PMID- 2473769
OWN - NLM
STAT- MEDLINE
DCOM- 19890831
LR  - 20161123
IS  - 0724-6803 (Print)
IS  - 0724-6803 (Linking)
VI  - 3
IP  - 4
DP  - 1987
TI  - Activation of murine B cells with Salmonella typhimurium mitogen (STM), 
      lipopolysaccharide (LPS), and dextran sulfate (DxS). I. Cell-cycle analysis and 
      induction of cytoplasmic immunoglobulin.
PG  - 215-9
AB  - There have been two recent reports concerning a B cell-specific mitogen that 
      induces proliferation, but not differentiation of rat and human B cells. This 
      mitogen, which is derived from Salmonella typhimurium (STM), appears to be 
      providing the signals required for anti-immunoglobulin-treated (anti-Ig) B cells 
      to enter cycle and divide, but may not be inducing responsiveness to B cell 
      differentiation factors (BCDF). In this report, we have compared STM to the other 
      known murine B cell polyclonal activators: lipopolysaccharide (LPS), dextran 
      sulfate (DxS), and the combination of LPS/DxS. STM was the most potent stimulus 
      of B cell proliferation as determined by uptake of 3H-thymidine, viable cell 
      numbers and cell cycle analysis utilizing acridine orange (AO). STM did not 
      induce significant proliferation of murine T lymphocytes. In addition, the 
      proliferative effect of STM on B cells shows minimal, if any, macrophage 
      dependence. However, in contrast to its effect on human and rat B cells, STM 
      induces differentiation of murine B cells. The levels of cytoplasmic Ig induced 
      by STM are equivalent or greater to those induced by LPS/DxS. Thus, in the murine 
      system, STM will be useful as a polyclonal activator which induces proliferation 
      and differentiation of the vast majority of the B cell population without 
      stringent accessory cell requirements.
FAU - Brooks, K H
AU  - Brooks KH
AD  - Department of Microbiology, University of Texas Health Science Center, 
      Southwestern Medical School, Dallas 75235.
FAU - Vitetta, E S
AU  - Vitetta ES
LA  - eng
GR  - AI-11851/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Mol Cell Immunol
JT  - The Journal of molecular and cellular immunology : JMCI
JID - 8405005
RN  - 0 (Dextrans)
RN  - 0 (Immunoglobulins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Mitogens)
RN  - 9042-14-2 (Dextran Sulfate)
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/cytology/*immunology
MH  - Cell Cycle
MH  - Cytoplasm/immunology
MH  - Dextran Sulfate
MH  - Dextrans/pharmacology
MH  - Female
MH  - Immunoglobulins/*biosynthesis
MH  - In Vitro Techniques
MH  - Lipopolysaccharides/pharmacology
MH  - *Lymphocyte Activation
MH  - Mice
MH  - Mitogens/pharmacology
MH  - Salmonella typhimurium/immunology
EDAT- 1987/01/01 00:00
MHDA- 1987/01/01 00:01
CRDT- 1987/01/01 00:00
PHST- 1987/01/01 00:00 [pubmed]
PHST- 1987/01/01 00:01 [medline]
PHST- 1987/01/01 00:00 [entrez]
PST - ppublish
SO  - J Mol Cell Immunol. 1987;3(4):215-9.