PMID- 24728343
OWN - NLM
STAT- MEDLINE
DCOM- 20141209
LR  - 20190221
IS  - 1791-2423 (Electronic)
IS  - 1019-6439 (Linking)
VI  - 44
IP  - 6
DP  - 2014 Jun
TI  - High-resolution detection of recurrent aberrations in lung adenocarcinomas by 
      array comparative genomic hybridization and expression analysis of selective 
      genes by quantitative PCR.
PG  - 2068-76
LID - 10.3892/ijo.2014.2384 [doi]
AB  - Genomic abnormalities are the hallmark of cancers and may harbor potential 
      candidate genes important for cancer development and progression. We performed 
      array comparative genomic hybridization (array CGH) on 36 cases of primary lung 
      adenocarcinoma (AD) using an array containing 2621 BAC or PAC clones spanning the 
      genome at an average interval of 1 Mb. Array CGH identified the commonest 
      aberrations consisting of DNA gains within 1p, 1q, 5p, 5q, 7p, 7q, 8q, 11q, 12p, 
      13q, 16p, 17q, 20q, and losses with 6q, 9p, 10q and 18q. High-level copy gains 
      involved mainly 7p21-p15 and 20q13.3. Dual color fluorescence in situ 
      hybridization (FISH) was performed on a selective locus for validation of array 
      CGH results. Genomic aberrations were compared with different clinicopathological 
      features and a trend of higher number of aberrations in tumors with aggressive 
      phenotypes and current tobacco exposure was identified. According to array CGH 
      data, 23 candidate genes were selected for quantitative PCR (qPCR) analysis. The 
      concordance observed between the genomic and expression changes in most of the 
      genes suggested that they could be candidate cancer-related genes that 
      contributed to the development of lung AD.
FAU - Zhu, Hong
AU  - Zhu H
AD  - Department of Pathology, Beijing Friendship Hospital, Capital Medical University, 
      Beijing 100050, P.R. China.
FAU - Wong, Maria Pik
AU  - Wong MP
AD  - Department of Pathology, The University of Hong Kong, Hong Kong, SAR, P.R. China.
FAU - Tin, Vicky
AU  - Tin V
AD  - Department of Pathology, The University of Hong Kong, Hong Kong, SAR, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20140411
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
SB  - IM
MH  - Adenocarcinoma/*genetics/*pathology
MH  - Adenocarcinoma of Lung
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Aberrations
MH  - Comparative Genomic Hybridization
MH  - Female
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic
MH  - Genome, Human
MH  - Humans
MH  - Lung Neoplasms/*genetics/*pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Tobacco Use/adverse effects
EDAT- 2014/04/15 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/04/15 06:00
PHST- 2014/01/26 00:00 [received]
PHST- 2014/03/28 00:00 [accepted]
PHST- 2014/04/15 06:00 [entrez]
PHST- 2014/04/15 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - 10.3892/ijo.2014.2384 [doi]
PST - ppublish
SO  - Int J Oncol. 2014 Jun;44(6):2068-76. doi: 10.3892/ijo.2014.2384. Epub 2014 Apr 
      11.