PMID- 24742201
OWN - NLM
STAT- MEDLINE
DCOM- 20150202
LR  - 20160308
IS  - 1029-2454 (Electronic)
IS  - 0892-7014 (Linking)
VI  - 30
IP  - 6
DP  - 2014
TI  - Degradation of methicillin-resistant Staphylococcus aureus biofilms using a 
      chimeric lysin.
PG  - 667-74
LID - 10.1080/08927014.2014.905927 [doi]
AB  - Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for a large 
      number of chronic infections due to its ability to form robust biofilms. Herein, 
      the authors evaluated the anti-biofilm activity of a Staphylococcus specific 
      chimeric lysin ClyH on MRSA biofilms. ClyH is known to be active against 
      planktonic MRSA cells in vitro and in vivo. The minimum concentrations for 
      biofilm eradication (MCBE) of ClyH were 6.2-50 mg l(-1), much lower than those of 
      antibiotics. Scanning electron microscope (SEM) analysis revealed that ClyH 
      eliminated MRSA biofilms through cell lytic activity in a time-dependent manner. 
      Viable plate counts and kinetic analysis demonstrated that biofilms of different 
      ages displayed varying susceptibility to ClyH. Together with previously 
      demonstrated in vivo efficacy of ClyH against MRSA, the degradation efficacy 
      against biofilms of different ages indicates that ClyH could be used to remove 
      MRSA biofilms in vivo.
FAU - Yang, Hang
AU  - Yang H
AD  - a Center for Emerging Infectious Diseases and State Key Laboratory of Virology , 
      Wuhan Institute of Virology, Chinese Academy of Sciences , Wuhan , PR China.
FAU - Zhang, Yun
AU  - Zhang Y
FAU - Huang, Yanling
AU  - Huang Y
FAU - Yu, Junping
AU  - Yu J
FAU - Wei, Hongping
AU  - Wei H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140417
PL  - England
TA  - Biofouling
JT  - Biofouling
JID - 9200331
RN  - 0 (Mucoproteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (lysin, gastropoda)
RN  - J4Z741D6O5 (Gentian Violet)
SB  - IM
MH  - Analysis of Variance
MH  - Biofilms/*drug effects
MH  - Dose-Response Relationship, Drug
MH  - Gentian Violet
MH  - Methicillin-Resistant Staphylococcus aureus/*drug effects/ultrastructure
MH  - Microscopy, Electron, Scanning
MH  - Mucoproteins/*pharmacology
MH  - Recombinant Fusion Proteins/*pharmacology
OTO - NOTNLM
OT  - Staphylococcus aureus
OT  - anti-biofilm
OT  - biofilm
OT  - chimeric lysin
OT  - methicillin-resistant Staphylococcus aureus (MRSA)
OT  - susceptibility
EDAT- 2014/04/20 06:00
MHDA- 2015/02/03 06:00
CRDT- 2014/04/19 06:00
PHST- 2014/04/19 06:00 [entrez]
PHST- 2014/04/20 06:00 [pubmed]
PHST- 2015/02/03 06:00 [medline]
AID - 10.1080/08927014.2014.905927 [doi]
PST - ppublish
SO  - Biofouling. 2014;30(6):667-74. doi: 10.1080/08927014.2014.905927. Epub 2014 Apr 
      17.