PMID- 24725449
OWN - NLM
STAT- MEDLINE
DCOM- 20140710
LR  - 20181202
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 171
IP  - 1-2
DP  - 2014 Jun 25
TI  - Identification of Treponema pedis as the predominant Treponema species in porcine 
      skin ulcers by fluorescence in situ hybridization and high-throughput sequencing.
PG  - 122-31
LID - S0378-1135(14)00169-2 [pii]
LID - 10.1016/j.vetmic.2014.03.019 [doi]
AB  - Skin lesions often seen in pig production are of great animal welfare concern. To 
      study the potential role of Treponema bacteria in porcine skin ulcers, we 
      investigated the presence and distribution of these organisms in decubital 
      shoulder ulcers (n=51) and ear necroses (n=54) by fluorescence in situ 
      hybridization (FISH) and high-throughput sequencing. In addition, two cases of 
      facial ulcers and five cases of other skin ulcers were included in the study. 
      Samples from all 112 skin lesions and intact skin from pigs without skin ulcers 
      (n=14) were screened by FISH. Three different oligonucleotide probes targeting 
      16S rRNA were used, specific for domain bacterium, Treponema spp. and species T. 
      pedis. Screening showed that two cases each of facial and other ulcers, 35 (69%) 
      of shoulder ulcers and 32 (59%) of ear necroses were positive for Treponema spp. 
      T. pedis was the unequivocally, predominant species typically constituting more 
      than 90% of the treponemes in a lesion, assessed visually by microscopy. 
      Altogether, T. pedis was demonstrated in 69 of the 71 Treponema spp. positive 
      lesions. We conclude that Treponema spp. are frequently present and abundant in 
      various skin ulcers of pigs. The results from this study point toward an 
      important role of T. pedis as a secondary bacterial infection in porcine skin 
      ulcers, especially in severe and chronic lesions.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Karlsson, Frida
AU  - Karlsson F
AD  - Department of Clinical Sciences, Swedish University of Agricultural Sciences, 
      SE-75007 Uppsala, Sweden. Electronic address: frida.karlsson@slu.se.
FAU - Klitgaard, Kirstine
AU  - Klitgaard K
AD  - National Veterinary Institute, Technical University of Denmark, 1870 
      Frederiksberg, Denmark.
FAU - Jensen, Tim Kare
AU  - Jensen TK
AD  - National Veterinary Institute, Technical University of Denmark, 1870 
      Frederiksberg, Denmark.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140325
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - *In Situ Hybridization, Fluorescence
MH  - Oligonucleotide Probes/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sensitivity and Specificity
MH  - Skin Ulcer/microbiology/*veterinary
MH  - Swine
MH  - Swine Diseases/*diagnosis/*microbiology/pathology
MH  - Treponema/classification/*genetics
MH  - Treponemal Infections/diagnosis/microbiology/pathology/*veterinary
OTO - NOTNLM
OT  - Ear necrosis
OT  - FISH
OT  - Pig
OT  - Shoulder ulcer
OT  - Skin ulcer
OT  - Treponema
EDAT- 2014/04/15 06:00
MHDA- 2014/07/11 06:00
CRDT- 2014/04/15 06:00
PHST- 2014/02/03 00:00 [received]
PHST- 2014/03/07 00:00 [revised]
PHST- 2014/03/12 00:00 [accepted]
PHST- 2014/04/15 06:00 [entrez]
PHST- 2014/04/15 06:00 [pubmed]
PHST- 2014/07/11 06:00 [medline]
AID - S0378-1135(14)00169-2 [pii]
AID - 10.1016/j.vetmic.2014.03.019 [doi]
PST - ppublish
SO  - Vet Microbiol. 2014 Jun 25;171(1-2):122-31. doi: 10.1016/j.vetmic.2014.03.019. 
      Epub 2014 Mar 25.