PMID- 24731421
OWN - NLM
STAT- MEDLINE
DCOM- 20150529
LR  - 20181202
IS  - 1002-0098 (Print)
IS  - 1002-0098 (Linking)
VI  - 49
IP  - 2
DP  - 2014 Feb
TI  - [Expression of voltage gated sodium channel Nav1.9 in experimental pulpal lesions 
      in the rats].
PG  - 95-100
AB  - OBJECTIVE: To investigate the relationship between pulpitis pain and 
      voltage-gated sodium channel (Nav1.9) by detecting the expression of Nav1.9 at 
      different time points of the rat pulpal lesion model. METHODS: Thirty-six SD 
      pulpal lesions rat models were divided into three experimental groups, 1 d (n = 
      12), 3 d (n = 12) and 5 d group(n = 12).Normal SD rats served as control(n = 12). 
      Tumor necrosis factor-alpha (TNF-alpha) and Nav1.9 mRNA expressions were evaluated by 
      reverse transcription PCR (RT-PCR) .Nav1.9 protein expressions were analyzed by 
      enzyme-linked immunosorbent assay (ELISA) and Western blotting. RESULTS: The 
      expression of TNF-alpha in the experimental group (1 d:0.514 +/- 0.098, 3 d:0.739 +/- 
      0.104, 5 d:1.238 +/- 0.082) was higher than those in the control group (0.147 +/- 
      0.016) (P < 0.01). Nav1.9 mRNA was up-regulated markedly in experimental groups 
      (1 d: 0.296 +/- 0.038, 3 d:0.409 +/- 0.013, 5 d: 0.487 +/- 0.028) , compare with 
      control group (0.223 +/- 0.020) (P < 0.05). The ELISA results revealed that the 
      level of Nav1.9 in control pulp tissue was (4.013 +/- 0.292) microg/L, in painful pulp 
      tissue of 1 d group was (5.143 +/- 0.101) microg/L, in painful pulp tissue of 3 d group 
      was (5.835 +/- 0.088) microg/L and in painful pulp tissue of 5 d group was (6.307 +/- 
      0.137) microg/L (P < 0.05). Western blotting showed the expression of Nav1.9 in 
      experimental groups (1 d: 0.106 +/- 0.007, 3 d:0.170 +/- 0.013, 5 d:0.238 +/- 0.004) 
      was up-regulated significantly compared with control group (0.073 +/- 0.004)(P < 
      0.05). CONCLUSIONS: The level of Nav1.9 had a significant increase in painful 
      pulp tissue. Moreover, with the degree of pain aggravation, the expression of 
      Nav1.9 increased in pulp tissue.It suggests that Nav1.9 may play an important 
      role in the development of pulpitis pain.
FAU - Xu, Le
AU  - Xu L
AD  - Department of Preventive Dentistry, Stomatologic Hospital and College, Anhui 
      Medical University & Key Laboratory of Oral Diseases Research of Anhui Province, 
      Hefei 230032, China.
FAU - Zhu, Xinwei
AU  - Zhu X
AD  - Department of Preventive Dentistry, Stomatologic Hospital and College, Anhui 
      Medical University & Key Laboratory of Oral Diseases Research of Anhui Province, 
      Hefei 230032, China.
FAU - Chen, Qingfeng
AU  - Chen Q
AD  - Department of Preventive Dentistry, Stomatologic Hospital and College, Anhui 
      Medical University & Key Laboratory of Oral Diseases Research of Anhui Province, 
      Hefei 230032, China.
FAU - Hu, Yuanping
AU  - Hu Y
AD  - Department of Preventive Dentistry, Stomatologic Hospital and College, Anhui 
      Medical University & Key Laboratory of Oral Diseases Research of Anhui Province, 
      Hefei 230032, China.
FAU - Zhu, Ling
AU  - Zhu L
AD  - Department of Preventive Dentistry, Stomatologic Hospital and College, Anhui 
      Medical University & Key Laboratory of Oral Diseases Research of Anhui Province, 
      Hefei 230032, China.
FAU - Jiang, Yong
AU  - Jiang Y
AD  - Department of Preventive Dentistry, Stomatologic Hospital and College, Anhui 
      Medical University & Key Laboratory of Oral Diseases Research of Anhui Province, 
      Hefei 230032, China. Email: j6263@163.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Kou Qiang Yi Xue Za Zhi
JT  - Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese 
      journal of stomatology
JID - 8711066
RN  - 0 (NAV1.9 Voltage-Gated Sodium Channel)
RN  - 0 (RNA, Messenger)
RN  - 0 (Scn11a protein, rat)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Dental Pulp/*metabolism/pathology
MH  - NAV1.9 Voltage-Gated Sodium Channel/*metabolism
MH  - Pain
MH  - Pulpitis
MH  - RNA, Messenger
MH  - Rats
MH  - Tumor Necrosis Factor-alpha/*metabolism
MH  - Up-Regulation
EDAT- 2014/04/16 06:00
MHDA- 2015/05/30 06:00
CRDT- 2014/04/16 06:00
PHST- 2014/04/16 06:00 [entrez]
PHST- 2014/04/16 06:00 [pubmed]
PHST- 2015/05/30 06:00 [medline]
PST - ppublish
SO  - Zhonghua Kou Qiang Yi Xue Za Zhi. 2014 Feb;49(2):95-100.