PMID- 24742951
OWN - NLM
STAT- MEDLINE
DCOM- 20140710
LR  - 20211021
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 171
IP  - 1-2
DP  - 2014 Jun 25
TI  - Effects of virulent and attenuated transmissible gastroenteritis virus on the 
      ability of porcine dendritic cells to sample and present antigen.
PG  - 74-86
LID - S0378-1135(14)00167-9 [pii]
LID - 10.1016/j.vetmic.2014.03.017 [doi]
AB  - Virulent transmissible gastroenteritis virus (TGEV) results in an acute, severe 
      pathology and high mortality in piglets, while attenuated TGEV only causes 
      moderate clinical reactions. Dendritic cells (DCs), through uptake and 
      presentation of antigens to T cells, initiate distinct immune responses to 
      different infections. In this study, an attenuated TGEV (STC3) and a virulent 
      TGEV (SHXB) were used to determine whether porcine DCs play an important role in 
      pathogenetic differences between these two TGEVs. Our results showed that 
      immature and mature monocyte-derived dendritic cells (Mo-DCs) were susceptible to 
      infection with SHXB and STC3. However, only SHXB inhibited Mo-DCs to activate 
      T-cell proliferation by down-regulating the expression of cell-surface markers 
      and the secretion of cytokines in vitro. In addition, after 48 h of SHXB 
      infection, there was the impairment in the ability of porcine intestinal DCs to 
      sample the antigen, to migrate from the villi to the lamina propria and to 
      activate T-cell proliferation in vivo. In contrast, these abilities of intestinal 
      DCs were enhanced in STC3-infected piglets. In conclusion, our results show that 
      SHXB significantly impaired the functions of Mo-DCs and intestinal DCs in vitro 
      and in vivo, while STC3 had the opposite effect. These differences may underlie 
      the pathogenesis of virulent and attenuated TGEV in piglets, and could help us to 
      develop a better strategy to prevent virulent TGEV infection.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Zhao, Shanshan
AU  - Zhao S
AD  - Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, College 
      of Veterinary Medicine,. Nanjing Agricultural University, Weigang 1, NanJing 
      210095, Jiangsu, PR China.
FAU - Gao, Qi
AU  - Gao Q
AD  - Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, College 
      of Veterinary Medicine,. Nanjing Agricultural University, Weigang 1, NanJing 
      210095, Jiangsu, PR China.
FAU - Qin, Tao
AU  - Qin T
AD  - Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, College 
      of Veterinary Medicine,. Nanjing Agricultural University, Weigang 1, NanJing 
      210095, Jiangsu, PR China.
FAU - Yin, Yinyan
AU  - Yin Y
AD  - Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, College 
      of Veterinary Medicine,. Nanjing Agricultural University, Weigang 1, NanJing 
      210095, Jiangsu, PR China.
FAU - Lin, Jian
AU  - Lin J
AD  - Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, College 
      of Veterinary Medicine,. Nanjing Agricultural University, Weigang 1, NanJing 
      210095, Jiangsu, PR China.
FAU - Yu, Qinghua
AU  - Yu Q
AD  - Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, College 
      of Veterinary Medicine,. Nanjing Agricultural University, Weigang 1, NanJing 
      210095, Jiangsu, PR China.
FAU - Yang, Qian
AU  - Yang Q
AD  - Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, College 
      of Veterinary Medicine,. Nanjing Agricultural University, Weigang 1, NanJing 
      210095, Jiangsu, PR China. Electronic address: zxbyq@njau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140327
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antigens, Viral)
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/*immunology
MH  - Cell Proliferation
MH  - Cytokines/metabolism
MH  - Dendritic Cells/cytology/immunology/*virology
MH  - Gastroenteritis, Transmissible, of Swine/immunology/pathology/*virology
MH  - Gene Expression Regulation/immunology
MH  - Intestines/pathology
MH  - Swine
MH  - T-Lymphocytes/cytology/immunology
MH  - *Transmissible gastroenteritis virus/immunology/pathogenicity
PMC - PMC7117177
OTO - NOTNLM
OT  - Intestinal DCs
OT  - Major histocompatibility complex classes II (SLA-II-DR)
OT  - Monocyte-derived dendritic cells (Mo-DCs)
OT  - T-cell proliferation
OT  - Virulent and attenuated transmissible gastroenteritis virus (TGEV)
EDAT- 2014/04/20 06:00
MHDA- 2014/07/11 06:00
PMCR- 2014/03/27
CRDT- 2014/04/19 06:00
PHST- 2013/12/18 00:00 [received]
PHST- 2014/03/10 00:00 [revised]
PHST- 2014/03/12 00:00 [accepted]
PHST- 2014/04/19 06:00 [entrez]
PHST- 2014/04/20 06:00 [pubmed]
PHST- 2014/07/11 06:00 [medline]
PHST- 2014/03/27 00:00 [pmc-release]
AID - S0378-1135(14)00167-9 [pii]
AID - 10.1016/j.vetmic.2014.03.017 [doi]
PST - ppublish
SO  - Vet Microbiol. 2014 Jun 25;171(1-2):74-86. doi: 10.1016/j.vetmic.2014.03.017. 
      Epub 2014 Mar 27.