PMID- 24739733
OWN - NLM
STAT- MEDLINE
DCOM- 20150528
LR  - 20211021
IS  - 1757-6512 (Electronic)
IS  - 1757-6512 (Linking)
VI  - 5
IP  - 2
DP  - 2014 Apr 16
TI  - Umbilical cord tissue-derived mesenchymal stem cells induce apoptosis in PC-3 
      prostate cancer cells through activation of JNK and downregulation of PI3K/AKT 
      signaling.
PG  - 54
LID - 10.1186/scrt443 [doi]
AB  - INTRODUCTION: Although mesenchymal stem cells (MSCs) have antitumor potential in 
      hepatocellular carcinoma and breast cancer cells, the antitumor mechanism of 
      human umbilical cord mesenchymal stem cells (hUCMSCs) in prostate cancer cells 
      still remains unclear. Thus, in the present study, we elucidated the antitumor 
      activity of hUCMSCs in PC-3 prostate cancer cells in vitro and in vivo. METHODS: 
      hUCMSCs were isolated from Wharton jelly of umbilical cord and characterized via 
      induction of differentiations, osteogenesis, and adipogenesis. Antitumor effects 
      of UCMSCs on tumor growth were evaluated in a co-culture condition with PC-3 
      prostate cancer cells. PC-3 cells were subcutaneously (sc) injected into the left 
      flank of nude mice, and UCMSCs were sc injected into the right flank of the same 
      mouse. RESULTS: We found that hUCMSCs inhibited the proliferation of PC-3 cells 
      in the co-culture condition. Furthermore, co-culture of hUCMSCs induced the 
      cleavage of caspase 9/3 and PARP, activated c-jun NH2-terminal kinase (JNK), and 
      Bax, and attenuated the phosphorylation of phosphatidylinositol 3-kinase (PI3K)/ 
      AKT, extracellular signal-regulated kinase (ERK), and the expression of survival 
      genes such as Bcl-2, Bcl-xL, Survivin, Mcl-1, and cIAP-1 in PC-3 cells in Western 
      blotting assay. Conversely, we found that treatment of specific JNK inhibitor 
      SP600125 suppressed the cleavages of caspase 9/3 and PARP induced by hUCMSCs in 
      PC-3 cells by Western blotting and immunofluorescence assay. The homing of 
      hUCMSCs to, and TUNEL-positive cells on, the K562 xenograft tumor region were 
      detected in Nu/nu-BALB/c mouse. CONCLUSIONS: These results suggest that UCMSCs 
      inhibit tumor growth and have the antitumor potential for PC-3 prostate cancer 
      treatment.
FAU - Han, Ihn
AU  - Han I
FAU - Yun, Miyong
AU  - Yun M
FAU - Kim, Eun-Ok
AU  - Kim EO
FAU - Kim, Bonglee
AU  - Kim B
FAU - Jung, Min-Hyung
AU  - Jung MH
FAU - Kim, Sung-Hoon
AU  - Kim SH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Retracted Publication
DEP - 20140416
PL  - England
TA  - Stem Cell Res Ther
JT  - Stem cell research & therapy
JID - 101527581
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 4)
SB  - IM
RIN - Stem Cell Res Ther. 2018 Dec 27;9(1):354. PMID: 30587247
MH  - Animals
MH  - Apoptosis/physiology
MH  - Cell Proliferation/physiology
MH  - Down-Regulation
MH  - Humans
MH  - MAP Kinase Kinase 4/*metabolism
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Phosphatidylinositol 3-Kinases/*metabolism
MH  - Prostatic Neoplasms/enzymology/pathology/*therapy
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - Signal Transduction
MH  - Umbilical Cord/cytology/*metabolism
PMC - PMC4055109
EDAT- 2014/04/18 06:00
MHDA- 2015/05/29 06:00
PMCR- 2014/04/16
CRDT- 2014/04/18 06:00
PHST- 2013/09/11 00:00 [received]
PHST- 2014/03/21 00:00 [accepted]
PHST- 2014/04/18 06:00 [entrez]
PHST- 2014/04/18 06:00 [pubmed]
PHST- 2015/05/29 06:00 [medline]
PHST- 2014/04/16 00:00 [pmc-release]
AID - scrt443 [pii]
AID - 10.1186/scrt443 [doi]
PST - epublish
SO  - Stem Cell Res Ther. 2014 Apr 16;5(2):54. doi: 10.1186/scrt443.