias y 
      Agronomicas (CICVyA), Instituto de tecnologia Agropecuaria (INTA), N. Repetto y 
      Los Reseros S/N, CC25 (B1712WAA), Castelar, Buenos Aires, Argentina.
FAU - Romera, S A
AU  - Romera SA
AD  - Instituto de Virologia, Centro de Investigaciones en Ciencias Veterinarias y 
      Agronomicas (CICVyA), Instituto de tecnologia Agropecuaria (INTA), N. Repetto y 
      Los Reseros S/N, CC25 (B1712WAA), Castelar, Buenos Aires, Argentina.
FAU - Spilki, F R
AU  - Spilki FR
AD  - Molecular Microbiology Laboratory, Institute of Health Sciences, Feevale 
      University, Rodovia RS-239 2755, Novo Hamburgo, CEP 93.352-000, Rio Grande do Sul 
      (RS), Brazil.
FAU - Silva, A D
AU  - Silva AD
AD  - Embrapa CNPSA, BR 153, Km 110, Post Box 21, Concordia, CEP 89.700-000, Santa 
      Catarina (SC), Brazil.
FAU - Hubner, S O
AU  - Hubner SO
AD  - Laboratory of Virology, Department of Preventive Veterinary Medicine, Faculty of 
      Veterinary Medicine, Federal University of Pelotas (UFPel), University Campus, 
      Post Box 354, Pelotas, CEP 96.010-900, Rio Grande do Sul (RS), Brazil.
FAU - Roehe, P M
AU  - Roehe PM
AD  - Laboratory of Virology, Department of Microbiology, Immunology and Parasitology, 
      Institute of Basic Health Sciences, Federal University of Rio Grande do Sul 
      (UFRGS), Av. Sarmento Leite 500, Porto Alegre, CEP 90.050-170, Rio Grande do Sul 
      (RS), Brazil; Institute for Veterinary Research "Desiderio Finamor" (IPVDF), 
      Estrada do Conde 6000, Eldorado do Sul, CEP 92.990-000, Rio Grande do Sul (RS), 
      Brazil.
LA  - eng
SI  - GENBANK/JQ958306
SI  - GENBANK/JQ958307
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140325
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cattle Diseases/*diagnosis/virology
MH  - Cell Line
MH  - Coinfection
MH  - DNA, Viral/genetics
MH  - Herpesviridae Infections/diagnosis/*veterinary/virology
MH  - Herpesvirus 1, Bovine/physiology
MH  - Herpesvirus 2, Bovine/genetics
MH  - Herpesvirus 4, Bovine/genetics
MH  - Herpesvirus 5, Bovine/physiology
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Polymerase Chain Reaction/*veterinary
MH  - Trigeminal Ganglion/*virology
OTO - NOTNLM
OT  - Bovine herpesvirus
OT  - Internal control
OT  - Latency
OT  - Ovine herpesvirus
OT  - PCR
EDAT- 2014/04/15 06:00
MHDA- 2014/07/11 06:00
CRDT- 2014/04/15 06:00
PHST- 2013/12/05 00:00 [received]
PHST- 2014/03/10 00:00 [revised]
PHST- 2014/03/12 00:00 [accepted]
PHST- 2014/04/15 06:00 [entrez]
PHST- 2014/04/15 06:00 [pubmed]
PHST- 2014/07/11 06:00 [medline]
AID - S0378-1135(14)00162-X [pii]
AID - 10.1016/j.vetmic.2014.03.012 [doi]
PST - ppublish
SO  - Vet Microbiol. 2014 Jun 25;171(1-2):182-8. doi: 10.1016/j.vetmic.2014.03.012. 
      Epub 2014 Mar 25.