PMID- 24730417
OWN - NLM
STAT- MEDLINE
DCOM- 20141208
LR  - 20211203
IS  - 1365-2826 (Electronic)
IS  - 0953-8194 (Linking)
VI  - 26
IP  - 5
DP  - 2014 May
TI  - 17alpha-Oestradiol-induced neuroprotection in the brain of spontaneously hypertensive 
      rats.
PG  - 310-20
LID - 10.1111/jne.12151 [doi]
AB  - 17beta-oestradiol is a powerful neuroprotective factor for the brain abnormalities 
      of spontaneously hypertensive rats (SHR). 17alpha-Oestradiol, a nonfeminising isomer 
      showing low affinity for oestrogen receptors, is also endowed with 
      neuroprotective effects in vivo and in vitro. We therefore investigated whether 
      treatment with 17alpha-oestradiol prevented pathological changes of the hippocampus 
      and hypothalamus of SHR. We used 20-week-old male SHR with a blood pressure of 
      approximately 170 mmHg receiving s.c. a single 800 mug pellet of 17alpha-oestradiol 
      dissolved in cholesterol or vehicle only for 2 weeks Normotensive Wistar-Kyoto 
      (WKY) rats were used as controls. 17alpha-Oestradiol did not modify blood pressure, 
      serum prolactin, 17beta-oestradiol levels or the weight of the testis and pituitary 
      of SHR. In the brain, we analysed steroid effects on hippocampus Ki67+ 
      proliferating cells, doublecortin (DCX) positive neuroblasts, glial fibrillary 
      acidic protein (GFAP)+ astrocyte density, aromatase immunostaining and 
      brain-derived neurotrophic factor (BDNF) mRNA. In the hypothalamus, we determined 
      arginine vasopressin (AVP) mRNA. Treatment of SHR with 17alpha-oestradiol enhanced 
      the number of Ki67+ in the subgranular zone and DCX+ cells in the inner granule 
      cell layer of the dentate gyrus, increased BDNF mRNA in the CA1 region and gyrus 
      dentatus, decreased GFAP+ astrogliosis in the CA1 subfield, and decreased 
      hypothalamic AVP mRNA. Aromatase expression was unmodified. By contrast to SHR, 
      normotensive WKY rats were unresponsive to 17alpha-oestradiol. These data indicate a 
      role for 17alpha-oestradiol as a protective factor for the treatment of hypertensive 
      encephalopathy. Furthermore, 17alpha-oestradiol is weakly oestrogenic in the 
      periphery and can be used in males.
CI  - (c) 2014 British Society for Neuroendocrinology.
FAU - Pietranera, L
AU  - Pietranera L
AD  - Laboratory of Neuroendocrine Biochemistry, Instituto de Biologia y Medicina 
      Experimental, Buenos Aires, Argentina; Department of Human Biochemistry, Faculty 
      of Medicine, University of Buenos Aires, Buenos Aires, Argentina.
FAU - Brocca, M E
AU  - Brocca ME
FAU - Roig, P
AU  - Roig P
FAU - Lima, A
AU  - Lima A
FAU - Garcia-Segura, L M
AU  - Garcia-Segura LM
FAU - De Nicola, A F
AU  - De Nicola AF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neuroendocrinol
JT  - Journal of neuroendocrinology
JID - 8913461
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Dcx protein, rat)
RN  - 0 (Doublecortin Protein)
RN  - 0 (Neuroprotective Agents)
RN  - 113-79-1 (Arginine Vasopressin)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Arginine Vasopressin/metabolism
MH  - Blood Pressure/drug effects
MH  - Brain/*drug effects
MH  - Brain Chemistry/drug effects
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Doublecortin Protein
MH  - Estradiol/*pharmacology
MH  - Gliosis/pathology
MH  - Male
MH  - Neurogenesis/drug effects
MH  - Neuroprotective Agents/*pharmacology
MH  - Rats
MH  - Rats, Inbred SHR
MH  - Rats, Inbred WKY
OTO - NOTNLM
OT  - hippocampus
OT  - hypertension
OT  - hypothalamus
OT  - oestrogens
EDAT- 2014/04/16 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/04/16 06:00
PHST- 2013/12/02 00:00 [received]
PHST- 2014/02/20 00:00 [revised]
PHST- 2014/03/16 00:00 [accepted]
PHST- 2014/04/16 06:00 [entrez]
PHST- 2014/04/16 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - 10.1111/jne.12151 [doi]
PST - ppublish
SO  - J Neuroendocrinol. 2014 May;26(5):310-20. doi: 10.1111/jne.12151.