PMID- 24741571
OWN - NLM
STAT- MEDLINE
DCOM- 20141028
LR  - 20221207
IS  - 2314-6753 (Electronic)
IS  - 2314-6745 (Print)
VI  - 2014
DP  - 2014
TI  - Ethnic differences in microRNA-375 expression level and DNA methylation status in 
      type 2 diabetes of Han and Kazak populations.
PG  - 761938
LID - 10.1155/2014/761938 [doi]
LID - 761938
AB  - Han population is six times as likely as Kazak population to present with type 2 
      diabetes mellitus (T2DM) in China. We hypothesize that differential expression 
      and CpG methylation of miR-375 may be an ethnic-related factor that influences 
      the incidence of T2DM. The expression level of miR-375 was examined using 
      real-time PCR on Kazak and Han T2DM plasma samples. Furthermore, the methylation 
      levels of CpG sites of miR-375 promoter were determined by MassARRAY Spectrometry 
      in these samples. The relative expression levels of plasma miR-375 in Kazak T2DM 
      samples are 1, and the relative expression levels of plasma miR-375 in Han T2DM 
      samples are 3. The mean level of miR-375 methylation, calculated from the 
      methylation levels of the CpG sites, was 8.47% for the Kazak T2DM group and 
      10.38% for the Han T2DM group. Further, five CpG units showed a statistically 
      significant difference between Kazak and Han T2DM samples, and, among them, four 
      were hypomethylated and only one CpG unit showed hypermethylation in Kazak T2DM 
      samples. These findings indicate that the expression levels of plasma miR-375 and 
      its CpG methylation in the promoter region are ethnically different, which may 
      contribute to the different incidence of diabetes observed in Kazak and Han 
      populations.
FAU - Chang, Xiangyun
AU  - Chang X
AD  - Departments of Endocrinology and Metabolism, First Affiliated Hospital, Shihezi 
      University School of Medicine, Shihezi, Xinjiang 832002, China.
FAU - Li, Siyuan
AU  - Li S
AD  - Tongji Hospital, Tongji Medical College, Huazhong University of Science and 
      Technology, Wuhan 430030, China ; Shihezi University School of Medicine, Shihezi, 
      Xinjiang 832002, China.
FAU - Li, Jun
AU  - Li J
AD  - Departments of Endocrinology and Metabolism, First Affiliated Hospital, Shihezi 
      University School of Medicine, Shihezi, Xinjiang 832002, China.
FAU - Yin, Liang
AU  - Yin L
AD  - Departments of Endocrinology and Metabolism, First Affiliated Hospital, Shihezi 
      University School of Medicine, Shihezi, Xinjiang 832002, China.
FAU - Zhou, Ting
AU  - Zhou T
AD  - The Central Laboratory, First Affiliated Hospital, Shihezi University School of 
      Medicine, Shihezi, Xinjiang 832002, China.
FAU - Zhang, Chen
AU  - Zhang C
AD  - Departments of Endocrinology and Metabolism, First Affiliated Hospital, Shihezi 
      University School of Medicine, Shihezi, Xinjiang 832002, China.
FAU - Chen, Xuan
AU  - Chen X
AD  - Departments of Endocrinology and Metabolism, First Affiliated Hospital, Shihezi 
      University School of Medicine, Shihezi, Xinjiang 832002, China.
FAU - Sun, Kan
AU  - Sun K
AD  - Departments of Endocrinology and Metabolism, First Affiliated Hospital, Shihezi 
      University School of Medicine, Shihezi, Xinjiang 832002, China.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140311
PL  - England
TA  - J Diabetes Res
JT  - Journal of diabetes research
JID - 101605237
RN  - 0 (MIRN375 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Asian People
MH  - China/epidemiology
MH  - CpG Islands
MH  - *DNA Methylation
MH  - Diabetes Mellitus, Type 2/epidemiology/ethnology/*metabolism/physiopathology
MH  - Disease Progression
MH  - *Epigenesis, Genetic
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation
MH  - Hospitals, University
MH  - Humans
MH  - Incidence
MH  - MicroRNAs/blood/genetics/*metabolism
MH  - Prevalence
MH  - *Promoter Regions, Genetic
MH  - Prospective Studies
MH  - Reproducibility of Results
MH  - Risk Factors
PMC - PMC3972833
EDAT- 2014/04/18 06:00
MHDA- 2014/10/29 06:00
PMCR- 2014/03/11
CRDT- 2014/04/18 06:00
PHST- 2013/11/09 00:00 [received]
PHST- 2014/01/24 00:00 [revised]
PHST- 2014/02/08 00:00 [accepted]
PHST- 2014/04/18 06:00 [entrez]
PHST- 2014/04/18 06:00 [pubmed]
PHST- 2014/10/29 06:00 [medline]
PHST- 2014/03/11 00:00 [pmc-release]
AID - 10.1155/2014/761938 [doi]
PST - ppublish
SO  - J Diabetes Res. 2014;2014:761938. doi: 10.1155/2014/761938. Epub 2014 Mar 11.