PMID- 24738966
OWN - NLM
STAT- MEDLINE
DCOM- 20141124
LR  - 20181202
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 117
IP  - 2
DP  - 2014 Aug
TI  - Microbial community structure of wastewater treatment subjected to high mortality 
      rate due to ozonation of return activated sludge.
PG  - 587-96
LID - 10.1111/jam.12523 [doi]
AB  - AIMS: This study investigated the effects of return activated sludge (RAS) 
      ozonation, on the bacterial community structure of pilot-scale wastewater 
      treatment systems. METHODS AND RESULTS: Two parallel activated sludge reactors 
      were operated to treat real municipal wastewater for 98 days. The RAS of one of 
      the reactors was subjected to increasing doses of ozone during the experimental 
      period, which resulted in higher reduction in biosolids waste production and 
      higher bacterial growth rate. The bacterial community structures were 
      investigated by 16S rRNA gene amplicon high-throughput pyrosequencing and 
      fluorescence in situ hybridization (FISH). The structures remained highly similar 
      throughout the experiment despite the ozone treatment. Comparative analyses 
      between pyrosequencing and FISH revealed clear discrepancies in the proportion of 
      some bacterial populations. CONCLUSIONS: The results suggest that RAS ozonation 
      is not a main environmental factor structuring the community composition. 
      Instead, the parallel drifts and slight convergence of the two community 
      structures indicate that other environmental factors such as influent wastewater 
      composition and temperature may be more important. Care should be exercised in 
      interpreting the proportion of sequence reads as pyrosequencing may be biased as 
      compared to FISH. SIGNIFICANCE AND IMPACT OF THE STUDY: This study provides new 
      insights on the importance of indiscriminate high mortality rates brought by 
      external factors (here ozonation) on microbial community structures of activated 
      sludge system.
CI  - (c) 2014 The Society for Applied Microbiology.
FAU - Isazadeh, S
AU  - Isazadeh S
AD  - Department of Civil Engineering and Applied Mechanics, McGill University, 
      Montreal, QC, Canada.
FAU - Ozcer, P O
AU  - Ozcer PO
FAU - Frigon, D
AU  - Frigon D
LA  - eng
SI  - GENBANK/KC819805
SI  - GENBANK/KC819813
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140509
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Sewage)
RN  - 66H7ZZK23N (Ozone)
SB  - IM
MH  - Bacteria/*classification/genetics/isolation & purification
MH  - Molecular Sequence Data
MH  - *Ozone
MH  - Sewage/*microbiology
MH  - Temperature
MH  - Waste Disposal, Fluid
OTO - NOTNLM
OT  - activated sludge
OT  - community structure
OT  - fluorescence in situ hybridization
OT  - ozone
OT  - pyrosequencing
OT  - sludge reduction
EDAT- 2014/04/18 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/04/18 06:00
PHST- 2014/02/11 00:00 [received]
PHST- 2014/03/24 00:00 [revised]
PHST- 2014/04/07 00:00 [accepted]
PHST- 2014/04/18 06:00 [entrez]
PHST- 2014/04/18 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - 10.1111/jam.12523 [doi]
PST - ppublish
SO  - J Appl Microbiol. 2014 Aug;117(2):587-96. doi: 10.1111/jam.12523. Epub 2014 May 
      9.