PMID- 24740533
OWN - NLM
STAT- MEDLINE
DCOM- 20141118
LR  - 20140721
IS  - 1545-5017 (Electronic)
IS  - 1545-5009 (Linking)
VI  - 61
IP  - 9
DP  - 2014 Sep
TI  - False positivity of ETV6/RUNX1 detected by FISH in healthy newborns and adults.
PG  - 1704-6
LID - 10.1002/pbc.25050 [doi]
AB  - The leukemia-associated ETV6-RUNX1-translocation frequently emerges prenatally. 
      Reverse-transcriptase PCR screening may indicate presence of ETV6-RUNX1 
      transcripts in random cord blood samples. Subsequent cell enrichment validation 
      finds significantly lower levels than validation applying fluorescence in situ 
      hybridization (FISH) (<10(-5) vs. 10(-3) to 10(-4)). Using three FISH probe sets, 
      we screened 179,000 cells from ETV6-RUNX1-positive dilution series, healthy 
      adults and random cord blood samples. The t(12;21) single fusion extra signal 
      translocation probe and the ETV6 break apart probe gave false positive results 
      mimicking ETV6-RUNX1-positive cell levels of 10(-3). This questions the paradigm 
      that 1% of newborns have ETV6-RUNX1-positive cells at levels of 10(-3) to 10(-4).
CI  - (c) 2014 Wiley Periodicals, Inc.
FAU - Kusk, Maria Schioldan
AU  - Kusk MS
AD  - Paediatrics and Adolescent Medicine, The University Hospital Rigshospitalet, 
      Copenhagen, Denmark.
FAU - Lausten-Thomsen, Ulrik
AU  - Lausten-Thomsen U
FAU - Andersen, Mette Klarskov
AU  - Andersen MK
FAU - Olsen, Marianne
AU  - Olsen M
FAU - Hjalgrim, Henrik
AU  - Hjalgrim H
FAU - Schmiegelow, Kjeld
AU  - Schmiegelow K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140417
PL  - United States
TA  - Pediatr Blood Cancer
JT  - Pediatric blood & cancer
JID - 101186624
RN  - 0 (Core Binding Factor Alpha 2 Subunit)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (TEL-AML1 fusion protein)
SB  - IM
MH  - Adult
MH  - Core Binding Factor Alpha 2 Subunit/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant, Newborn
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - Prognosis
MH  - Translocation, Genetic/*genetics
OTO - NOTNLM
OT  - acute lymphoblastic leukemia
OT  - etiology
OT  - fluorescence in situ hybridization
OT  - natural history
OT  - specificity
EDAT- 2014/04/18 06:00
MHDA- 2014/11/19 06:00
CRDT- 2014/04/18 06:00
PHST- 2013/04/11 00:00 [received]
PHST- 2014/03/07 00:00 [accepted]
PHST- 2014/04/18 06:00 [entrez]
PHST- 2014/04/18 06:00 [pubmed]
PHST- 2014/11/19 06:00 [medline]
AID - 10.1002/pbc.25050 [doi]
PST - ppublish
SO  - Pediatr Blood Cancer. 2014 Sep;61(9):1704-6. doi: 10.1002/pbc.25050. Epub 2014 
      Apr 17.