PMID- 24728996
OWN - NLM
STAT- MEDLINE
DCOM- 20140804
LR  - 20211021
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 42
IP  - 10
DP  - 2014 Jun
TI  - Telomere length kinetics assay (TELKA) sorts the telomere length maintenance 
      (tlm) mutants into functional groups.
PG  - 6314-25
LID - 10.1093/nar/gku267 [doi]
AB  - Genome-wide systematic screens in yeast have uncovered a large gene network (the 
      telomere length maintenance network or TLM), encompassing more than 400 genes, 
      which acts coordinatively to maintain telomere length. Identifying the genes was 
      an important first stage; the next challenge is to decipher their mechanism of 
      action and to organize then into functional groups or pathways. Here we present a 
      new telomere-length measuring program, TelQuant, and a novel assay, telomere 
      length kinetics assay, and use them to organize tlm mutants into functional 
      classes. Our results show that a mutant defective for the relatively unknown MET7 
      gene has the same telomeric kinetics as mutants defective for the ribonucleotide 
      reductase subunit Rnr1, in charge of the limiting step in dNTP synthesis, or for 
      the Ku heterodimer, a well-established telomere complex. We confirm the epistatic 
      relationship between the mutants and show that physical interactions exist 
      between Rnr1 and Met7. We also show that Met7 and the Ku heterodimer affect dNTP 
      formation, and play a role in non-homologous end joining. Thus, our telomere 
      kinetics assay uncovers new functional groups, as well as complex genetic 
      interactions between tlm mutants.
CI  - (c) The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic 
      Acids Research.
FAU - Rubinstein, Linda
AU  - Rubinstein L
AD  - Department of Molecular Microbiology and Biotechnology, Tel Aviv University, 
      Ramat Aviv 69978, Israel.
FAU - Ungar, Lior
AU  - Ungar L
AD  - Department of Molecular Microbiology and Biotechnology, Tel Aviv University, 
      Ramat Aviv 69978, Israel.
FAU - Harari, Yaniv
AU  - Harari Y
AD  - Department of Molecular Microbiology and Biotechnology, Tel Aviv University, 
      Ramat Aviv 69978, Israel.
FAU - Babin, Vera
AU  - Babin V
AD  - Department of Molecular Microbiology and Biotechnology, Tel Aviv University, 
      Ramat Aviv 69978, Israel.
FAU - Ben-Aroya, Shay
AU  - Ben-Aroya S
AD  - Faculty of Life Sciences Bar-Ilan University, Ramat-Gan, Israel.
FAU - Merenyi, Gabor
AU  - Merenyi G
AD  - Department of Medical Biochemistry and Biophysics and Laboratory for Molecular 
      Infection Medicine Sweden (MIMS), Umea University, Umea 901 87, Sweden.
FAU - Marjavaara, Lisette
AU  - Marjavaara L
AD  - Department of Medical Biochemistry and Biophysics and Laboratory for Molecular 
      Infection Medicine Sweden (MIMS), Umea University, Umea 901 87, Sweden.
FAU - Chabes, Andrei
AU  - Chabes A
AD  - Department of Medical Biochemistry and Biophysics and Laboratory for Molecular 
      Infection Medicine Sweden (MIMS), Umea University, Umea 901 87, Sweden.
FAU - Kupiec, Martin
AU  - Kupiec M
AD  - Department of Molecular Microbiology and Biotechnology, Tel Aviv University, 
      Ramat Aviv 69978, Israel martin@post.tau.ac.il.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140411
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (YKU70 protein, S cerevisiae)
RN  - 0 (high affinity DNA-binding factor, S cerevisiae)
RN  - EC 1.17.4.- (Ribonucleotide Reductases)
RN  - EC 1.17.4.- (Rnr1 protein, S cerevisiae)
RN  - EC 6.3.2.- (Peptide Synthases)
RN  - EC 6.3.2.17 (folylpolyglutamate synthetase)
SB  - IM
MH  - Blotting, Southern
MH  - DNA End-Joining Repair
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - *Mutation
MH  - Peptide Synthases/genetics/metabolism
MH  - Ribonucleotide Reductases/metabolism
MH  - Saccharomyces cerevisiae Proteins/genetics/metabolism
MH  - Telomere Homeostasis/*genetics
PMC - PMC4041441
EDAT- 2014/04/15 06:00
MHDA- 2014/08/05 06:00
PMCR- 2014/04/11
CRDT- 2014/04/15 06:00
PHST- 2014/04/15 06:00 [entrez]
PHST- 2014/04/15 06:00 [pubmed]
PHST- 2014/08/05 06:00 [medline]
PHST- 2014/04/11 00:00 [pmc-release]
AID - gku267 [pii]
AID - 10.1093/nar/gku267 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 2014 Jun;42(10):6314-25. doi: 10.1093/nar/gku267. Epub 2014 
      Apr 11.