PMID- 24729660
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 0924-4247 (Print)
IS  - 0924-4247 (Linking)
VI  - 195
DP  - 2013 Jun 1
TI  - Nucleic Acid Isolation and Enrichment on a Microchip.
PG  - 183-190
AB  - This paper presents a microchip that isolates and enriches target-binding 
      single-stranded DNA (ssDNA) from a randomized DNA mixture using a combination of 
      solid-phase extraction and electrophoresis. Strands of ssDNA in a randomized 
      mixture are captured via specific binding onto target-functionalized microbeads 
      in a microchamber. The strands are further separated from impurities and enriched 
      on-chip via electrophoresis. The microchip consists of two microchambers that are 
      connected by a channel filled with agarose gel. In the isolation chamber, beads 
      functionalized with human immunoglobulin E (IgE) are retained by a weir 
      structure. An integrated heater elevates the temperature in the chamber to elute 
      desired ssDNA from the beads, and electrophoretic transport of the DNA through 
      the gel to the second chamber is accomplished by applying an electric potential 
      difference between the two chambers. Experimental results show that ssDNA 
      expressing binding affinity to IgE was captured and enriched from a sample of 
      ssDNA with random sequences, demonstrating the potential of the microchip to 
      enhance the sensitivity of ssDNA detection methods in dilute and complex 
      biological samples.
FAU - Kim, Jinho
AU  - Kim J
AD  - Department of Mechanical Engineering, Columbia University, New York, NY 10027.
FAU - Hilton, John P
AU  - Hilton JP
AD  - Department of Mechanical Engineering, Columbia University, New York, NY 10027.
FAU - Yang, Kyung A
AU  - Yang KA
AD  - Division of Clinical Pharmacology and Experimental Therapeutics, Department of 
      Medicine, Columbia University, New York, NY 10032.
FAU - Pei, Renjun
AU  - Pei R
AD  - Division of Clinical Pharmacology and Experimental Therapeutics, Department of 
      Medicine, Columbia University, New York, NY 10032.
FAU - Stojanovic, Milan
AU  - Stojanovic M
AD  - Division of Clinical Pharmacology and Experimental Therapeutics, Department of 
      Medicine, Columbia University, New York, NY 10032.
FAU - Lin, Qiao
AU  - Lin Q
AD  - Department of Mechanical Engineering, Columbia University, New York, NY 10027.
LA  - eng
GR  - R21 RR025816/RR/NCRR NIH HHS/United States
GR  - RC2 CA147925/CA/NCI NIH HHS/United States
PT  - Journal Article
PL  - Switzerland
TA  - Sens Actuators A Phys
JT  - Sensors and actuators. A, Physical
JID - 101137102
PMC - PMC3979544
MID - NIHMS402323
OTO - NOTNLM
OT  - Solid-phase extraction
OT  - electrophoresis
OT  - human immunoglobulin E
OT  - microchannel
OT  - ssDNA
EDAT- 2014/04/15 06:00
MHDA- 2014/04/15 06:01
PMCR- 2014/06/01
CRDT- 2014/04/15 06:00
PHST- 2014/04/15 06:00 [entrez]
PHST- 2014/04/15 06:00 [pubmed]
PHST- 2014/04/15 06:01 [medline]
PHST- 2014/06/01 00:00 [pmc-release]
AID - 10.1016/j.sna.2012.07.022 [doi]
PST - ppublish
SO  - Sens Actuators A Phys. 2013 Jun 1;195:183-190. doi: 10.1016/j.sna.2012.07.022.