PMID- 24726616
OWN - NLM
STAT- MEDLINE
DCOM- 20141218
LR  - 20220129
IS  - 1532-2823 (Electronic)
IS  - 0952-3278 (Linking)
VI  - 90
IP  - 6
DP  - 2014 Jun
TI  - Temporal changes in human skeletal muscle and blood lipid composition with fish 
      oil supplementation.
PG  - 199-206
LID - S0952-3278(14)00046-5 [pii]
LID - 10.1016/j.plefa.2014.03.001 [doi]
AB  - The aim of this study was to examine changes in the lipid profile of red blood 
      cells and muscle tissue along with the expression of anabolic signalling proteins 
      in human skeletal muscle. Following a 2-week control period, 10 healthy male 
      participants consumed 5 g d(-1) of fish oil (FO) for 4 weeks. Muscle biopsies and 
      venous blood samples were collected in the fasted state 2 weeks prior (W-2) and 
      immediately before (W0) the initiation of FO supplementation for internal 
      control. Muscle biopsies and venous blood samples were again obtained at week 1 
      (W1), 2 (W2) and 4 (W4) during FO supplementation for assessment of changes in 
      lipid composition and expression of anabolic signalling proteins. There was no 
      change in the composition of any lipid class between W-2 and W0 confirming 
      control. Following FO supplementation n-3 polyunsaturated fatty acid (n-3 PUFA) 
      muscle lipid composition was increased from W0 to W2 and continued to rise at W4. 
      n-3 PUFA blood lipid composition was increased from W0 to W1 and remained 
      elevated for the remaining time points. Total protein content of focal adhesion 
      kinase (FAK) increased from W0 to W4 whereas total mechanistic target of 
      rapamycin (mTOR) was increased from W0 at W1 with no further significant 
      increases at W2 and W4. These data show that FO supplementation results in 
      discordant changes in the n-3 PUFA composition of skeletal muscle compared to 
      blood that is associated with increases in total FAK content.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - McGlory, Chris
AU  - McGlory C
AD  - Exercise Metabolism Research Group, Department of Kinesiology, McMaster 
      University, Ontario, Canada; Health and Exercise Sciences Research Group, 
      University of Stirling, Scotland, UK. Electronic address: 
      Christopher.mcglory@stir.ac.uk.
FAU - Galloway, Stuart D R
AU  - Galloway SD
AD  - Health and Exercise Sciences Research Group, University of Stirling, Scotland, 
      UK.
FAU - Hamilton, D Lee
AU  - Hamilton DL
AD  - Health and Exercise Sciences Research Group, University of Stirling, Scotland, 
      UK.
FAU - McClintock, Craig
AU  - McClintock C
AD  - Health and Exercise Sciences Research Group, University of Stirling, Scotland, 
      UK.
FAU - Breen, Leigh
AU  - Breen L
AD  - Exercise Metabolism Research Group, Department of Kinesiology, McMaster 
      University, Ontario, Canada; School of Sport and Exercise Sciences, University of 
      Birmingham, UK.
FAU - Dick, James R
AU  - Dick JR
AD  - Institute of Aquaculture, University of Stirling, Scotland, UK.
FAU - Bell, John G
AU  - Bell JG
AD  - Institute of Aquaculture, University of Stirling, Scotland, UK.
FAU - Tipton, Kevin D
AU  - Tipton KD
AD  - Health and Exercise Sciences Research Group, University of Stirling, Scotland, 
      UK. Electronic address: k.d.tipton@stir.ac.uk.
LA  - eng
GR  - MR/K00414X/1/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140329
PL  - Scotland
TA  - Prostaglandins Leukot Essent Fatty Acids
JT  - Prostaglandins, leukotrienes, and essential fatty acids
JID - 8802730
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Anabolic Agents)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (EIF4EBP1 protein, human)
RN  - 0 (Fish Oils)
RN  - 0 (Lipids)
RN  - 0 (Phosphoproteins)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.10.2 (Focal Adhesion Kinase 1)
RN  - EC 2.7.10.2 (PTK2 protein, human)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/metabolism
MH  - Adolescent
MH  - Anabolic Agents/administration & dosage
MH  - Cell Cycle Proteins
MH  - *Dietary Supplements
MH  - Fish Oils/*administration & dosage
MH  - Focal Adhesion Kinase 1/metabolism
MH  - Humans
MH  - Lipids/*blood
MH  - Male
MH  - Muscle, Skeletal/drug effects/*metabolism
MH  - Phosphoproteins/metabolism
MH  - Protein Biosynthesis/drug effects
MH  - Ribosomal Protein S6 Kinases, 70-kDa/metabolism
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Young Adult
OTO - NOTNLM
OT  - FAK
OT  - Muscle
OT  - n-3 PUFA
EDAT- 2014/04/15 06:00
MHDA- 2014/12/19 06:00
CRDT- 2014/04/15 06:00
PHST- 2014/01/10 00:00 [received]
PHST- 2014/02/17 00:00 [revised]
PHST- 2014/03/19 00:00 [accepted]
PHST- 2014/04/15 06:00 [entrez]
PHST- 2014/04/15 06:00 [pubmed]
PHST- 2014/12/19 06:00 [medline]
AID - S0952-3278(14)00046-5 [pii]
AID - 10.1016/j.plefa.2014.03.001 [doi]
PST - ppublish
SO  - Prostaglandins Leukot Essent Fatty Acids. 2014 Jun;90(6):199-206. doi: 
      10.1016/j.plefa.2014.03.001. Epub 2014 Mar 29.