PMID- 24733606
OWN - NLM
STAT- MEDLINE
DCOM- 20151015
LR  - 20181202
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 115
IP  - 9
DP  - 2014 Sep
TI  - MicroRNA-122 overexpression promotes hepatic differentiation of human adipose 
      tissue-derived stem cells.
PG  - 1582-93
LID - 10.1002/jcb.24822 [doi]
AB  - MicroRNAs are the regulatory molecules in post-transcriptional regulation of gene 
      expression, which affect diverse biological processes and have been found to play 
      important roles in regulating stem cell character in plants and animals. The aim 
      of this study was to identify the role of miR-122 during hepatic differentiation 
      of human adipose tissue-derived stem cells (hADSCs), and also to investigate 
      whether overexpression of miR-122 could enhance differentiation of hADSCs toward 
      functional hepatocyte-like cells without any extrinsic factor. To investigate 
      this, the level of miR-122 was monitored by quantitative real-time PCR (qRT-PCR) 
      at specific time intervals following hepatic differentiation of hADSCs using 
      growth factors. For the next step, lentiviral transduction was applied to 
      overexpress miR-122 in hADSCs for up to 21 days. Hepatic functionality was 
      evaluated by analyzing specific hepatocyte genes and biochemical markers at 
      different time points of differentiation induction. The qRT-PCR results revealed 
      that miR-122 was upregulated during hepatic differentiation of hADSCs. 
      Additionally, the stable miR-122 overexpression in hADSCs resulted in increased 
      expression of specific hepatocyte markers such as ALB, AFP, CK18, CK19, and HNF4a 
      compared with the negative control cells. Moreover, urea and albumin production 
      as well as glycogen deposits were observed in the treated cells. Therefore, our 
      findings demonstrate that the hepatic differentiation process could be improved 
      by the overexpression of miR-122 in hADSCs, making it a potential therapeutic 
      resource for liver disorders.
CI  - (c) 2014 Wiley Periodicals, Inc.
FAU - Davoodian, Nahid
AU  - Davoodian N
AD  - Department of Clinical Biochemistry, Faculty of Medical Science, Tarbiat Modares 
      University, Tehran, Iran.
FAU - Lotfi, Abbas S
AU  - Lotfi AS
FAU - Soleimani, Masoud
AU  - Soleimani M
FAU - Mowla, Seyed Javad
AU  - Mowla SJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Biomarkers)
RN  - 0 (MIRN122 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Adipose Tissue/*cytology/metabolism
MH  - Biomarkers/metabolism
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Dexamethasone
MH  - Gene Expression Regulation
MH  - Hepatocyte Growth Factor/metabolism
MH  - Hepatocytes/*cytology/physiology
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology
MH  - MicroRNAs/*genetics
OTO - NOTNLM
OT  - HEPATIC DIFFERENTIATION
OT  - HUMAN ADIPOSE TISSUE-DERIVED STEM CELLS
OT  - MicroRNA
OT  - miR-122
EDAT- 2014/04/16 06:00
MHDA- 2015/10/16 06:00
CRDT- 2014/04/16 06:00
PHST- 2014/01/27 00:00 [received]
PHST- 2014/04/11 00:00 [accepted]
PHST- 2014/04/16 06:00 [entrez]
PHST- 2014/04/16 06:00 [pubmed]
PHST- 2015/10/16 06:00 [medline]
AID - 10.1002/jcb.24822 [doi]
PST - ppublish
SO  - J Cell Biochem. 2014 Sep;115(9):1582-93. doi: 10.1002/jcb.24822.