PMID- 24726860
OWN - NLM
STAT- MEDLINE
DCOM- 20150106
LR  - 20171116
IS  - 1879-0542 (Electronic)
IS  - 0165-2478 (Linking)
VI  - 160
IP  - 1
DP  - 2014 Jul
TI  - Day 3 Poly (I:C)-activated dendritic cells generated in CellGro for use in cancer 
      immunotherapy trials are fully comparable to standard Day 5 DCs.
PG  - 39-49
LID - S0165-2478(14)00056-X [pii]
LID - 10.1016/j.imlet.2014.03.010 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) are professional antigen-presenting cells that 
      are capable of inducing immune responses. DC-based vaccines are normally 
      generated using a standard 5- to 7-day protocol. To shorten the DC-based vaccine 
      production for use in cancer immunotherapy, we have developed a fast DC protocol 
      by comparing standard DCs (Day 5 DCs) and fast DCs (Day 3 DCs). METHODS: We 
      tested the generation of Day 5 versus Day 3 DCs using CellGro media and 
      subsequent activation by two activation stimuli: Poly (I:C) and LPS. We evaluated 
      DC morphology, viability, phagocyte activity, cytokine production and ability to 
      stimulate antigen-specific T cells. RESULTS: Day 5 and Day 3 DCs exhibited 
      similar phagocytic capacity. Poly (I:C)-activated Day 5 DCs expressed higher 
      levels of the costimulatory and surface molecules CD80, CD86 and HLA-DR compared 
      to Poly (I:C)-activated Day 3 DCs. Nevertheless, LPS-activated Day 5 and Day 3 
      DCs were phenotypically similar. Cytokine production was generally stronger when 
      LPS was used as the maturation stimulus, and there were no significant 
      differences between Day 5 and Day 3 DCs. Importantly, Day 5 and Day 3 DCs were 
      able to generate comparable numbers of antigen-specific CD8(+) T cells. The 
      number of Tregs induced by Day 5 and Day 3 DCs was also comparable. CONCLUSION: 
      We identified monocyte-derived DCs generated in CellGro for 3 days and activated 
      using Poly (I:C) similarly potent in most functional aspects as DCs produced by 
      the standard 5 day protocol. These results provide the rationale for the 
      evaluation of faster protocols for DC generation in clinical trials.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Truxova, Iva
AU  - Truxova I
AD  - Department of Immunology, Charles University, Second Faculty of Medicine and 
      University Hospital Motol, Prague, Czech Republic; Sotio, Prague, Czech Republic.
FAU - Pokorna, Katerina
AU  - Pokorna K
AD  - Sotio, Prague, Czech Republic.
FAU - Kloudova, Kamila
AU  - Kloudova K
AD  - Sotio, Prague, Czech Republic.
FAU - Partlova, Simona
AU  - Partlova S
AD  - Department of Immunology, Charles University, Second Faculty of Medicine and 
      University Hospital Motol, Prague, Czech Republic; Sotio, Prague, Czech Republic.
FAU - Spisek, Radek
AU  - Spisek R
AD  - Department of Immunology, Charles University, Second Faculty of Medicine and 
      University Hospital Motol, Prague, Czech Republic; Sotio, Prague, Czech Republic.
FAU - Fucikova, Jitka
AU  - Fucikova J
AD  - Department of Immunology, Charles University, Second Faculty of Medicine and 
      University Hospital Motol, Prague, Czech Republic; Sotio, Prague, Czech Republic. 
      Electronic address: jitka.fucikova@fnmotol.cz.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140412
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Cancer Vaccines)
RN  - 0 (Cytokines)
RN  - O84C90HH2L (Poly I-C)
SB  - IM
MH  - Antigen-Presenting Cells/drug effects/immunology/metabolism
MH  - Cancer Vaccines/immunology
MH  - *Cell Culture Techniques
MH  - Cell Differentiation/drug effects/immunology
MH  - Cell Survival
MH  - Cytokines/biosynthesis
MH  - Dendritic Cells/cytology/drug effects/*immunology/metabolism
MH  - Humans
MH  - Immunophenotyping
MH  - Immunotherapy
MH  - Neoplasms/*immunology/metabolism/therapy
MH  - Phagocytosis/immunology
MH  - Phenotype
MH  - Poly I-C/*immunology/pharmacology
MH  - T-Cell Antigen Receptor Specificity/immunology
MH  - T-Lymphocyte Subsets/immunology/metabolism
MH  - Time Factors
OTO - NOTNLM
OT  - Cancer immunotherapy
OT  - Dendritic cell
OT  - Fast generation
OT  - Monocyte
OT  - Vaccine
EDAT- 2014/04/15 06:00
MHDA- 2015/01/07 06:00
CRDT- 2014/04/15 06:00
PHST- 2013/11/08 00:00 [received]
PHST- 2014/03/19 00:00 [revised]
PHST- 2014/03/26 00:00 [accepted]
PHST- 2014/04/15 06:00 [entrez]
PHST- 2014/04/15 06:00 [pubmed]
PHST- 2015/01/07 06:00 [medline]
AID - S0165-2478(14)00056-X [pii]
AID - 10.1016/j.imlet.2014.03.010 [doi]
PST - ppublish
SO  - Immunol Lett. 2014 Jul;160(1):39-49. doi: 10.1016/j.imlet.2014.03.010. Epub 2014 
      Apr 12.