PMID- 24742948
OWN - NLM
STAT- MEDLINE
DCOM- 20140710
LR  - 20211203
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 171
IP  - 1-2
DP  - 2014 Jun 25
TI  - Enterotoxigenic Escherichia coli infection induces intestinal epithelial cell 
      autophagy.
PG  - 160-4
LID - S0378-1135(14)00175-8 [pii]
LID - 10.1016/j.vetmic.2014.03.025 [doi]
AB  - The morbidity and mortality in piglets caused by enterotoxigenic Escherichia coli 
      (ETEC) results in large economic losses to the swine industry, but the precise 
      pathogenesis of ETEC-associated diseases remains unknown. Intestinal epithelial 
      cell autophagy serves as a host defense against pathogens. We found that ETEC 
      induced autophagy, as measured by both the increased punctae distribution of 
      GFP-LC3 and the enhanced conversion of LC3-I to LC3-II. Inhibiting autophagy 
      resulted in decreased survival of IPEC-1 cells infected with ETEC. ETEC triggered 
      autophagy in IPEC-1 cells through a pathway involving the mammalian target of 
      rapamycin (mTOR), the extracellular signal-regulated kinases 1/2 (ERK1/2), and 
      the AMP-activated protein kinase (AMPK).
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Tang, Yulong
AU  - Tang Y
AD  - Institute of Subtropical Agriculture, Chinese Academy of Sciences, Research 
      Center of Healthy Breeding Livestock & Poultry, Hunan Engineering & Research 
      Center of Animal & Poultry Science, Key Lab Agro-ecology Processing Subtropical 
      Region, Scientific Observational and Experimental Station of Animal Nutrition and 
      Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan, People's 
      Republic of China. Electronic address: tangyulong@isa.ac.cn.
FAU - Li, Fengna
AU  - Li F
AD  - Institute of Subtropical Agriculture, Chinese Academy of Sciences, Research 
      Center of Healthy Breeding Livestock & Poultry, Hunan Engineering & Research 
      Center of Animal & Poultry Science, Key Lab Agro-ecology Processing Subtropical 
      Region, Scientific Observational and Experimental Station of Animal Nutrition and 
      Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan, People's 
      Republic of China.
FAU - Tan, Bie
AU  - Tan B
AD  - Institute of Subtropical Agriculture, Chinese Academy of Sciences, Research 
      Center of Healthy Breeding Livestock & Poultry, Hunan Engineering & Research 
      Center of Animal & Poultry Science, Key Lab Agro-ecology Processing Subtropical 
      Region, Scientific Observational and Experimental Station of Animal Nutrition and 
      Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan, People's 
      Republic of China.
FAU - Liu, Gang
AU  - Liu G
AD  - Institute of Subtropical Agriculture, Chinese Academy of Sciences, Research 
      Center of Healthy Breeding Livestock & Poultry, Hunan Engineering & Research 
      Center of Animal & Poultry Science, Key Lab Agro-ecology Processing Subtropical 
      Region, Scientific Observational and Experimental Station of Animal Nutrition and 
      Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan, People's 
      Republic of China.
FAU - Kong, Xiangfeng
AU  - Kong X
AD  - Institute of Subtropical Agriculture, Chinese Academy of Sciences, Research 
      Center of Healthy Breeding Livestock & Poultry, Hunan Engineering & Research 
      Center of Animal & Poultry Science, Key Lab Agro-ecology Processing Subtropical 
      Region, Scientific Observational and Experimental Station of Animal Nutrition and 
      Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan, People's 
      Republic of China.
FAU - Hardwidge, Philip R
AU  - Hardwidge PR
AD  - Department of Diagnostic Medicine/Pathobiology, Kansas State University, 
      Manhattan, KS, USA.
FAU - Yin, Yulong
AU  - Yin Y
AD  - Institute of Subtropical Agriculture, Chinese Academy of Sciences, Research 
      Center of Healthy Breeding Livestock & Poultry, Hunan Engineering & Research 
      Center of Animal & Poultry Science, Key Lab Agro-ecology Processing Subtropical 
      Region, Scientific Observational and Experimental Station of Animal Nutrition and 
      Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan, People's 
      Republic of China. Electronic address: yinyulong@isa.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140330
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases/metabolism
MH  - Animals
MH  - Autophagy/*physiology
MH  - Cell Line
MH  - Enterotoxigenic Escherichia coli/*physiology
MH  - *Epithelial Cells/microbiology/pathology
MH  - Escherichia coli Infections/microbiology/pathology/*veterinary
MH  - *Intestines/microbiology/pathology
MH  - MAP Kinase Signaling System/physiology
MH  - Swine
MH  - Swine Diseases/microbiology/*pathology
MH  - TOR Serine-Threonine Kinases/metabolism
OTO - NOTNLM
OT  - Autophagy
OT  - Enterotoxigenic Escherichia coli
OT  - IPEC-1
EDAT- 2014/04/20 06:00
MHDA- 2014/07/11 06:00
CRDT- 2014/04/19 06:00
PHST- 2013/12/05 00:00 [received]
PHST- 2014/03/17 00:00 [revised]
PHST- 2014/03/22 00:00 [accepted]
PHST- 2014/04/19 06:00 [entrez]
PHST- 2014/04/20 06:00 [pubmed]
PHST- 2014/07/11 06:00 [medline]
AID - S0378-1135(14)00175-8 [pii]
AID - 10.1016/j.vetmic.2014.03.025 [doi]
PST - ppublish
SO  - Vet Microbiol. 2014 Jun 25;171(1-2):160-4. doi: 10.1016/j.vetmic.2014.03.025. 
      Epub 2014 Mar 30.